Results 311 to 320 of about 1,454,497 (332)

Multiple‐primer DNA sequencing method

ELECTROPHORESIS, 2003
AbstractA multiple‐primer DNA sequencing approach suitable for genotyping, detection and identification of microorganisms and viruses has been developed. In this new method two or more sequencing primers, combined in a pool, are added to a DNA sample of interest. The oligonucleotide that hybridizes to the DNA sample will function as a primer during the
Pål Nyrén, Mehran Ghaderi, Baback Gharizadeh, Keng-Ling Wallin, Declan Donnelly, Bahram Amini   +5 more
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DNA sequencing by synthesis with degenerate primers

Journal of Genetics and Genomics, 2008
The degenerate primer-based sequencing was developed by a synthesis method (DP-SBS) for high-throughput DNA sequencing, in which a set of degenerate primers are hybridized on the arrayed DNA templates and extended by DNA polymerase on microarrays.
Xiaolong Shi, Chao Tang, Zuhong Lu, Xiujie Li   +3 more
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Fluorescent labeling of a DNA sequencing primer

DNA Sequence, 1993
Several oligonucleotides containing one to four fluorescein labels in various positions were synthesized and the fluorescence intensity and thermal stability of the duplex forms with their complementary sequences were measured. Oligomers that contain two fluorescein molecules, one attached at the 5' terminus and the other at an internal phosphate, were
Yoshizumi Ishino, Tadashi Ohminami, Ikunoshin Kato, Junichi Mineno   +3 more
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DNA Editing: A Primer

Blood, 2015
Abstract RNA-guided Cas9 nucleases based on the prokaryotic CRISPR-Cas systems provide an unprecedented ease in being able to edit the genomes of diverse organisms. As a single unifying factor capable of co-localizing RNA, DNA, and protein, I believe tools and techniques based on Cas9 will grant exquisite control over cellular ...
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Specific-primer-directed DNA sequencing

Analytical Biochemistry, 1986
A simple and rapid strategy for DNA sequence analysis based on the Sanger chain-termination method is described. This procedure utilizes full-sized inserts of 1 to 4 kb of DNA cloned into M13 bacteriophage vectors. After the sequence of the first 600-650 bp of the insert DNA has been determined with the commercially available universal vector primer, a
Joan A. Kobori, Gerald Siu, Erich Strauss, Leroy Hood   +3 more
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Nuclear DNA Primers

1998
Since its introduction in 1985, the polymerase chain reaction (PCR) (1) has profoundly affected the way in which molecular studies are conducted. Olsen et al. (2) first proposed that short single copy DNA sequences could be amplified by PCR from cloned DNA using specific primers.
Gordon C. Machray, Robbie Waugh, Joanne Russell   +2 more
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Are “universal” DNA primers really universal?

Journal of Applied Genetics, 2014
"Universal" DNA primers LCO 1490 and HCO 2198 were originally designed from three coding and six anticoding strands by comparing highly conserved regions of mitochondrial cytochrome c oxidase subunit I (COI) genes across 15 taxa. These primers have been successful in amplifying a 710-bp fragment of highly conserved regions of the COI gene for more than
Sharma, P., Kobayashi, T.
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An Environmental DNA Primer for Microbial and Restoration Ecology

Microbial Ecology, 2023
Environmental DNA (eDNA) sequencing-DNA collected from the environment from living cells or shed DNA-was first developed for working with microbes and has greatly benefitted microbial ecologists for decades since. These tools have only become increasingly powerful with the advent of metabarcoding and metagenomics.
Michael Tessler, Seth W. Cunningham, Melissa R. Ingala, Sally D. Warring, Mercer R. Brugler   +4 more
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Primers for Animal Mitochondrial DNA: The Importance of Species-Specific Primers

1998
In this chapter we outline the procedure for designing oligonucleotide primers for the direct-sequencing of in vitro amplified animal mitochondrial DNA (mtDNA). Although this chapter deals with animal mtDNA in particular, the approach is of course applicable to any gene, nuclear or mitochondrial. There are four main ‘steps’:identification of a suitable
Per Palsboll, P Arctander
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Introduction: DNA Basics—A Primer on DNA

2020
To better understand the contents of this book, the lay reader needs to know some basic facts about DNA, and the aim of the following brief introduction is to provide such a necessary background. The reader may also bump in the next chapters into some unusual terms so the Glossary at the end of this book should help him/her to understand the meaning of
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