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Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Science, 1988A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus , greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity ...
Randall Keichi Saiki +7 more
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DNA Synthesis by Primer Exchange Reaction Cascades [PDF]
ChemBioChem, 2018 Swap and extend: The autonomous synthesis of single-stranded DNA molecules of arbitrary size and sequence composition can easily be achieved by primer exchange reaction (PER) cascades, in which the sequential polymerase-mediated extension of DNA primers is guided by catalytic hairpins.
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Universal COI primers for DNA barcoding amphibians
Molecular Ecology Resources, 2011AbstractDNA barcoding is a proven tool for the rapid and unambiguous identification of species, which is essential for many activities including the vouchering tissue samples in the genome 10K initiative, genealogical reconstructions, forensics and biodiversity surveys, among many other applications.
Ya-Ping Zhang +10 more
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Effect of Irradiation of the Primer DNA on the Incorporation of Precursors
International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1961SummaryThe rate of enzymatic incorporation of 3H-labelled thymidine into primer DNA was studied after the DNA had been irradiated with doses up to 360 000 roentgens of gamma-rays. Non-irradiated DNA-synthesizing enzyme obtained from regenerating rat-liver was used together with DNA prepared by the method of Kay, Simmons and Dounce (1952) in the ...
C.M. Wheeler, S. Okada
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“Primer” in DNA Polymerase Reactions
1963Publisher Summary This chapter reviews two degrees of primer activation. The first concerns the secondary structure of the DNA molecule, and for the calf thymus and phage-induced E. coli polymerase this type of activation, typically produced by heat, acid, or alkaline denaturation, results in rate increases of one to two orders of magnitude.
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Ribosomal DNA Probes and Primers
1998Ribosomal DNA (rDNA) is the region of the genome coding for the RNA component of ribosomes. The ubiquity and conservation of rDNA sequences has made these DNA regions important tools for reconstructing phylogenetic relationships. In eukaryotes, rDNA sequences may be found in the nucleus and organelles.
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DNA Extraction and Primer Selection
2013Talk regarding pitfalls in DNA extraction and 16S amplicon primer choice when performing community analysis of complex microbial communities. The talk was a part of Workshop 2 "Principles, Potential, and Limitations of Novel Molecular Methods in Water Engineering; from Amplicon Sequencing to -omics Methods" given at the MEWE 2013 conference in Ann ...
Karst, Søren Michael +3 more
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DNA sequencing and primer extension mapping
1997The two DNA sequencing techniques currently in use are the chemical degradation method developed by Maxam and Gilbert (1979) and the enzymatic method developed by Sanger et al. (1977). Due to the less laborious protocol and the easy availability of oligonucleotides, the ‘Sanger’ method is now the most widely used one, being applicable not only for the ...
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Designing PCR Primer for DNA Methylation Mapping
2007DNA methylation is an epigenetic mechanism of gene regulation, and aberrant methylation has been associated with various types of diseases, especially cancers. Detection of DNA methylation has thus become an important approach for studying gene regulation and has potential diagnostic application.
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