Results 91 to 100 of about 3,869,166 (380)

Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

BMC Biotechnology, 2019
Background Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe
Syukri Shukor, Alfred Tamayo, Lorenzo Tosi, H. Benjamin Larman, Biju Parekkadan   +4 more
doaj   +1 more source

TRAF2 binds to TIFA via a novel motif and contributes to its autophagic degradation

FEBS Letters, EarlyView.
TRAF family members couple receptor signalling complexes to downstream outputs, but how they interact with these complexes is not always clear. Here, we show that during ADP‐heptose signalling, TRAF2 binding to TIFA requires two short sequence motifs in the C‐terminal tail of TIFA, which are distinct from the TRAF6 binding motif.
Tom Snelling, Kodi Hunter, Celest W. S. Tay, Nicola T. Wood, Philip Cohen   +4 more
wiley   +1 more source

A Careful Insight into DDI-Type Receptor Layers on the Way to Improvement of Click-Biology-Based Immunosensors

Biosensors
Protein-based microarrays are important tools for high-throughput medical diagnostics, offering versatile platforms for multiplex immunodetection. However, challenges arise in protein microarrays due to the heterogeneous nature of proteins and, thus ...
Sylwia Karoń, Marcin Drozd, Elżbieta Malinowska   +2 more
doaj   +1 more source

Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes

Scientific Reports, 2018
We have redesigned probes for in situ proximity ligation assay (PLA), resulting in more efficient localized detection of target proteins. In situ PLA depends on recognition of target proteins by pairs of antibody-oligonucleotide conjugates (PLA probes ...
Axel Klaesson, Karin Grannas, Tonge Ebai, Johan Heldin, Björn Koos, Mattias Leino, Doroteya Raykova, Johan Oelrich, Linda Arngården, Ola Söderberg, Ulf Landegren   +10 more
doaj   +1 more source

Shining a Spotlight on DNA: Single-Molecule Methods to Visualise DNA

Molecules, 2019
The ability to watch single molecules of DNA has revolutionised how we study biological transactions concerning nucleic acids. Many strategies have been developed to manipulate DNA molecules to investigate mechanical properties, dynamics and protein ...
Gurleen Kaur, Jacob S. Lewis, Antoine M. van Oijen   +2 more
doaj   +1 more source

Critical evaluation of the Illumina MethylationEPIC BeadChip microarray for whole-genome DNA methylation profiling

Genome Biology, 2016
In recent years the Illumina HumanMethylation450 (HM450) BeadChip has provided a user-friendly platform to profile DNA methylation in human samples. However, HM450 lacked coverage of distal regulatory elements.
R. Pidsley, E. Zotenko, Timothy J. Peters, M. Lawrence, G. Risbridger, P. Molloy, Susan Van Djik, B. Muhlhausler, C. Stirzaker, S. Clark   +9 more
semanticscholar   +1 more source

Sequence organization of feline leukemis virus DNA in infected cells [PDF]

, 1980
A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV.
Casey, James W., Davidson, Norman, Mullins, James I., Nicolson, Margery O.   +3 more
core   +1 more source

The epithelial barrier theory proposes a comprehensive explanation for the origins of allergic and other chronic noncommunicable diseases

FEBS Letters, EarlyView.
Exposure to common noxious agents (1), including allergens, pollutants, and micro‐nanoplastics, can cause epithelial barrier damage (2) in our body's protective linings. This may trigger an immune response to our microbiome (3). The epithelial barrier theory explains how this process can lead to chronic noncommunicable diseases (4) affecting organs ...
Can Zeyneloglu, Huseyn Babayev, Ismail Ogulur, Sena Ardicli, Yagiz Pat, Duygu Yazici, Bingjie Zhao, Lihong Chang, Xiaoqing Liu, Paolo D'Avino, Manru Li, Ceren Biçer, Fatma Hacer Kurtoğlu Babayev, Raja Dhir, Kari C. Nadeau, Marie‐Charlotte Brüggen, Mubeccel Akdis, Cezmi A. Akdis   +17 more
wiley   +1 more source

Profiling DNA methylation differences between inbred mouse strains on the Illumina Human Infinium MethylationEPIC microarray. [PDF]

PLoS ONE, 2018
The Illumina Infinium MethylationEPIC provides an efficient platform for profiling DNA methylation in humans at over 850,000 CpGs. Model organisms such as mice do not currently benefit from an equivalent array.
Hemant Gujar, Jane W Liang, Nicholas C Wong, Khyobeni Mozhui   +3 more
doaj   +1 more source

Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.

Proceedings of the National Academy of Sciences of the United States of America, 1996
A new and highly effective method, termed suppression subtractive hybridization (SSH), has been developed for the generation of subtracted cDNA libraries.
L. Diatchenko, Yun-Fai Chris Lau, Aaron P. Campbell, A. Chenchik, Fauzia Moqadam, B. C. Huang, S. Lukyanov, K. Lukyanov, N. Gurskaya, E. Sverdlov, P. Siebert   +10 more
semanticscholar   +1 more source