Results 111 to 120 of about 2,143,309 (303)

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Comparison between Taq DNA Polymerase and Its Stoffel Fragment for Quantitative Real-Time PCR with Hybridization Probes

BioTechniques, 2001
In quantitative real-time PCR assays, fluorophor-labeled oligonucleotide probes are employed to generate sequence-specific signals for the quantitative evaluation. Whereas TaqMan® probes have to be hydrolyzed during PCR by the endonucleolytic activity of
Jochen Wilhelm, Alfred Pingoud, Meinhard Hahn   +2 more
doaj   +1 more source

A fast algorithm for the construction of universal footprinting templates in DNA

, 2005
We introduce and give a complete description of a new graph to be used for DNA sequencing questions. This graph has the advantage over the classical de Bruijn graph that it fully accounts for the double stranded nature of DNA, rather than dealing with ...
James W. Anderson, Fox, Keith R., Graham A. Niblo, Anderson, James W., Keith R. Fox, Niblo, Graham A.   +5 more
core   +1 more source

The microbiome in human skin aging

FEBS Letters, EarlyView.
Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana, Cornelia Wiegand, Omid Omrani, Mario Fabri   +3 more
wiley   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

Engineered chromatin readers track damaged chromatin dynamics in live cells and animals

Nature Communications
DNA damage is a constant threat to genome integrity and function. Diminished capacity for DNA repair is linked to many human diseases, therefore, understanding the molecular pathways responding to DNA damage is key for developing novel therapies. Lack of
Richard Cardoso da Silva, Kristeli Eleftheriou, Davide C. Recchia, Vincent Portegijs, Douwe ten Bulte, Niklas Kupfer, Nathalie P. Vroegindeweij-de Wagenaar, Xabier Vergara, Ayoub Ouchene, Sander van den Heuvel, Tuncay Baubec   +10 more
doaj   +1 more source

all-probes

, 2011
FASTA-formatted text file of UCE-anchored probe sequences, designed from UCEs identified across Reptiles (birds and lizard). We (1) aligned these sequences to extant primate genomic sequences and (2) used synthetic oligos identical to a subset of these ...
Brant C. Faircloth (143432), McCormack, John E., Harvey, Michael G., Robb T. Brumfield (277631), Michael G. Harvey (277629), John E. McCormack (277628), Faircloth, Brant C., Crawford, Nicholas G., Nicholas G. Crawford (277630), Travis C. Glenn (121700), Glenn, Travis C., Brumfield, Robb T.   +11 more
core   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Development of a multiplex DNA-based traceability tool for crop plant materials [PDF]

, 2011
The authenticity of food is of increasing importance for producers, retailers and consumers. All groups benefit from the correct labelling of the contents of food products.
Theo W. Prins, Jeroen P. van Dijk, van Dijk, J.P., van Hoef, A.M.A., Kok, E.J., Ralf Seyfarth, Marleen M. Voorhuijzen, Seyfarth, R., A. M. Angeline Van Hoef, Hoef, A.M.A., van, Dijk, J.P., van, Voorhuijzen, M.M., Prins, T.W., Esther J. Kok   +13 more
core   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source