Results 1 to 10 of about 813,809 (116)

Digital Droplet Multiple Displacement Amplification (ddMDA) for Whole Genome Sequencing of Limited DNA Samples. [PDF]

open access: yesPLoS ONE, 2016
Multiple displacement amplification (MDA) is a widely used technique for amplification of DNA from samples containing limited amounts of DNA (e.g., uncultivable microbes or clinical samples) before whole genome sequencing.
Minsoung Rhee   +3 more
doaj   +3 more sources

Regions enriched for DNA repeats in chromosomes of Macrostomum mirumnovem, a species with a recent Whole Genome Duplication

open access: yesВавиловский журнал генетики и селекции, 2020
The free-living flatworm Macrostomum mirumnovem is a neopolyploid species whose genome underwent a recent Whole Genome Duplication (WGD). In the result of chromosome fusions of the ancient haploid chromosome set, large metacentric chromosomes were formed.
K. S. Zadesenets, N. B. Rubtsov
doaj   +1 more source

Molecular analysis through RAPD markers in greengram genotypes

open access: yesElectronic Journal of Plant Breeding, 2022
The objective of the study is to determine the genetic relationships and diversity among 20 genotypes of greengram. Ten randomly chosen decamers in total were screened, and all of them were amplified.
G. Kalaiyarasi* and S. Padmavathi
doaj   +1 more source

Validation of Half-Reaction Volumes of the Promega PowerPlex® Forensic Amplification Kits (PowerPlex® 18D Systems, PowerPlex ® 21System, PowerPlex® Fusion System and PowerPlex® Y23 System) in STR Analysis

open access: yesArab Journal of Forensic Sciences & Forensic Medicine, 2020
DNA amplification is known to be the most expensive step during forensic DNA analysis. This study evaluated the half-reaction amplification protocol (12.5 µL PCR product) using DNA amplification kits from Promega PowerPlex® (PowerPlex® 18D System ...
Hanan K. Mahmood   +8 more
doaj   +1 more source

Immunosuppressive activity of Campylobacter jejuni isolates in relation to the cellular link of the body’s immunoprotection

open access: yesUkrainian Journal of Veterinary Sciences, 2022
Global environmental changes have caused transformations in the biology of microorganisms, especially among campylobacter, which are currently associated with food toxic infections.
Tetiana Mazur   +2 more
doaj   +1 more source

Chimeric DNA byproducts in strand displacement amplification using the T7 replisome.

open access: yesPLoS ONE, 2022
Recent advances in next generation sequencing technologies enable reading DNA molecules hundreds of kilobases in length and motivate development of DNA amplification methods capable of producing long amplicons.
Dillon B Nye, Nathan A Tanner
doaj   +2 more sources

Fully Automated Lab-On-A-Disc Platform for Loop-Mediated Isothermal Amplification Using Micro-Carbon-Activated Cell Lysis

open access: yesSensors, 2020
Fast and fully automated deoxyribonucleic acid (DNA) amplification methods are of interest in the research on lab-on-a-disc (LOD) platforms because of their full compatibility with the spin-column mechanism using centrifugal force.
Moo-Jung Seo, Jae-Chern Yoo
doaj   +1 more source

Combined effects of temperature, pressure and low pH on the amplification of DNA of plant derived foods

open access: yesCzech Journal of Food Sciences, 2011
The effect of food processing on the DNA integrity was studied by means of PCR amplification of soybean, transgenic MON 810 and non-transgenic maize, bean, and pea.
Eva Bergerová   +2 more
doaj   +1 more source

Polymorphism of monogenic scab resistance loci in apple varieties

open access: yesТруды по прикладной ботанике, генетике и селекции, 2020
Background. Monogenic scab resistance is an important trait of apple, useful to plant breeders. DNA markers provide a possibility to differentiate apple cultivars according to individual resistance determinants with high reliability and identify ...
A. S. Lyzhin, N. N. Savel’eva
doaj   +1 more source

Improved PCR Amplification of Broad Spectrum GC DNA Templates. [PDF]

open access: yesPLoS ONE, 2016
Many applications in molecular biology can benefit from improved PCR amplification of DNA segments containing a wide range of GC content. Conventional PCR amplification of DNA sequences with regions of GC less than 30%, or higher than 70%, is complex due
Nicholas Guido   +3 more
doaj   +1 more source

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