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Isothermal Amplification Technology for Disease Diagnosis [PDF]
Biosensors, 2022 Isothermal amplification (IA) is a nucleic acid amplification technology (NAAT) that has contributed significantly to the healthcare system. The combination of NAAT with a suitable detection platform resulted in higher sensitivity, specificity, and rapid
Kiatnida Treerattrakoon +2 more
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A review of reaction enhancement strategies for isothermal nucleic acid amplification reactions
Sensors and Actuators Reports, 2021 Isothermal nucleic acid amplification techniques are used to detect a variety of molecules such as RNAs, DNAs and proteins. When compared to the gold standard polymerase chain reaction, isothermal methods are rapid and simple but often have lower ...
Stephanie Mccalla, Burcu Ozay
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Principles and Applications of Loop-Mediated Isothermal Amplification to Point-of-Care Tests
Biosensors, 2022 For the identification of nucleic acids, which are important biomarkers of pathogen-mediated diseases and viruses, the gold standard for NA-based diagnostic applications is polymerase chain reaction (PCR).
Jeewoong Park
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Advances in Isothermal Amplification for the Diagnosis of Tuberculosis. [PDF]
J Clin Lab AnalABSTRACT Background Detecting tuberculosis (TB) remains challenging in low‐resource settings due to limited access to advanced diagnostic tools. The emergence of isothermal amplification techniques, such as loop‐mediated isothermal amplification (LAMP), presents a promising solution for TB ...
Jirakittiwut N, Ratthawongjirakul P.
europepmc +3 more sources
Helicase‐dependent isothermal DNA amplification [PDF]
EMBO Reports, 2004 Polymerase chain reaction is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to separate two DNA strands. In vivo, DNA is replicated by DNA polymerases with various accessory proteins, including a DNA helicase that acts to separate duplex DNA.
Huimin Kong
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Diagnostic Devices for Isothermal Nucleic Acid Amplification [PDF]
Sensors, 2012 Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible.
Chia-Chen Chang +5 more
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A direct multiplex isothermal amplification-reverse dot blot hybridization system for β-thalassemia diagnosis [PDF]
Annals of HematologyMolecular diagnostic methods largely rely on expensive equipment and complex operations, which makes it difficult to achieve rapid and low-cost detection.
Chao Ye +8 more
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Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification
Molecules, 2015 The technique of loop-mediated isothermal amplification (LAMP) utilizes four (or six) primers targeting six (or eight) regions within a fairly small segment of a genome for amplification, with concentration higher than that used in traditional PCR ...
Peggy Tomasula, Jeffrey D Brewster
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Loop-Mediated Isothermal Amplification in Schistosomiasis [PDF]
Journal of Clinical Medicine, 2021 Human schistosomiasis is one of the most important parasitic diseases, causing around 250 million cases (mostly in Africa) and 280,000–500,000 deaths every year. Due to the limited resources and the far-removed nature of many endemic areas, the implementation of new, sensitive and specific diagnostic tools has had little success.
Juan García-Bernalt Diego +4 more
openaire +2 more sources
Sensors, 2021 The easy and rapid spread of bacterial contamination and the risk it poses to human health makes evident the need for analytical methods alternative to conventional time-consuming laboratory-based techniques for bacterial detection. To tackle this demand,
Sandra Leonardo +2 more
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