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Isothermal Amplification Technology for Disease Diagnosis [PDF]
Biosensors, 2022 Isothermal amplification (IA) is a nucleic acid amplification technology (NAAT) that has contributed significantly to the healthcare system. The combination of NAAT with a suitable detection platform resulted in higher sensitivity, specificity, and rapid
Poramin Boonbanjong +4 more
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A direct multiplex isothermal amplification-reverse dot blot hybridization system for β-thalassemia diagnosis [PDF]
Annals of HematologyMolecular diagnostic methods largely rely on expensive equipment and complex operations, which makes it difficult to achieve rapid and low-cost detection.
Chao Ye +8 more
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Diagnostic Devices for Isothermal Nucleic Acid Amplification [PDF]
Sensors, 2012 Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible.
Chia-Chen Chang +5 more
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Loop-Mediated Isothermal Amplification in Schistosomiasis [PDF]
Journal of Clinical Medicine, 2021 Human schistosomiasis is one of the most important parasitic diseases, causing around 250 million cases (mostly in Africa) and 280,000–500,000 deaths every year. Due to the limited resources and the far-removed nature of many endemic areas, the implementation of new, sensitive and specific diagnostic tools has had little success.
Juan García-Bernalt Diego +4 more
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Sensors, 2021 The easy and rapid spread of bacterial contamination and the risk it poses to human health makes evident the need for analytical methods alternative to conventional time-consuming laboratory-based techniques for bacterial detection. To tackle this demand,
Sandra Leonardo +2 more
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Foods, 2022 The isothermal amplification method, a molecular-based diagnostic technology, such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), is widely used as an alternative to the time-consuming and labor-intensive
Ye-Ji Moon, So-Young Lee, Se-Wook Oh
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Quantitative isothermal amplification on paper membranes using amplification nucleation site analysis [PDF]
Lab on a Chip, 2022 We report a novel method for the quantification of isothermal amplification reactions on paper membranes using discrete fluorescent amplification nucleation sites.
Benjamin P. Sullivan +7 more
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Molecular Mechanisms Underlying CRISPR/Cas-Based Assays for Nucleic Acid Detection
Current Issues in Molecular Biology, 2023 Applied to investigate specific sequences, nucleic acid detection assays can help identify novel bacterial and viral infections. Most up-to-date systems combine isothermal amplification with Cas-mediated detection.
Denis N. Antropov, Grigory A. Stepanov
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A review of reaction enhancement strategies for isothermal nucleic acid amplification reactions
Sensors and Actuators Reports, 2021 Isothermal nucleic acid amplification techniques are used to detect a variety of molecules such as RNAs, DNAs and proteins. When compared to the gold standard polymerase chain reaction, isothermal methods are rapid and simple but often have lower ...
Burcu Özay, Stephanie E McCalla
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Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests
Biosensors, 2023 Isothermal nucleic acid amplification tests have recently gained popularity over polymerase chain reaction (PCR), as they only require a constant temperature and significantly simplify nucleic acid amplification.
Jocelyn Reynolds +4 more
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