Results 1 to 10 of about 33,911 (295)

Solid phase isothermal amplification [PDF]

, 2019
The present invention relates to an amplification technology for multiple, rapid, sensitive, specific detection of nucleic acids in samples originating from the environment, water, food, animals, plants, microorganisms, cell cultures, or humans. In particular, the present invention relates to isothermal amplification and detection of nucleic acids ...
Wolff, Anders; id_orcid 0000-0001-9596-1215, Bang, Dang; id_orcid 0000-0001-8937-687X, Quyen, Than, Golabi, Mohsen; id_orcid 0000-0002-4332-1993, Dave, Vivek   +4 more
openaire   +2 more sources

Isothermal cross-priming amplification implementation study

Letters in Applied Microbiology, 2015
Abstract Cross-priming amplification (CPA) was evaluated for the early detection of norovirus (NV), rotavirus A (RV-A), enteric adenovirus (EAdV) and astrovirus (AstV). The analytical sensitivity of the CPA assay was 103 copies ml−1 for NV, RV-A and AstV detection and 104 copies ml−1 for EAdV detection.
Z, Bai, H, Xie, Q, You, S, Pickerill, Y, Zhang, T, Li, J, Geng, L, Hu, H, Shan, B, Di   +9 more
openaire   +2 more sources

ISOTHERMAL AMPLIFICATION FOR DETECTION OF PLUM POX VIRUS

Acta Horticulturae, 2008
A nucleic acid sequence-based amplification method coupled with flow-through hybridisation (NASBA-FH) was developed for Plum pox virus (PPV) detection. The detection limit of the NASBA-FH was established at two copies of control transcripts, resulting 10 times higher than that obtained by Co-PCR and 1000 times higher than the sensitivity afforded by RT-
Olmos, Antonio, Bertolini, Edson, Cambra, Mariano   +2 more
openaire   +2 more sources

Isothermal Amplification of Nucleic Acids

, 2021
Nucleic acid amplification technologies (NAATs) have become fundamental tools in molecular diagnostics, due to their ability to detect small amounts of target molecules. Since its development, Polymerase Chain Reaction (PCR) has been the most exploited method, being stablished as the “gold standard” technique for DNA amplification.
Oliveira, Beatriz B., Veigas, Bruno, Baptista, Pedro Viana   +2 more
openaire   +1 more source

Isothermal amplification using a chemical heating device for point-of-care detection of HIV-1.

PLoS ONE, 2012
BackgroundTo date, the use of traditional nucleic acid amplification tests (NAAT) for detection of HIV-1 DNA or RNA has been restricted to laboratory settings due to time, equipment, and technical expertise requirements.
Kelly A Curtis, Donna L Rudolph, Irene Nejad, Jered Singleton, Andy Beddoe, Bernhard Weigl, Paul LaBarre, S Michele Owen   +7 more
doaj   +1 more source

Strand displacement amplification—an isothermal,in vitroDNA amplification technique

Nucleic Acids Research, 1992
Strand Displacement Amplification (SDA) is an isothermal, in vitro nucleic acid amplification technique based upon the ability of HincII to nick the unmodified strand of a hemiphosphorothioate form of its recognition site, and the ability of exonuclease deficient klenow (exo- klenow) to extend the 3'-end at the nick and displace the downstream DNA ...
G T, Walker, M S, Fraiser, J L, Schram, M C, Little, J G, Nadeau, D P, Malinowski   +5 more
openaire   +3 more sources

One-step loop-mediated isothermal amplification system for Mycobacterium marinum detection

Microbiology Spectrum
Mycobacterium marinum is the etiological agent of cutaneous nontuberculous mycobacteriosis, inducing granuloma formation in human skin. Other mycobacterial species, including Mycobacterium ulcerans, Mycobacterium chelonae, and Mycobacterium fortuitum ...
Kayo Okumura, Yuji Miyamoto, Satoshi Mitarai, Manabu Ato   +3 more
doaj   +1 more source

Enhancing Biosecurity in Mollusc Aquaculture: A Review of Current Isothermal Nucleic Acid Detection Methods

Animals
The growing human population has increased the need for food beyond what terrestrial sources can provide. This boosts aquaculture demand for molluscs, fish, and crustaceans.
Hoda Abbas, Gemma Zerna, Alexandra Knox, Danielle Ackerly, Jacinta Agius, Karla Helbig, Travis Beddoe   +6 more
doaj   +1 more source

Establishment and application of isothermal multiple-self-matching-initiated amplification (IMSA) in detecting Type II heat-labile enterotoxin of Escherichia coli.

PLoS ONE, 2019
Enterotoxigenic Escherichia coli (ETEC) constitutes a major cause of diarrhea in young children and animals, particularly in poor regions of the world, as well the traveler's diarrhea in adult individuals.
Wenxin Liu, Chaowen Yuan, Liguo Zhang, Yufei Feng   +3 more
doaj   +1 more source

Establishment of Isothermal Rapid Detection Method for Salmon-derived Components in Food

Shipin gongye ke-ji
This article presented a visualization method for detecting salmon-derived components in Oncorhynchus mykiss and Salmo salar, based on fluorescence-labeled loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA).
Bo PENG, Lin HONG, Shiguang GAO, Wenchao CHEN, Xin ZHAO, Yanhong LIU, Qiuyue ZHENG   +6 more
doaj   +1 more source