Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis. [PDF]
Biochemistry (Mosc), 2020 Recently, there has been a rapid progress in the development of techniques for isothermal amplification of nucleic acids as an alternative to polymerase chain reaction (PCR). The advantage of these methods is that the nucleic acids amplification can be carried out at constant temperature, unlike PCR, which requires cyclic temperature changes. Moreover,
Bodulev OL, Sakharov IY.
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Mycoplasma pneumoniae: Current knowledge on nucleic acid amplification techniques and serological diagnostics [PDF]
Frontiers in Microbiology, 2016 Mycoplasma pneumoniae (M. pneumoniae) belongs to the class Mollicutes and has been recognized as a common cause of respiratory tract infections (RTIs), including community-acquired pneumonia (CAP), that occur worldwide and in all age groups. In addition,
Katherine eLoens, Margareta eIeven
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Application of nucleic acid signal amplification in biosensing and bioimaging
Biomedical TechnologyNucleic acid amplification techniques are broadly employed in nucleic acid testing due to their efficient accumulation of nucleic acid sequences. With the development of biotechnology, they are further applied to proteins, living cells, extracellular ...
Junqi Zhao +3 more
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Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection
Chemosensors, 2022 Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive.
Thi Ngoc Diep Trinh, Nae Yoon Lee
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Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR [PDF]
Practical Laboratory MedicineIsothermal nucleic acid amplification methods have gained prominence as a valuable innovation in molecular diagnostics. These methods function at a constant temperature without the requirement of thermal cycler apparatus, simplifying the amplification ...
Alazar Amare Amdiyee +1 more
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Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review
Biosensors, 2023 Nucleic acid amplification testing facilitates the detection of disease through specific genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early detection of microorganisms can alert early response systems to ...
Soo Min Lee +4 more
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The cost effectiveness of Nucleic Acid Amplification Techniques for the diagnosis of tuberculosis [PDF]
Respiratory Medicine, 2012 There is wide variation in the techniques deployed to diagnose tuberculosis in the UK, with little agreement on which tools or strategies are cost effective. This analysis therefore comprehensively evaluated the cost effectiveness of currently available diagnostic strategies for routine diagnosis of TB in the NHS.The analysis compared strategies ...
Hughes, Ralph +3 more
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Electrical and Electrochemical Monitoring of Nucleic Acid Amplification
Frontiers in Bioengineering and Biotechnology, 2015 Nucleic acid amplification is a gold standard technique for analyzing a tiny amount of nucleotides in molecular biology, clinical diagnostics, food safety, and environmental testing.
Tatsuro eGoda +2 more
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Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology
Micromachines, 2019 Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications.
Lena Gorgannezhad +2 more
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A review of reaction enhancement strategies for isothermal nucleic acid amplification reactions
Sensors and Actuators Reports, 2021 Isothermal nucleic acid amplification techniques are used to detect a variety of molecules such as RNAs, DNAs and proteins. When compared to the gold standard polymerase chain reaction, isothermal methods are rapid and simple but often have lower ...
Burcu Özay, Stephanie E McCalla
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