Mycoplasma pneumoniae: Current knowledge on nucleic acid amplification techniques and serological diagnostics [PDF]
Frontiers in Microbiology, 2016 Mycoplasma pneumoniae (M. pneumoniae) belongs to the class Mollicutes and has been recognized as a common cause of respiratory tract infections (RTIs), including community-acquired pneumonia (CAP), that occur worldwide and in all age groups. In addition,
Katherine eLoens, Margareta eIeven
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Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis. [PDF]
Biochemistry (Mosc), 2020 Recently, there has been a rapid progress in the development of techniques for isothermal amplification of nucleic acids as an alternative to polymerase chain reaction (PCR). The advantage of these methods is that the nucleic acids amplification can be carried out at constant temperature, unlike PCR, which requires cyclic temperature changes. Moreover,
Bodulev OL, Sakharov IY.
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Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR [PDF]
Practical Laboratory MedicineIsothermal nucleic acid amplification methods have gained prominence as a valuable innovation in molecular diagnostics. These methods function at a constant temperature without the requirement of thermal cycler apparatus, simplifying the amplification ...
Alazar Amare Amdiyee +1 more
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A review of reaction enhancement strategies for isothermal nucleic acid amplification reactions
Sensors and Actuators Reports, 2021 Isothermal nucleic acid amplification techniques are used to detect a variety of molecules such as RNAs, DNAs and proteins. When compared to the gold standard polymerase chain reaction, isothermal methods are rapid and simple but often have lower ...
Burcu Özay, Stephanie E McCalla
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Research progress on nucleic acid amplification–based detection technologies for phytopathogenic fungi [PDF]
Applied Microbiology and BiotechnologyPhytopathogenic fungi are highly diverse and globally distributed, posing a major threat to agricultural production worldwide. The annual losses caused by plant diseases can reach up to 30% of global crop yields, with over 80% of infections caused by ...
Baoyu Wang +5 more
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Design and Simulation Analysis of a Temperature Control System for Real-Time Quantitative PCR Instruments Based on Key Hot Air Circulation and Temperature Field Regulation Technologies [PDF]
MicromachinesTo address the technical bottlenecks commonly encountered with real-time quantitative PCR instruments, such as insufficient ramp rates and uneven chamber temperature distribution, this study proposes an innovative design scheme for a temperature control ...
Zhe Wang +9 more
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Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection
Chemosensors, 2022 Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive.
Thi Ngoc Diep Trinh, Nae Yoon Lee
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Molecular Mechanisms Underlying CRISPR/Cas-Based Assays for Nucleic Acid Detection
Current Issues in Molecular Biology, 2023 Applied to investigate specific sequences, nucleic acid detection assays can help identify novel bacterial and viral infections. Most up-to-date systems combine isothermal amplification with Cas-mediated detection.
Denis N. Antropov, Grigory A. Stepanov
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Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review
Biosensors, 2023 Nucleic acid amplification testing facilitates the detection of disease through specific genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early detection of microorganisms can alert early response systems to ...
Soo Min Lee +4 more
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Chemosensors, 2022 The determination of nucleic acids has become an analytical diagnostic method with many applications in fields such as biomedical sciences, environmental monitoring, forensic identification, and food safety.
Claudia Patiti +7 more
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