Results 41 to 50 of about 78,833 (285)

Prospective evaluation of BDProbeTec strand displacement amplification (SDA) system for diagnosis of tuberculosis in non-respiratory and respiratory samples

, 2004
Nucleic acid amplification techniques (NAATs) have been demonstrated to make significant improvements in the diagnosis of tuberculosis (TB), particularly in the time to diagnosis and the diagnosis of smear-negative TB.
Gillespie, S. H., Gosling, RD, Lipman, MC, McHugh, TD, Lipman, M. C., Ling, CL, Billington, O. J., Ling, C. L., Patel, S, Gosling, R. D., Gillespie, SH, McHugh, T. D., Patel, S., Pope, CF, Billington, OJ, Pope, C. F.   +15 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Cost analysis of nucleic acid amplification for diagnosing pulmonary tuberculosis, within the context of the Brazilian Unified Health Care System [PDF]

Jornal Brasileiro de Pneumologia, 2015
We estimated the costs of a molecular test for Mycobacterium tuberculosis and resistance to rifampin (Xpert MTB/RIF) and of smear microscopy, within the Brazilian Sistema Único de Saúde (SUS, Unified Health Care System). In SUS laboratories in the cities
Márcia Pinto, Aline Piovezan Entringer, Ricardo Steffen, Anete Trajman   +3 more
doaj   +3 more sources

Pipette-Tip-Enabled Digital Nucleic Acid Analyzer for COVID-19 Testing with Isothermal Amplification

, 2021
Herein, a pipette-tip-enabled digital nucleic acid analyzer for high-performance COVID-19 testing is demonstrated. This is achieved by digital loop-mediated isothermal amplification (digital LAMP or dLAMP) using common laboratory equipment and materials.
Xiaoyu Cheng (1810132), Jiali Ji (7905065), Wenyao Zhang, Kaixin Zheng, Yang Ye, Xiaoyu Cheng, Sailing He, Wenyao Zhang (149788), Kaixin Zheng (8906465), Yang Ye (175530), Jiali Ji, Sailing He (1423405)   +11 more
core   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Expert Consensus on Nucleic Acid of Amplification Techniques Test for the Diagnosis of Pathogens in Adult Respiratory Tract Infections(2023)

, 2023
Respiratory tract infection is one of the most prevalent medical conditions. Rapid and accurate diagnosis of pathogens is crucial for precise and effective treatment.
Chinese Thoracic Society of the Chinese Medical Association, The Committee of Chinese Laboratory Medical Education   +1 more
core   +1 more source

Blood Biomarkers and Surface‐Enhanced Raman Spectroscopy for Gout: A Comprehensive Review

Advanced Functional Materials, EarlyView.
Schematic illustrating gout disease progression from asymptomatic hyperuricemia to chronic tophaceous disease, highlighting the limitations of conventional imaging and biochemical diagnostics and the potential of engineered SERS platforms for ultrasensitive blood‐based detection of urate‐related biomarkers across disease stages, with the color gradient
Isuri Perera, Chiranjeevi Kanike, Appurva Tiwari, Hongyan Wu, Larry D. Unsworth, Ben Bin Xu, Xuehua Zhang   +6 more
wiley   +1 more source

Application of recombinase polymerase amplification with lateral flow assay to pathogen point-of-care diagnosis

Frontiers in Cellular and Infection Microbiology
Since the outbreak of the new coronavirus, point-of-care diagnostics based on nucleic acid testing have become a requirement for the development of pathogen diagnostics, which require the ability to accurately, rapidly, and conveniently detect pathogens.
Yilian Zhao, Yan Wei, Yan Wei, Chao Ye, Chao Ye, Jinmeng Cao, Xiaoxing Zhou, Xiaoxing Zhou, Mengru Xie, Jilin Qing, Zhizhong Chen   +10 more
doaj   +1 more source

Ultrasensitive Detection of Porcine Epidemic Diarrhea Virus Infections Using Multivalent DNA Nanostructure‐Enabled Lateral Flow Assay

Advanced Healthcare Materials, EarlyView.
A multivalent DNA nanostructure‐enabled lateral flow assay was developed for rapid, ultrasensitive detection of porcine epidemic diarrhea virus (PEDV) nucleocapsid protein. Designer net‐shaped DNA nanostructures (DNA‐Net) presenting PEDV‐specific aptamers achieved ~1000‐fold enhanced binding, enabling detection of viral copies with Ct ≤ 37.42 within 10
Saurabh Umrao, Akhila Naru, Dhanush Gandavadi, Mengxi Zheng, Saraswathi Lanka, Hyeongjun Cho, Chau Nguyen Minh Hoang, Jianqiang Zhang, Ying Fang, Xing Wang   +9 more
wiley   +1 more source

Research advances in the detection of miRNA

Journal of Pharmaceutical Analysis, 2019
MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as ...
Jiawei Ye, Mingcheng Xu, Xueke Tian, Sheng Cai, Su Zeng   +4 more
doaj   +1 more source