Simple system for isothermal DNA amplification coupled to lateral flow detection. [PDF]
PLoS ONE, 2013 Infectious disease diagnosis in point-of-care settings can be greatly improved through integrated, automated nucleic acid testing devices. We have developed an early prototype for a low-cost system which executes isothermal DNA amplification coupled to ...
Kristina Roskos +6 more
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Novel immuno-nucleic acid cooperative detection technology for food safety
Food and Agricultural Immunology, 2020 The establishment of nucleic acid amplification technology and functional nucleic acid aptamers promote the development of detection techniques. In particular, the detection displayed promise for broader application, as well as improved specificity ad ...
Xinyue Lan, Longjiao Zhu, Wentao Xu
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Advanced CRISPR-Cas Effector Enzyme-Based Diagnostics for Infectious Diseases, Including COVID-19
Life, 2021 Rapid and precise diagnostic tests can prevent the spread of diseases, including worldwide pandemics. Current commonly used diagnostic methods include nucleic-acid-amplification-based detection methods and immunoassays.
Sangha Kwon, Ha Youn Shin
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Heliyon, 2022 Zika virus (ZIKV) and Chikungunya virus (CHIKV) are arboviruses that cause important viral diseases affecting the world population. Both viruses can produce remarkably similar clinical manifestations, co-circulate in a geographic region, and coinfections
María C. Cardona-Trujillo +4 more
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Low-Cost 3D Printers Enable High-Quality and Automated Sample Preparation and Molecular Detection. [PDF]
PLoS ONE, 2016 Most molecular diagnostic assays require upfront sample preparation steps to isolate the target's nucleic acids, followed by its amplification and detection using various nucleic acid amplification techniques.
Kamfai Chan +8 more
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Recent Improvements in CRISPR-Based Amplification-Free Pathogen Detection
Frontiers in Microbiology, 2021 Molecular diagnostic (MDx) methods directly detect target nucleic acid sequences and are therefore an important approach for precise diagnosis of pathogen infection. In comparison with traditional MDx techniques such as PCR, the recently developed CRISPR-
Jian Zhang +9 more
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Clinical features of NAAT-positive, toxin-negative Clostridioides difficile infections in South Korea [PDF]
The Korean Journal of Internal MedicineBackground/Aims The clinical characteristics of Clostridioides difficile infection (CDI) in patients within the Asia-Pacific region who test positive on the nucleic acid amplification test (NAAT) and negative on the toxin enzyme immunoassay (EIA ...
In Hyoung Choi +3 more
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Recent advances and perspectives of nucleic acid detection for coronavirus
Journal of Pharmaceutical Analysis, 2020 The recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) is posing a great threat to global public health. Therefore, rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments, saving ...
Minzhe Shen +6 more
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Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches
Biosensors, 2023 Conventional diagnostic techniques are based on the utilization of analyte sampling, sensing and signaling on separate platforms for detection purposes, which must be integrated to a single step procedure in point of care (POC) testing devices.
Zilwa Mumtaz +6 more
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The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens
Foods, 2023 Today, with the globalization of the food trade progressing, food safety continues to warrant widespread attention. Foodborne diseases caused by contaminated food, including foodborne pathogens, seriously threaten public health and the economy.
Jinbin Zhao +7 more
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