Low-Cost 3D Printers Enable High-Quality and Automated Sample Preparation and Molecular Detection. [PDF]
PLoS ONE, 2016 Most molecular diagnostic assays require upfront sample preparation steps to isolate the target's nucleic acids, followed by its amplification and detection using various nucleic acid amplification techniques.
Kamfai Chan +8 more
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Clinical features of NAAT-positive, toxin-negative Clostridioides difficile infections in South Korea [PDF]
The Korean Journal of Internal MedicineBackground/Aims The clinical characteristics of Clostridioides difficile infection (CDI) in patients within the Asia-Pacific region who test positive on the nucleic acid amplification test (NAAT) and negative on the toxin enzyme immunoassay (EIA ...
In Hyoung Choi +3 more
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Recent Improvements in CRISPR-Based Amplification-Free Pathogen Detection
Frontiers in Microbiology, 2021 Molecular diagnostic (MDx) methods directly detect target nucleic acid sequences and are therefore an important approach for precise diagnosis of pathogen infection. In comparison with traditional MDx techniques such as PCR, the recently developed CRISPR-
Jian Zhang +9 more
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Recent advances and perspectives of nucleic acid detection for coronavirus
Journal of Pharmaceutical Analysis, 2020 The recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) is posing a great threat to global public health. Therefore, rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments, saving ...
Minzhe Shen +6 more
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Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches
Biosensors, 2023 Conventional diagnostic techniques are based on the utilization of analyte sampling, sensing and signaling on separate platforms for detection purposes, which must be integrated to a single step procedure in point of care (POC) testing devices.
Zilwa Mumtaz +6 more
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The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens
Foods, 2023 Today, with the globalization of the food trade progressing, food safety continues to warrant widespread attention. Foodborne diseases caused by contaminated food, including foodborne pathogens, seriously threaten public health and the economy.
Jinbin Zhao +7 more
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Nucleic acid purification from plants, animals and microbes in under 30 seconds.
PLoS Biology, 2017 Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples.
Yiping Zou +7 more
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Biosensors, 2023 Nucleic acid detection is widely used to identify infectious diseases and ensure food safety. However, conventional PCR-based techniques are time consuming.
Weidong Wu +3 more
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The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research
FEBS Letters, EarlyView.What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
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