Development of rapid, automated diagnostics for infectious disease: advances and challenges [PDF]
, 2009 The last 2 years has seen an exponential rise in the amount of research funding made available for the development of rapid diagnostic devices for infectious agents of medical importance. This review reports on several such projects.
Ince, J, McNally, A
core +1 more source
Electrical and Electrochemical Monitoring of Nucleic Acid Amplification
Frontiers in Bioengineering and Biotechnology, 2015 Nucleic acid amplification is a gold standard technique for analyzing a tiny amount of nucleotides in molecular biology, clinical diagnostics, food safety, and environmental testing.
Tatsuro eGoda +2 more
doaj +1 more source
Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics. [PDF]
, 2008 We demonstrate the first integrated microfluidic tmRNA purification and nucleic acid sequence-based amplification (NASBA) device incorporating real-time detection.
Antonio J. Ricco +48 more
core +1 more source
Improving statistical inference on pathogen densities estimated by quantitative molecular methods: malaria gametocytaemia as a case study [PDF]
, 2015 BACKGROUND: Quantitative molecular methods (QMMs) such as quantitative real-time polymerase chain reaction (q-PCR), reverse-transcriptase PCR (qRT-PCR) and quantitative nucleic acid sequence-based amplification (QT-NASBA) are increasingly used to ...
A Gelman +56 more
core +4 more sources
Recent trends in molecular diagnostics of yeast infections : from PCR to NGS [PDF]
, 2019 The incidence of opportunistic yeast infections in humans has been increasing over recent years. These infections are difficult to treat and diagnose, in part due to the large number and broad diversity of species that can underlie the infection.
Arastehfar, A +26 more
core +2 more sources
Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology
Micromachines, 2019 Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications.
Lena Gorgannezhad +2 more
doaj +1 more source
Low-Cost 3D Printers Enable High-Quality and Automated Sample Preparation and Molecular Detection. [PDF]
PLoS ONE, 2016 Most molecular diagnostic assays require upfront sample preparation steps to isolate the target's nucleic acids, followed by its amplification and detection using various nucleic acid amplification techniques.
Kamfai Chan +8 more
doaj +1 more source
Applications of Graphene Quantum Dots in Biomedical Sensors [PDF]
, 2020 Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow ...
Altintas, Zeynep, Mansuriya, Bhargav D.
core +1 more source
Recent Improvements in CRISPR-Based Amplification-Free Pathogen Detection
Frontiers in Microbiology, 2021 Molecular diagnostic (MDx) methods directly detect target nucleic acid sequences and are therefore an important approach for precise diagnosis of pathogen infection. In comparison with traditional MDx techniques such as PCR, the recently developed CRISPR-
Jian Zhang +9 more
doaj +1 more source
Recent advances and perspectives of nucleic acid detection for coronavirus
Journal of Pharmaceutical Analysis, 2020 The recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) is posing a great threat to global public health. Therefore, rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments, saving ...
Minzhe Shen +6 more
doaj +1 more source