Nucleic acid amplification techniques for the detection of Schistosoma mansoni infection in humans and the intermediate snail host: a structured review and meta-analysis of diagnostic accuracy [PDF]
International Journal of Infectious Diseases, 2021 Background: Schistosomiasis is a parasitic disease caused by hematodes of genus Schistosoma. This review evaluated the available nucleic acid amplification techniques for diagnosing S.
Hong-Mei Li +8 more
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Research progress on nucleic acid amplification–based detection technologies for phytopathogenic fungi [PDF]
Applied Microbiology and BiotechnologyPhytopathogenic fungi are highly diverse and globally distributed, posing a major threat to agricultural production worldwide. The annual losses caused by plant diseases can reach up to 30% of global crop yields, with over 80% of infections caused by ...
Baoyu Wang +5 more
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Nucleic acid amplification-based techniques for pathogen detection and identification
Infection, Genetics and Evolution, 2006 Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and ...
Paul Monis
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Chip-Based and Wearable Tools for Isothermal Amplification and Electrochemical Analysis of Nucleic Acids [PDF]
Chemosensors, 2022 The determination of nucleic acids has become an analytical diagnostic method with many applications in fields such as biomedical sciences, environmental monitoring, forensic identification, and food safety.
Claudia Patiti +7 more
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Biosensors and Bioelectronics: X, 2023 The recent emergence of human coronaviruses (CoVs) causing severe acute respiratory syndrome (SARS) is posing a great threat to global public health. Therefore, the rapid and accurate identification of pathogenic viruses plays a vital role in selecting ...
Xucheng Huang +4 more
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Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms
BiosensorsRibonucleic acid (RNA) viruses are one of the major classes of pathogens that cause human diseases. The conventional method to detect RNA viruses is real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), but it has some ...
Le Thi Nhu Ngoc, Young-Chul Lee
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Nucleic Acid-Based Sensing Techniques for Diagnostics and Surveillance of Influenza
Biosensors, 2021 Influenza virus poses a threat to global health by causing seasonal outbreaks as well as three pandemics in the 20th century. In humans, disease is primarily caused by influenza A and B viruses, while influenza C virus causes mild disease mostly in ...
Samantha J. Courtney +2 more
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Molecular Mechanisms Underlying CRISPR/Cas-Based Assays for Nucleic Acid Detection
Current Issues in Molecular Biology, 2023 Applied to investigate specific sequences, nucleic acid detection assays can help identify novel bacterial and viral infections. Most up-to-date systems combine isothermal amplification with Cas-mediated detection.
Denis N. Antropov, Grigory A. Stepanov
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A comparison of nucleic acid amplification techniques for the assessment of bacterial viability [PDF]
Letters in Applied Microbiology, 2001 The ability to determine the presence and viability status of bacteria by molecular methods could offer significant advantages to the food, environmental and health sectors, in terms of improved speed and sensitivity of detection.In this study, we have assessed three amplification techniques, PCR, RT-PCR and NASBA, for their ability to detect nucleic ...
L, Birch +3 more
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A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain
Synthetic and Systems Biotechnology, 2023 Nucleic acid detection plays a key role in diverse diagnosis and disease control. Currently available nucleic acid detection techniques are challenged by trade-offs among speed, simplicity, precision and cost.
Yuting Shuai +10 more
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