Results 31 to 40 of about 78,833 (285)
Exploration of isothermal nucleic acid amplification techniques in the biomedical field
Gene and Genome EditingRajat Varshney, Sourabh Sulabh
exaly +2 more sources
Nucleic acid purification from plants, animals and microbes in under 30 seconds.
PLoS Biology, 2017 Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples.
Yiping Zou +7 more
doaj +1 more source
Biosensors, 2023 Nucleic acid detection is widely used to identify infectious diseases and ensure food safety. However, conventional PCR-based techniques are time consuming.
Weidong Wu +3 more
doaj +1 more source
Detection of Nucleic Acids with a Novel Stem-Loop Primer Rolling Circle Amplification Technique [PDF]
BioTechniques, 2018 This paper presents a new rolling circle amplification (RCA) technique using stem-loop primers (SLP). The technique enables detection of target DNA by either linear or exponential amplification (SLP-lRCA and SLP-eRCA) in both liquid and solid phases. For solid-phase detection, SLP-eRCA detects nucleic acids in four steps: (1) covalently immobilize an ...
Beibei Zhang +4 more
openaire +3 more sources
Unexpected detection of DNA by nucleic acid sequence-based amplification technique
Molecular and Cellular Probes, 2004 Nucleic acid sequence-based amplification (NASBA) is a technique that has been previously shown to selectively mediate the detection of RNA in microbial cells. In a series of tests, nucleic acids were extracted from Salmonella enterica serotype Typhimurium and Mycobacterium avium subsp. paratuberculosis, and subjected to four enzymatic treatments prior
David, Rodríguez-Làzaro +4 more
openaire +2 more sources
Molecular Oncology, EarlyView.A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel +12 more
wiley +1 more source
A Rapid and Sensitive Nucleic Acid Amplification Technique for Mycoplasma Screening of Cell Therapy Products [PDF]
Molecular Therapy - Methods & Clinical Development, 2020 Mycoplasma species (spp.) bacteria can infect cell cultures, posing a potential threat to recipients of cell therapy products. Conventional Mycoplasma testing methods are highly sensitive but typically require a minimum of 28 days to produce results. This delay is problematic if rapid results are needed to inform treatment decisions.
Lisa Dreolini +9 more
openaire +3 more sources
Development and clinical performance of nucleic acid amplification techniques for the diagnosis of Strongyloides stercoralis [PDF]
, 2017 The laboratory diagnosis of Strongyloides stercoralis (S. stercoralis) at the Department of Clinical Parasitology (DCP) by the routine methods of microscopy and Strongyloides culture is not sensitive due to the, usually, low parasite load and ...
Bowers, K.
core
The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?
, 2007 Museums and pathology collections around the world represent an archive of genetic material to study populations and diseases. For preservation purposes, a large portion of these collections has been fixed in formalin-containing solutions, a treatment ...
Haselkorn, Tamara +35 more
core +1 more source
Molecular Oncology, EarlyView.The proposed mechanism of action for the CDK12/13 inhibitor and cyclin K degrader, CT7439. CDK12/13 inhibition interrupts transcription elongation, leading to increased DNA damage that results in cell death. This agent is a potentially novel treatment option for patients with colorectal cancer. Created in BioRender. Cyclin‐dependent kinase (CDK) 12 and
Wylie K. Watlington +10 more
wiley +1 more source