Results 31 to 40 of about 187,051 (303)

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

Biosensors, 2023
Conventional diagnostic techniques are based on the utilization of analyte sampling, sensing and signaling on separate platforms for detection purposes, which must be integrated to a single step procedure in point of care (POC) testing devices.
Zilwa Mumtaz, Zubia Rashid, Ashaq Ali, Afsheen Arif, Fuad Ameen, Mona S. AlTami, Muhammad Zubair Yousaf   +6 more
doaj   +1 more source

Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeriamonocytogenes in food [PDF]

, 2009
We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with
Amerongen, A., van, Blazkova, M., Koets, M., Rauch, P.   +3 more
core   +2 more sources

Clinical features of NAAT-positive, toxin-negative Clostridioides difficile infections in South Korea [PDF]

The Korean Journal of Internal Medicine
Background/Aims The clinical characteristics of Clostridioides difficile infection (CDI) in patients within the Asia-Pacific region who test positive on the nucleic acid amplification test (NAAT) and negative on the toxin enzyme immunoassay (EIA ...
In Hyoung Choi, Chang Kyo Oh, Young Wook Cho, Young-Seok Cho   +3 more
doaj   +1 more source

tmRNA - a novel high-copy-number RNA diagnostic target - its application for Staphylococcus aureus detection using real-time NASBA [PDF]

, 2009
A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 ...
Anonymous, Anonymous, Anonymous, Anonymous, Barry Glynn, Justin O'Grady, Katrina Lacey, Majella Maher, Terry J. Smith, Thomas Barry   +9 more
core   +1 more source

The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens

Foods, 2023
Today, with the globalization of the food trade progressing, food safety continues to warrant widespread attention. Foodborne diseases caused by contaminated food, including foodborne pathogens, seriously threaten public health and the economy.
Jinbin Zhao, Yulan Guo, Xueer Ma, Shitong Liu, Chunmeng Sun, Ming Cai, Yuyang Chi, Kun Xu   +7 more
doaj   +1 more source

Real-time PCR quantitation of hepatitis B virus DNA using automated sample preparation and murine cytomegalovirus internal control [PDF]

, 2005
Quantitation of circulating hepatitis B virus (HBV) DNA is important for monitoring disease progression and for assessing the response to antiviral therapy. Several commercial and 'in house' assays for HBV DNA quantitation have been described but many of
Ayliffe, U, Ferns, RB, Garson, JA, Grant, PR, Tedder, RS   +4 more
core   +1 more source

Unexpected detection of DNA by nucleic acid sequence-based amplification technique

Molecular and Cellular Probes, 2004
Nucleic acid sequence-based amplification (NASBA) is a technique that has been previously shown to selectively mediate the detection of RNA in microbial cells. In a series of tests, nucleic acids were extracted from Salmonella enterica serotype Typhimurium and Mycobacterium avium subsp. paratuberculosis, and subjected to four enzymatic treatments prior
David, Rodríguez-Làzaro, Joy, Lloyd, John, Ikonomopoulos, Maria, Pla, Nigel, Cook   +4 more
openaire   +2 more sources

Nucleic acid purification from plants, animals and microbes in under 30 seconds.

PLoS Biology, 2017
Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples.
Yiping Zou, Michael Glenn Mason, Yuling Wang, Eugene Wee, Conny Turni, Patrick J Blackall, Matt Trau, Jose Ramon Botella   +7 more
doaj   +1 more source

Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection [PDF]

, 2009
We report the design, fabrication, and characterization of a polymer centrifugal microfluidic system for the specific detection of bacterial pathogens.
Barry, Thomas, Dimov, Ivan Krastev, Ducrée, Jens, Garcia-Cordero, Jose L., O'Grady, J., Ricco, Antonio J.   +5 more
core   +1 more source

Advances in Microfluidics and Lab-on-a-Chip Technologies

, 2017
Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense.
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core   +1 more source