Results 1 to 10 of about 210,277 (116)

Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR [PDF]

Practical Laboratory Medicine
Isothermal nucleic acid amplification methods have gained prominence as a valuable innovation in molecular diagnostics. These methods function at a constant temperature without the requirement of thermal cycler apparatus, simplifying the amplification ...
Alazar Amare Amdiyee, Tesfaye Sisay Tessema   +1 more
doaj   +2 more sources

Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection

Chemosensors, 2022
Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive.
Thi Ngoc Diep Trinh, Nae Yoon Lee
doaj   +3 more sources

Application of nucleic acid signal amplification in biosensing and bioimaging

Biomedical Technology
Nucleic acid amplification techniques are broadly employed in nucleic acid testing due to their efficient accumulation of nucleic acid sequences. With the development of biotechnology, they are further applied to proteins, living cells, extracellular ...
Junqi Zhao, Xueqin Li, Dagan Zhang, Sen Wang   +3 more
doaj   +3 more sources

Research progress on nucleic acid amplification–based detection technologies for phytopathogenic fungi [PDF]

Applied Microbiology and Biotechnology
Phytopathogenic fungi are highly diverse and globally distributed, posing a major threat to agricultural production worldwide. The annual losses caused by plant diseases can reach up to 30% of global crop yields, with over 80% of infections caused by ...
Baoyu Wang, Mei Liu, Hui Wang, Chenfang Wang, Wei Zhang, Jiye Yan   +5 more
doaj   +2 more sources

Locked nucleic acid pentamers as universal PCR primers for genomic DNA amplification. [PDF]

PLoS ONE, 2008
BACKGROUND: Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods.
Zhen Sun, Zhi Chen, Xiaoli Hou, Shuping Li, Haihong Zhu, Ji Qian, Daru Lu, Wei Liu   +7 more
doaj   +1 more source

Isothermal amplification using sequence-specific fluorescence detection of SARS coronavirus 2 and variants in nasal swabs

BioTechniques, 2022
Coronavirus disease 2019 is a public health challenge requiring rapid testing for the detection of infections and transmission. Nucleic acid amplification tests targeting SARS coronavirus 2 (CoV2) are used to detect CoV2 in clinical samples.
Les Jones, Hemant K Naikare, Yung-Yi C Mosley, Ralph A Tripp   +3 more
doaj   +1 more source

Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications

Frontiers in Cellular and Infection Microbiology, 2022
After the outbreak of SARS-CoV-2, nucleic acid testing quickly entered people’s lives. In addition to the polymerase chain reaction (PCR) which was commonly used in nucleic acid testing, isothermal amplification methods were also important nucleic acid ...
Meiying Tan, Chuan Liao, Lina Liang, Xueli Yi, Zihan Zhou, Guijiang Wei   +5 more
doaj   +1 more source

Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP

Micromachines, 2021
This paper presents a universal point-of-care system for fully automated quantification of human T-cell lymphotropic virus type 1 (HTLV-1) proviral load, including genomic RNA, based on digital reverse RNA transcription and c-DNA amplification by MD LAMP
Lisa Becherer, Jacob Friedrich Hess, Sieghard Frischmann, Mohammed Bakheit, Hans Nitschko, Silvina Stinco, Friedrich Zitz, Hannes Hofer, Giampiero Porro, Florian Hausladen, Karl Stock, Dominik Drossart, Holger Wurm, Hanna Kuhn, Dominik Huber, Tobias Hutzenlaub, Nils Paust, Mark Keller, Oliver Strohmeier, Simon Wadle, Nadine Borst, Roland Zengerle, Felix von Stetten   +22 more
doaj   +1 more source

Novel bioluminescent quantitative detection of nucleic acid amplification in real-time. [PDF]

PLoS ONE, 2010
BACKGROUND: The real-time monitoring of polynucleotide amplification is at the core of most molecular assays. This conventionally relies on fluorescent detection of the amplicon produced, requiring complex and costly hardware, often restricting it to ...
Olga A Gandelman, Vicki L Church, Cathy A Moore, Guy Kiddle, Christopher A Carne, Surendra Parmar, Hamid Jalal, Laurence C Tisi, James A H Murray   +8 more
doaj   +1 more source

All-in-One Digital Microfluidics System for Molecular Diagnosis with Loop-Mediated Isothermal Amplification

Biosensors, 2022
In this study, an “all-in-one” digital microfluidics (DMF) system was developed for automatic and rapid molecular diagnosis and integrated with magnetic bead-based nucleic acid extraction, loop-mediated isothermal amplification (LAMP), and real-time ...
Siyi Hu, Yuhan Jie, Kai Jin, Yifan Zhang, Tianjie Guo, Qi Huang, Qian Mei, Fuqiang Ma, Hanbin Ma   +8 more
doaj   +1 more source