Results 11 to 20 of about 210,426 (263)

Electrical and Electrochemical Monitoring of Nucleic Acid Amplification

open access: yesFrontiers in Bioengineering and Biotechnology, 2015
Nucleic acid amplification is a gold standard technique for analyzing a tiny amount of nucleotides in molecular biology, clinical diagnostics, food safety, and environmental testing.
Tatsuro eGoda   +2 more
doaj   +1 more source

Novel immuno-nucleic acid cooperative detection technology for food safety

open access: yesFood and Agricultural Immunology, 2020
The establishment of nucleic acid amplification technology and functional nucleic acid aptamers promote the development of detection techniques. In particular, the detection displayed promise for broader application, as well as improved specificity ad ...
Xinyue Lan, Longjiao Zhu, Wentao Xu
doaj   +1 more source

Molecular Mechanisms Underlying CRISPR/Cas-Based Assays for Nucleic Acid Detection

open access: yesCurrent Issues in Molecular Biology, 2023
Applied to investigate specific sequences, nucleic acid detection assays can help identify novel bacterial and viral infections. Most up-to-date systems combine isothermal amplification with Cas-mediated detection.
Denis N. Antropov, Grigory A. Stepanov
doaj   +1 more source

Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices

open access: yesBiosensors, 2012
Diagnostic tools for biomolecular detection need to fulfill specific requirements in terms of sensitivity, selectivity and high-throughput in order to widen their applicability and to minimize the cost of the assay.
Giuseppe Spoto, Laura Maria Zanoli
doaj   +1 more source

Nucleic acid amplification: Alternative methods of polymerase chain reaction

open access: yesJournal of Pharmacy and Bioallied Sciences, 2013
Nucleic acid amplification is a valuable molecular tool not only in basic research but also in application oriented fields, such as clinical medicine development, infectious diseases diagnosis, gene cloning and industrial quality control.
Md Fakruddin   +6 more
doaj   +1 more source

Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection

open access: yesBiosensors, 2023
Pathogenic pathogens invade the human body through various pathways, causing damage to host cells, tissues, and their functions, ultimately leading to the development of diseases and posing a threat to human health.
Yue Wang   +7 more
doaj   +1 more source

By-product formation in repetitive PCR amplification of DNA libraries during SELEX. [PDF]

open access: yesPLoS ONE, 2014
The selection of nucleic acid aptamers is an increasingly important approach to generate specific ligands binding to virtually any molecule of choice. However, selection-inherent amplification procedures are prone to artificial by-product formation that ...
Fabian Tolle   +3 more
doaj   +1 more source

Cartridge Based Nucleic Acid Amplification Test: The Sherlock of Tuberculosis

open access: yesJournal of Pure and Applied Microbiology, 2019
Emergence of Tuberculosis and Multi drug resistance tuberculosis at higher pace in the population needs rapid diagnosis with accuracy to reduce the transmission of disease and suffering of the patients. The technique of GenexpertMTb/Rif by Cepheid rolled
Jureka Mankotia   +2 more
doaj   +1 more source

Digital Microfluidics for Nucleic Acid Amplification

open access: yesSensors, 2017
Digital Microfluidics (DMF) has emerged as a disruptive methodology for the control and manipulation of low volume droplets. In DMF, each droplet acts as a single reactor, which allows for extensive multiparallelization of biological and chemical ...
Beatriz Coelho   +6 more
doaj   +1 more source

Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review

open access: yesBiosensors, 2023
Nucleic acid amplification testing facilitates the detection of disease through specific genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early detection of microorganisms can alert early response systems to ...
Soo Min Lee   +4 more
doaj   +1 more source

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