Results 1 to 10 of about 36,229 (210)

Rapid detection of Mycobacterium tuberculosis by recombinase polymerase amplification. [PDF]

PLoS ONE, 2014
Improved access to effective tests for diagnosing tuberculosis (TB) has been designated a public health priority by the World Health Organisation. In high burden TB countries nucleic acid based TB tests have been restricted to centralised laboratories ...
David S Boyle, Ruth McNerney, Hwee Teng Low, Brandon Troy Leader, Ailyn C Pérez-Osorio, Jessica C Meyer, Denise M O'Sullivan, David G Brooks, Olaf Piepenburg, Matthew S Forrest   +9 more
doaj   +11 more sources

Recombinase polymerase amplification of forensic short tandem repeat loci [PDF]

Scientific Reports
Short tandem repeats (STRs) are highly polymorphic repetitive DNA sequences extensively used in forensic science for identification of individuals. STR genotyping is usually performed by capillary electrophoresis (CE) or next-generation sequencing (NGS ...
Sonja Škevin, Liesl De Keyzer, Lynn De Waele, Olivier Tytgat, David Van Hoofstat, Dieter Deforce, Filip Van Nieuwerburgh   +6 more
doaj   +3 more sources

Establishment of recombinase polymerase amplification detection method for Dactylobotrys graminicola [PDF]

Scientific Reports
Hulless barley sheath rot is a spike disease caused by Dactylobotrys graminicola. In recent years, it has generally occurred in hulless barley growing areas in China, resulting in reduced hulless barley yields.
Haiqing Zhang, Liyifan Chen, Ruifang Dong, Haowen Zheng, Lu Hou, Qiang Yao   +5 more
doaj   +4 more sources

A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens [PDF]

Shipin Kexue, 2023
With the improvement of people’s living standards, food safety has attracted more and more social attention. Organisms, especially microorganisms, are the most important factor that affects food safety.
WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei
doaj   +2 more sources

One-pot recombinase polymerase amplification and CRISPR/Cas12a assay for Cryptococcus neoformans [PDF]

Applied Microbiology and Biotechnology
Cry pto coc cus neoformans, an opportunistic fungal pathogen, can induce central nervous system infections, posing a life-threatening risk and imposing substantial global health challenges and economic burdens.
Zixin Li, Lei Chen, Jun Luo, Yamin Lu, Helin Zhang, Pingsen Zhao   +5 more
doaj   +2 more sources

Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay. [PDF]

PLoS Neglected Tropical Diseases, 2019
BackgroundAccess to an accurate diagnostic test for Buruli ulcer (BU) is a research priority according to the World Health Organization. Nucleic acid amplification of insertion sequence IS2404 by polymerase chain reaction (PCR) is the most sensitive and ...
Michael Frimpong, Hubert Senanu Ahor, Ahmed Abd El Wahed, Bernadette Agbavor, Francisca Naana Sarpong, Kenneth Laing, Mark Wansbrough-Jones, Richard Odame Phillips   +7 more
doaj   +2 more sources

One-step detection of Chlamydia psittaci using recombinase polymerase amplification and CRISPR/Cas12b [PDF]

AMB Express
The development of rapid, sensitive, and user-friendly diagnostic methods for Chlamydia psittaci (C. psittaci) is critical for early diagnosis and effective control of infections.
Jingyi Jiang, Jian Xu, Li Gong, Qiong Li, Ping Yao, Rong Wang, Jinyi Jiang, Lei Xu, Fengming Wang, Xujian Mao   +9 more
doaj   +2 more sources

Development of Reverse Transcriptase Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick for Rapid Detection of Tilapia Lake Virus (TiLV): Pilot Study [PDF]

Veterinary Sciences
Tilapia Lake Virus (TiLV) is well known as a highly contagious viral infection in aquaculture, particularly affecting Tilapia worldwide. Until recently, various TiLV diagnostic methods have been used for rapid and accurate diagnostic procedures that are ...
Saralee Srivorakul, Thunyamas Guntawang, Tidaratt Sittisak, Thanchanok Gordsueb, Kittikorn Boonsri, Rutch Khattiya, Nattawooti Sthitmatee, Kidsadagon Pringproa   +7 more
doaj   +2 more sources

Rapid and Sensitive Detection of the Causal Agents of Postharvest Kiwifruit Rot, Botryosphaeria dothidea and Diaporthe eres, Using a Recombinase Polymerase Amplification Assay [PDF]

The Plant Pathology Journal, 2023
The occurrence of postharvest kiwifruit rot has caused great economic losses in major kiwifruit-producing countries. Several pathogens are involved in kiwifruit rot, notably Botryosphaeria dothidea, and Diaporthe species.
Gi-Gyeong Park, Wonyong Kim, Kwang-Yeol Yang   +2 more
doaj   +1 more source

A novel cassette method for probe evaluation in the designed biochips [PDF]

, 2014
A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Al-humam, Abdulmohsen A., Alkhalil, Samia, Asatiani, Nino, Bogdarina, Irina, Kartvelishvili, Tamar, Mitchell, Julian, Sapojnikova, Nelly, Zinkevich, Vitaly   +7 more
core   +15 more sources