Results 1 to 10 of about 19,560 (116)

Recombinase polymerase amplification of forensic short tandem repeat loci [PDF]

Scientific Reports
Short tandem repeats (STRs) are highly polymorphic repetitive DNA sequences extensively used in forensic science for identification of individuals. STR genotyping is usually performed by capillary electrophoresis (CE) or next-generation sequencing (NGS ...
Sonja Škevin, Liesl De Keyzer, Lynn De Waele, Olivier Tytgat, David Van Hoofstat, Dieter Deforce, Filip Van Nieuwerburgh   +6 more
doaj   +2 more sources

Development of a rapid recombinase polymerase amplification assay for avian polyomaviruses [PDF]

Poultry Science
Budgerigar fledgling disease, caused by avian polyomavirus (APV), is an immunosuppressive condition in budgerigars that typically manifests as abdominal swelling, absence of down feathers on the dorsal and ventral regions, subdermal bleeding in nestlings,
Jiaqian Rao, Qian Wang, Mingji Li, Runlin Shao, Ruiheng Liu, Zhanxin Wang, Yang Liu, Jiajia Liu, Yuanjia Liu, Qingmei Xie, Xinheng Zhang   +10 more
doaj   +2 more sources

One-pot recombinase polymerase amplification and CRISPR/Cas12a assay for Cryptococcus neoformans [PDF]

Applied Microbiology and Biotechnology
Cry pto coc cus neoformans, an opportunistic fungal pathogen, can induce central nervous system infections, posing a life-threatening risk and imposing substantial global health challenges and economic burdens.
Zixin Li, Lei Chen, Jun Luo, Yamin Lu, Helin Zhang, Pingsen Zhao   +5 more
doaj   +2 more sources

One-step detection of Chlamydia psittaci using recombinase polymerase amplification and CRISPR/Cas12b [PDF]

AMB Express
The development of rapid, sensitive, and user-friendly diagnostic methods for Chlamydia psittaci (C. psittaci) is critical for early diagnosis and effective control of infections.
Jingyi Jiang, Jian Xu, Li Gong, Qiong Li, Ping Yao, Rong Wang, Jinyi Jiang, Lei Xu, Fengming Wang, Xujian Mao   +9 more
doaj   +2 more sources

Rapid and Sensitive Detection of the Causal Agents of Postharvest Kiwifruit Rot, Botryosphaeria dothidea and Diaporthe eres, Using a Recombinase Polymerase Amplification Assay [PDF]

The Plant Pathology Journal, 2023
The occurrence of postharvest kiwifruit rot has caused great economic losses in major kiwifruit-producing countries. Several pathogens are involved in kiwifruit rot, notably Botryosphaeria dothidea, and Diaporthe species.
Gi-Gyeong Park, Wonyong Kim, Kwang-Yeol Yang   +2 more
doaj   +1 more source

A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens [PDF]

Shipin Kexue, 2023
With the improvement of people’s living standards, food safety has attracted more and more social attention. Organisms, especially microorganisms, are the most important factor that affects food safety.
WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei
doaj   +1 more source

Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes

Frontiers in Bioengineering and Biotechnology, 2023
The rapid diagnosis of pathogenic infections plays a vital role in disease prevention, control, and public health safety. Recombinase-aided amplification (RAA) is an innovative isothermal nucleic acid amplification technology capable of fast DNA or RNA ...
Xiaoping Li, Xiaoping Li, Shuying Zhu, Xinling Zhang, Yanli Ren, Jing He, Jiawei Zhou, Liliang Yin, Gang Wang, Tian Zhong, Ling Wang, Ying Xiao, Ying Xiao, Chunying Zhu, Chengliang Yin, Xi Yu, Xi Yu   +16 more
doaj   +1 more source

Development of Recombinase Polymerase Amplification Combined with Lateral Flow Strips for Rapid Detection of Cowpea Mild Mottle Virus [PDF]

The Plant Pathology Journal, 2023
Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks.
Xinyang Wu, Shuting Chen, Zixin Zhang, Yihan Zhang, Pingmei Li, Xinyi Chen, Miaomiao Liu, Qian Lu, Zhongyi Li, Zhongyan Wei, Pei Xu   +10 more
doaj   +1 more source

Rapid detection of alveolar echinococcosis in hepatic nodules of horses by recombinase polymerase amplification assay

Veterinary and Animal Science, 2023
Alveolar echinococcosis in slaughtered horses remains a public health issue. This study aimed to develop a Recombinase polymerase amplification (RPA) assay targeting the mitochondrial NADH dehydrogenase subunit 5 (Nad5) gene of Echinococcus ...
Tatsuro Hifumi, Tetsuya Tanaka, Miho Sato, Kohei Akioka, Chiaki Fujimata, Noriaki Miyoshi   +5 more
doaj   +1 more source

A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci

Poultry Science, 2021
Chlamydia psittaci is a zoonotic agent of systemic wasting disease in birds and atypical pneumonia in mammalians including humans, constituting a public health risk. A rapid diagnostic assay would be beneficial in screening C. psittaci in the field.
Yanling Pang, Feng Cong, Xinheng Zhang, Hongxin Li, Yung-Fu Chang, Qingmei Xie, Wencheng Lin   +6 more
doaj   +1 more source