Results 41 to 50 of about 36,229 (210)

Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection [PDF]

, 2015
© 2015 Rosser et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in ...
A Ahmed, A. Rosser, AM Emery, B L Webster, BB Obeng, C Escadafal, C Shiff, CH King, D Rollinson, D Rollinson, D Wichmann, D. Rollinson, EF Kjetland, F Amarir, JR Stothard, JR Stothard, JS McCarthy, K Krolov, L Cnops, M. Forrest, MG Cavalcanti, MJ Werf van der, N Lodh, N Nausch, NP Pai, NV Vinkeles Melchers, OP Akinwale, R Bergquist, S Kersting   +28 more
core   +1 more source

Vibration mixing for enhanced paper-based recombinase polymerase amplification. [PDF]

Lab Chip
Isothermal nucleic acid amplification tests are a vital tool for point-of-care diagnostics. In this work, we significantly improve paper-based recombinase polymerase amplification of HIV-1 DNA and RNA by employing a vibration-based mixing platform.
Shimazu KN, Bender AT, Reinhall PG, Posner JD.   +3 more
europepmc   +3 more sources

Paradigm shift in the diagnosis of peste des petits ruminants: scoping review

Acta Veterinaria Scandinavica, 2020
Peste des petits ruminants virus causes a highly contagious disease, which poses enormous economic losses in domestic animals and threatens the conservation of wild herbivores.
Edson Kinimi, Steven Odongo, Serge Muyldermans, Richard Kock, Gerald Misinzo   +4 more
doaj   +1 more source

Rapid and specific detection of Yam mosaic virus by reverse-transcription recombinase polymerase amplification [PDF]

, 2015
Yam mosaic virus (YMV; genus Potyvirus) is considered to cause the most economically important viral disease of yams (Dioscorea spp.) in West Africa which is the dominant region for yam production globally.
Abarshi, Abd El Wahed, Aleman, Aleman-Verdaguer, Amer, Ampofo, Asala, Asiedu, Bousalem, Boyle, Chukwuemeka Nkere, Craw, Eni, Eni, Eni, Escadafal, Euler, Euler, Gonçalo Silva, Kenyon, Kenyon, Lillis, Mambole, Maroya, Mekuria, Menzel, Moritz Bömer, Mumford, Njukeng, Njukeng, Notomi, P. Lava Kumar, Piepenburg, Seal, Susan E. Seal, Teoh, Thouvenel, Xia, Zanoli, Zhang   +39 more
core   +1 more source

Evaluation of Isothermal Recombinase Polymerase Amplification Incubation Temperature Tolerance [PDF]

BioTechniques, 2016
doaj   +2 more sources

Equipment-free incubation of recombinase polymerase amplification reactions using body heat. [PDF]

PLoS ONE, 2014
The development of isothermal amplification platforms for nucleic acid detection has the potential to increase access to molecular diagnostics in low resource settings; however, simple, low-cost methods for heating samples are required to perform ...
Zachary Austin Crannell, Brittany Rohrman, Rebecca Richards-Kortum   +2 more
doaj   +1 more source

Assessment of pan-Leishmania detection by recombinase polymerase amplification assay

Diagnostic Microbiology and Infectious Disease, 2023
The spread of vector habitats along with increasing human mobility can introduce atypical Leishmania species and hence can challenge existing diagnostic practices for rapid detection of active infection with species outside the narrow target range. Here we assessed the pan-Leishmania detection ability of isothermal recombinase polymerase amplification (
Chiheb Louizi, Md Anik Ashfaq Khan, Khaledul Faisal, Rajashree Chowdhury, Prakash Ghosh, Faria Hossain, Thilini Nisansala, Shalindra Ranasinghe, Javier Moreno, Jorge Alvar, Dinesh Mondal, Timo Buhl, Carsten G.K. Lüder, Ahmed Abd El Wahed   +13 more
openaire   +3 more sources

Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification Assay

Pathogens, 2021
Feline coronavirus (FCoV) is endemic in cat populations worldwide. Persistently, subclinically infected cats play a significant role in spreading the infection.
Rea Maja Kobialka, Arianna Ceruti, Michelle Bergmann, Katrin Hartmann, Uwe Truyen, Ahmed Abd El Wahed   +5 more
doaj   +1 more source

RNAase III-Type Enzyme Dicer Regulates Mitochondrial Fatty Acid Oxidative Metabolism in Cardiac Mesenchymal Stem Cells [PDF]

, 2019
Cardiac mesenchymal stem cells (C-MSC) play a key role in maintaining normal cardiac function under physiological and pathological conditions. Glycolysis and mitochondrial oxidative phosphorylation predominately account for energy production in C-MSC ...
Jin, Yue, Kim, Il-man, Shen, Yan, Su, Xuan, Tang, Yaoliang, Weintraub, Neal L.   +5 more
core   +1 more source

An enhanced isothermal amplification assay for viral detection

Nature Communications, 2020
Current state-of-the-art diagnostics for infectious diseases are sensitive but require extensive equipment. Here the authors develop an enhanced recombinase polymerase amplification reaction for SARS-CoV-2 that allows for inexpensive and rapid testing ...
Jason Qian, Sarah A. Boswell, Christopher Chidley, Zhi-xiang Lu, Mary E. Pettit, Benjamin L. Gaudio, Jesse M. Fajnzylber, Ryan T. Ingram, Rebecca H. Ward, Jonathan Z. Li, Michael Springer   +10 more
doaj   +1 more source