Results 61 to 70 of about 36,229 (210)

Amplification Techniques of Recombinase & Polymerase and their Application in Parasite Detection [PDF]

E3S Web of Conferences, 2019
To study the application principles of recombinase polymerase amplification (RPA) and the specific situations of detecting parasites, the principles of RPA are analyzed to find the optimal temperature conditions, advantages, and disadvantages.
Niu Lili, Liu Hongli
doaj   +1 more source

A Nano‐Interception Strategy for Chronic Heart Failure: Prussian Blue Nanoparticles Disrupt Fibroblast‐Immune Communication via CCL2 Sequestration

Advanced Materials, EarlyView.
A nano‐interception strategy disrupts pathogenic fibroblast–macrophage crosstalk in chronic heart failure. Scalable Prussian blue nanoparticles selectively sequester CCL2 via ultrahigh‐affinity binding, preventing CCR2+ macrophage recruitment and breaking a key fibro‐inflammatory circuit. This approach demonstrates robust efficacy in murine and porcine
Bo Chen, Gao Wei, Guowei Zeng, Haoyu Li, Rongrong Wu, Hongyu Yan, Zijie Zhou, Kai Luo, Xiaoyang Zhang, Cong Li, Longming Huang, Lingyan Cheng, Xinjie Zhang, Jinghao Zheng, Bozhong Shi, Xiaojun Cai, Xiaomin He   +16 more
wiley   +1 more source

Recombinase polymerase amplification assay for rapid detection of Monkeypox virus [PDF]

Diagnostic Microbiology and Infectious Disease, 2019
In this study, a rapid method for the detection of Central and West Africa clades of Monkeypox virus (MPXV) using recombinase polymerase amplification (RPA) assay targeting the G2R gene was developed. MPXV, an Orthopoxvirus, is a zoonotic dsDNA virus, which is listed as a biothreat agent.
Davi, Saskia Dede, Kissenkötter, Jonas, Faye, Martin, Böhlken-Fascher, Susanne, Stahl-Hennig, Christiane, Faye, Oumar, Faye, Ousmane, Sall, Amadou A, Weidmann, Manfred, Ademowo, Olusegun George, Hufert, Frank T, Czerny, Claus-Peter, Abd El Wahed, Ahmed   +12 more
openaire   +4 more sources

Optimizing Illumina next-generation sequencing library preparation for extremely AT-biased genomes. [PDF]

, 2012
BAckground: Massively parallel sequencing technology is revolutionizing approaches to genomic and genetic research. Since its advent, the scale and efficiency of Next-Generation Sequencing (NGS) has rapidly improved. In spite of this success, sequencing
Campino, Susana, Gu, Yong, Kwiatkowski, Dominic P, Macinnis, Bronwyn, Manske, Magnus, Maslen, Gareth, Otto, Thomas D, Oyola, Samuel O, Quail, Michael A, Swerdlow, Harold P, Turner, Daniel J   +10 more
core  

Aurora-A expressing tumour cells are deficient for homology-directed DNA double strand-break repair and sensitive to PARP inhibition. [PDF]

, 2010
The protein kinase Aurora-A is a major regulator of the cell cycle that orchestrates mitotic entry and is required for the assembly of a functional mitotic spindle.
Afshan McCarthy, Alan Ashworth, Chan Tang, Christopher J. Lord, Despina Maniotis, Giet R, Goggins M, Gritsko TM, Spiros Linardopoulos, Tony Sourisseau   +9 more
core   +2 more sources

Reduced Maintenance DNA Methylation Thresholds Enable Sensitive Reporter Assays for UHRF1 and DNMT1 Inhibition

Advanced Science, EarlyView.
The authors engineer colorectal cancer cells with reduced redundancy of UHRF1 and DNMT1, key DNA methylation maintenance factors, lowering the maintenance threshold and sensitizing cells to inhibition. They develop reporter assays driven by endogenous tumor‐suppressor gene promoters with enhanced sensitivity and dynamic range to demethylating drugs ...
Cuicui Xia, Ying Cui, Jiongliang Wang, Wenjing Bai, Danyang Wang, Yao Chen, Huahui Guo, Liangyi Zong, Bing Qin, Xintong Cai, Jie Wang, Jing Huang, Scott B. Rothbart, Zufeng Guo, Stephen B. Baylin, Xiangqian Kong   +15 more
wiley   +1 more source

Magnetic concentration of allele-specific products from recombinase polymerase amplification [PDF]

Analytica Chimica Acta, 2019
The studied challenge is the specific detection of low-abundant genomic variants that differ by a single nucleotide from the wild type. The combination of blocked recombinase polymerase amplification (RPA) and selective capture by probes immobilised on magnetic-core particles integrated into a flow system is presented.
Sara Martorell, Luis A. Tortajada-Genaro, Ángel Maquieira   +2 more
openaire   +4 more sources

Recombinase polymerase amplification: method’s characteristics and applications in diagnostics of infectious diseases

Журнал микробиологии, эпидемиологии и иммунобиологии
Isothermal amplification techniques have been actively developed in recent years and are gradually introduced into the range of methods for infectious disease diagnostics. One of the fastest isothermal methods is recombinase polymerase amplification (RPA)
Olga S. Bondareva, Artem A. Baturin, Anna V. Mironova   +2 more
doaj   +1 more source

PSMA8‐Containing 20S Proteasome Regulates Spermiogenesis and Male Fertility

Advanced Science, EarlyView.
PSMA8 assembles s20S proteasome that degrades specific substrates in elongating spermatids. Degradation of s20S‐substrates activates translation of FXR1‐target mRNAs, which are essential for mitochondrial sheath formation and sperm morphogenesis.
Huiwen Cao, Qianting Zhang, Wei Xu, Anxuan Fang, Haomang Xu, Cheng Qiu, Lugeng He, Chao Yu   +7 more
wiley   +1 more source

Sensitive, Accurate and Rapid Detection of the Northern Root-Knot Nematode, Meloidogyne hapla, Using Recombinase Polymerase Amplification Assays

Plants, 2021
Rapid and reliable diagnostics of root-knot nematodes are critical for selections of effective control against these agricultural pests. In this study, recombinase polymerase amplification (RPA) assays were developed targeting the IGS rRNA gene of the ...
Sergei A. Subbotin, Julie Burbridge
doaj   +1 more source