Results 71 to 80 of about 19,050 (182)

Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay.

PLoS Neglected Tropical Diseases, 2019
BackgroundAccess to an accurate diagnostic test for Buruli ulcer (BU) is a research priority according to the World Health Organization. Nucleic acid amplification of insertion sequence IS2404 by polymerase chain reaction (PCR) is the most sensitive and ...
Michael Frimpong, Hubert Senanu Ahor, Ahmed Abd El Wahed, Bernadette Agbavor, Francisca Naana Sarpong, Kenneth Laing, Mark Wansbrough-Jones, Richard Odame Phillips   +7 more
doaj   +1 more source

Advances in large DNA fragment assembly for microbial cell factory engineering

Quantitative Biology, Volume 14, Issue 3, September 2026.
Abstract The efficient, rapid, and reliable assembly of DNA fragments is essential for advancing metabolic engineering and synthetic biology. With the rapid advancement of DNA synthesis and assembly technologies, the scale of DNA assembly has expanded from single genes to metabolic pathways and even genomes.
Yu Zhang, Wenqian Liu, Dongyuan Cheng, Jiazhang Lian, Xian Fu, Yue Shen   +5 more
wiley   +1 more source

Inducing Antigen‐Specific and Functional Immune Responses in Mice Toward Bovine Herpesvirus 1 and Bovine Respiratory Syncytial Virus by Chimeric Peptides Delivered by Bovine Herpesvirus 4‐Based Vector

Immunology, Volume 179, Issue 1, Page 54-71, September 2026.
Bovine Alphaherpesvirus 1 (BoHV‐1) and Bovine respiratory syncytial virus (BRSV) are major drivers of respiratory disease in cattle. (A) To target both pathogens, two chimeric antigens—membrane‐linked and secreted—combining BoHV‐1 glycoprotein D and BRSV fusion glycoprotein were designed and expressed using recombinant BoHV‐4‐based vectors.
Antonino Di Lorenzo, Sergio Minesso, Chiara Cossu, Valentina Franceschi, Elisabetta Bolli, Vittorio Madia, Laura Conti, Gaetano Donofrio   +7 more
wiley   +1 more source

Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants

Advanced Science, Volume 13, Issue 43, 3 August 2026.
A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei, Kai Zhang, Shuke Deng, Jinjie Chen, Xiaomei Huang, Jingfei Guo, Yuqing Wu, Yingjie Guo, Zhen Liang   +8 more
wiley   +1 more source

The RNA‐Binding Protein PARN Remodeled 3′ UTR Structure Defines Poly(A)‐Loading Sites to Mediate Immunoglobulin Homeostasis

Advanced Science, Volume 13, Issue 43, 3 August 2026.
ABSTRACT Class Switch Recombination (CSR) is essential for generating high‐affinity antibody isotypes from IgM during adaptive humoral responses. Despite well‐established roles for various transcription factors, whether CSR is subject to dedicated post‐transcriptional control represents a significant gap in knowledge.
Siyuan Sun, Chen Yang, Xiaoyu Wang, Naijing Hu, Shengyao Zhang, Xinchun Li, Yu Chen, Jianan Zhai, Xiangfeng Tang, Wei Lu, Chenxu Lu, Changchang Cao, Weiru Yu, Wenhao Wu, Xiaonan Nie, Fengchao Wang, Bing Fang, Fazheng Ren, Yixuan Li, Juan Chen   +19 more
wiley   +1 more source

Development of real-time recombinase polymerase amplification assay for rapid and sensitive detection of canine parvovirus 2

BMC Veterinary Research, 2017
Background Canine parvovirus 2, a linear single-stranded DNA virus belonging to the genus Parvovirus within the family Parvoviridae, is a highly contagious pathogen of domestic dogs and several wild canidae species.
Yunyun Geng, Jianchang Wang, Libing Liu, Yan Lu, Ke Tan, Yan-Zhong Chang   +5 more
doaj   +1 more source

IMACulate(DE3), an E. coli Strain for High Purity His‐Tagged Protein Purifications

Biotechnology and Bioengineering, Volume 123, Issue 8, Page 2127-2138, August 2026.
ABSTRACT Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his‐tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co‐purified with the target protein.
Jia Q. Truong, Dingyi Yu, Nan Hao, Chris Langendorf, Keith. E. Shearwin, Jessica K. Holien   +5 more
wiley   +1 more source

Rapid detection of mcr-1 by recombinase polymerase amplification

Journal of Medical Microbiology, 2018
The plasmid-mediated mcr-1 gene conferring colistin resistance has a strong ability to spread. The objective of this study was to establish a rapid and sensitive recombinase polymerase amplification (RPA) method for plasmid-mediated polymyxin-resistant gene mcr-1 detection.Using the reported sequence of the mcr-1 gene, we designed specific primers and ...
Jialiang, Xu, Xiaoxun, Wang, Liya, Yang, Biao, Kan, Xin, Lu   +4 more
openaire   +2 more sources

Generation and Characterization of a Rdh1‐iCre Line to Study Uterine Glandular Biology

genesis, Volume 64, Issue 4, August 2026.
ABSTRACT The uterus is an essential organ for fetal development in most mammals. Uterine glands, highly conserved structures in the mammalian uterus, play critical roles in the establishment and maintenance of pregnancy and have been implicated in the pathogenesis of uterine diseases, including endometrial cancer and endometriosis.
Mana Ohtomo, Shunsuke Takarabe, Takafumi Namiki, Yui Kawata, Ryosuke Kaneko, Manabu Ozawa, Yasuhiro Yamada, Hisashi Mori, Atsuko Kageyama, Maki Kamoshita, Jumpei Terakawa, Junya Ito   +11 more
wiley   +1 more source

Nanomaterial‐Based CRISPR/Cas‐Powered Electrochemical Sensing Integrated With Microfluidics for Rapid and Sensitive Diagnostics

Electrochemical Science Advances, Volume 6, Issue 4, August 2026.
ABSTRACT Rapid and sensitive detection of disease‐related biomarkers is critical for early diagnosis and appropriate treatment. However, conventional analytical methods are often time‐consuming and require complex procedures and skilled personnel.
Minju Ahn, Sang Baek Sim, Izzati Haizan, Jin‐Ha Choi, Jeong‐Woo Choi   +4 more
wiley   +1 more source