Results 71 to 80 of about 36,229 (210)

Application of recombinase polymerase amplification with lateral flow assay to pathogen point-of-care diagnosis. [PDF]

Front Cell Infect Microbiol
Zhao Y, Wei Y, Ye C, Cao J, Zhou X, Xie M, Qing J, Chen Z.   +7 more
europepmc   +2 more sources

FASLG Derived from Fibroblasts in Hydroxyapatite‐Rich Microenvironment Induces Urothelial Anoikis to Trigger Randall's Plaque Exposure

Advanced Science, EarlyView.
Randall's plaques (RP) serve as the nidus for calcium oxalate (CaOx) kidney stones. The current study reveals that hydroxyapatite (HAP) crystals activate the THY1–GSK3α/β–β‐catenin axis in renal interstitial fibroblasts (hRIFs), inducing FASLG secretion.
Minghui Liu, Maolan Wu, Meng Gao, Yongchao Li, Miao Yuan, Zhangcheng Liao, Zhi Liu, Yizhou Wang, Yu Cui, Jinbo Chen, Cheng He, Zhiyong Chen, Feng Zeng, Hequn Chen, Zewu Zhu   +14 more
wiley   +1 more source

Low-cost recombinase polymerase amplification (RPA) v1

, 2021
This protocol describes the expression of enzymes and creation of a master mix for recombinase polymerase amplification (RPA) assays.
openaire   +1 more source

Recombinase Polymerase Amplification (RPA) versus PCR for ancient DNA library amplification [PDF]

, 2019
Background : Recombinase Polymerase Amplification (RPA) is a relatively new isothermal methodology for amplifying DNA. RPA is similar to traditional PCR in that it produces an amplicon that is defined by the annealing of two opposing oligonucleotide primers.
Richards, Stephen M, Mitchell, Kieren J, Tobler, Raymond, Cooper, Alan   +3 more
openaire   +1 more source

Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis [PDF]

Journal of Clinical Microbiology, 2012
ABSTRACT Several real-time PCR approaches to develop field detection for Francisella tularensis , the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant ...
Euler, Milena, Wang, Yongjie, Otto, Peter, Tomaso, Herbert, Escudero, Raquel, Anda, Pedro, Hufert, Frank T, Weidmann, Manfred   +7 more
openaire   +4 more sources

Development of recombinase polymerase amplification detection method for Listeria monocytogenes in food

Zhongguo shipin weisheng zazhi, 2021
ObjectiveTo establish a recombinase polymerase amplification (RPA) method for the detection of Listeria monocytogenes (L. monocytogenes) in food.MethodsBased on the hlyA gene of L.
HE Jie, ZHU Chao, HUANG Shanshan, WU Qiaowei, SUN Ping, LAO Huajun   +5 more
doaj   +1 more source

A Single‐Enzyme Activated CRISPR‐Cas12a Nano System via Subtly Balanced dsDNA for Kinetic‐Gated UDG Detection and Spatiotemporal Cellular Imaging

Advanced Science, EarlyView.
Kinetic‐gated CRISPR–Cas12a activation enables single‐enzyme detection and spatiotemporal imaging of UDG ABSTRACT Uracil‐DNA glycosylase (UDG) is a key enzyme in base excision repair and an important biomarker for genomic stability and disease. In many reported sensing systems, uracil excision is coupled to signal generation through additional ...
Kejun Dong, Hao Hu, Haiyun Wang, Zijia Zheng, Shuangshuang Cheng, Wan Shu, Ruisi Liu, Xiaoyan Xin, Shiyi Huang, Dingchao Qian, Xianjin Xiao, Qiang Fu, Hongbo Wang   +12 more
wiley   +1 more source

TopCas: Topology‐Gated Cas12a via DNA‐RNA Chimeric Circular crRNA for Amplification‐Free Nucleic Acid Detection and Conditional Gene Editing

Advanced Science, EarlyView.
The TopCas platform utilizes an engineered DNA‐RNA chimeric circular crRNA to establish topology‐gated control over CRISPR‐Cas12a activity, enabling target‐triggered self‐catalytic amplification (preamplification‐free). This system seamlessly integrates highly sensitive molecular diagnostics with conditionally controlled gene editing, demonstrating ...
Shun Zhang, Wen Sun, Ting Xiao, Yali Wang, Xianlan Wu, Huiyou Chen, Ming Chen, Jun Zhang   +7 more
wiley   +1 more source

The CBRB regulon: Promoter dissection reveals novel insights into the CbrAB expression network in Pseudomonas putida [PDF]

, 2018
CbrAB is a high ranked global regulatory system exclusive of the Pseudomonads that responds to carbon limiting conditions. It has become necessary to define the particular regulon of CbrB and discriminate it from the downstream cascades through other ...
Andújar, Eloísa, Barroso, Rocío, Canosa, I., García Mauriño, Sofía M., Pérez Alegre, Mónica, Santero Santurino, Eduardo, Tomás Gallardo, Laura   +6 more
core   +3 more sources

SETD1A Regulates Glycolysis and Senescence of Nucleus Pulposus Cells via H3K4me3–HELZ2/PPARα‐HIF1α Axis to Drive Intervertebral Disc Degeneration

Advanced Science, EarlyView.
SETD1A is a key epigenetic regulator in NPCs during IDD. In normal NPCs, it sustains H3K4me3–HELZ2/PPARα–HIF1α signaling to maintain glycolytic energy metabolism and proliferation. In degenerated NPCs, reduced SETD1A disrupts this axis, impairing glycolysis and accelerating senescence, highlighting a promising therapeutic target for IDD.
Jiawei Fu, Xue Leng, Jiang Long, Zhengao Liao, Xiaoqin An, Xuezheng Ai, Dan Long, Changqing Li, Bo Huang, Yue Zhou, Shiwu Dong, Chencheng Feng   +11 more
wiley   +1 more source