The concentration of single-stranded DNA-binding proteins is a critical factor in recombinase polymerase amplification (RPA), as revealed by insights from an open-source system [PDF]
PeerJRecombinase polymerase amplification (RPA) facilitates rapid, exponential, isothermal nucleic acid amplification without the need for specialized equipment.
Francisco Cordoba-Andrade +17 more
doaj +3 more sources
Analytical and Clinical Assessment of a Portable, Isothermal Recombinase Polymerase Amplification (RPA) Assay for the Molecular Diagnosis of Urogenital Schistosomiasis [PDF]
Molecules, 2020 John Archer +2 more
exaly +2 more sources
Factors influencing Recombinase polymerase amplification (RPA) assay outcomes at point of care [PDF]
Molecular and Cellular Probes, 2016 Jason L Cantera +2 more
exaly +2 more sources
Development of Recombinase Polymerase Amplification Combined with Lateral Flow Strips for Rapid Detection of Cowpea Mild Mottle Virus [PDF]
The Plant Pathology Journal, 2023 Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks.
Xinyang Wu +10 more
doaj +1 more source
Frontiers in Cellular and Infection Microbiology, 2022 After the outbreak of SARS-CoV-2, nucleic acid testing quickly entered people’s lives. In addition to the polymerase chain reaction (PCR) which was commonly used in nucleic acid testing, isothermal amplification methods were also important nucleic acid ...
Meiying Tan +5 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2023 In the realm of pathogen detection, isothermal amplification technology has emerged as a swift, precise, and sensitive alternative to conventional PCR.
Xiaolei Mao +9 more
doaj +1 more source
Fish and Shellfish Immunology Reports, 2022 Elizabethkingia miricola is a highly infectious pathogen, which causes high mortality rate in frog farming. Therefore, it is urgent to develop a rapid and sensitive detection method.
Meihua Qiao +7 more
doaj +1 more source
A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens [PDF]
Shipin Kexue, 2023 With the improvement of people’s living standards, food safety has attracted more and more social attention. Organisms, especially microorganisms, are the most important factor that affects food safety.
WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei
doaj +1 more source
Frontiers in Marine Science, 2022 Enterospora artemiae, an obligate intracellular parasitic microsporidium, severely affects the development of Chinese grass shrimp (Palaemonetes sinensis) aquaculture. Currently, no effective drugs or vaccines are available for treatment.
Hongbo Jiang +8 more
doaj +1 more source
Frontiers in Plant Science, 2022 Establishing a portable diagnostic method for identifying plant pathogens is essential to prevent the spread of plant disease, especially in field and customs inspections. Leptosphaeria maculans (L.
Rong Lei +10 more
doaj +1 more source