Results 1 to 10 of about 2,915 (158)

Rapid SNP genotyping detection method based on PCR-lateral flow dipstick detection technique [PDF]

Scientific Reports
This study established a polymerase chain reaction–lateral flow dipstick (PCR-LFD) method for the visual detection of SNP genotypes. Targeting the MC4R gene SNP g.732 C > G, highly specific primers were designed for the mutation site, incorporating a ...
Junfang Jiang, Xiaowei Zhang, Pei Xiong, Xin Huang, Kaizhi Zheng, Yongqing Jiang, Sangang He, Huili Shan   +7 more
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Rapid detection of avian leukemia virus using CRISPR/Cas13a based lateral flow dipstick [PDF]

Frontiers in Veterinary Science
Avian leukemia virus (ALV) is one of the main pathogens of poultry tumor diseases, and has caused significant economic losses to the poultry industry since its discovery.
Jing Li, Zichuang Zhang, Zongshu Zhang, Xi Chen, Chunguang Wang, Xianghe Zhai, Tie Zhang   +6 more
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Detection of duck adenovirus 3 using RAA-CRISPR/Cas12a based lateral flow dipstick method [PDF]

Frontiers in Microbiology
Duck adenovirus 3 (DAdV-3) was firstly identified in Muscovy ducklings exhibiting slight pericardial effusion, swelling, and hemorrhaging in the kidneys, which is a poorly characterized duck virus.
Wenyu Zhang, Wenyu Zhang, Zhiwang Tang, Zhiwang Tang, Siwen He, Siwen He, Luoye Wang, Luoye Wang, Yongshan Zhou, Yongshan Zhou, Tingting Lu, Tingting Lu, Shuyu Chen, Shuyu Chen, Yu Huang, Chunhua Zhu, Chunhe Wan, Chunhe Wan, Wensong Jin, Jiayu Li   +19 more
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Development and Application of Fluorescent and Lateral Flow Dipstick Recombinase-Aided Amplification for Rapid Detection of Glaesserella parasuis [PDF]

Veterinary Sciences
Glaesserella parasuis (G. parasuis) is a bacterial respiratory pathogen from pigs, which can cause high morbidity and mortality in weaned piglets in piggery. An accurate and rapid detection for G.
Yongliang Che, Yao Wang, Renjie Wu, Longbai Wang, Xuemin Wu, Qiuyong Chen, Rujing Chen, Lunjiang Zhou   +7 more
doaj   +2 more sources

Development of Reverse Transcriptase Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick for Rapid Detection of Tilapia Lake Virus (TiLV): Pilot Study [PDF]

Veterinary Sciences
Tilapia Lake Virus (TiLV) is well known as a highly contagious viral infection in aquaculture, particularly affecting Tilapia worldwide. Until recently, various TiLV diagnostic methods have been used for rapid and accurate diagnostic procedures that are ...
Saralee Srivorakul, Thunyamas Guntawang, Tidaratt Sittisak, Thanchanok Gordsueb, Kittikorn Boonsri, Rutch Khattiya, Nattawooti Sthitmatee, Kidsadagon Pringproa   +7 more
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A Comprehensive Visual Detection Strategy: Versatile LAMP Assay with Phenol Red and Lateral Flow Dipstick for On-Site Detection of Riemerella anatipestifer [PDF]

Microorganisms
Riemerella anatipestifer (RA) is the primary causative agent of infectious serositis in ducks, causing significant economic losses. In this study, a rapid and visual loop-mediated isothermal amplification (LAMP) assay targeting the conserved region of ...
Jiafeng Wu, Nansong Jiang, Qizhang Liang, Hongmei Chen, Rongchang Liu, Qiuling Fu, Guanghua Fu, Chunhe Wan, Ping Wei, Longfei Cheng, Yu Huang, Tianchao Wei, Weiwei Wang   +12 more
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Rapid Detection of Peste Des Petits Ruminants via Multienzyme Isothermal and Lateral Flow Dipstick Combination Assay Based on N Gene [PDF]

Veterinary Sciences
In this study, a multienzyme isothermal and lateral flow dipstick combination assay for PPRV detection was established, the designed primers and probes targeting the N gene were screened and optimized, and analytical sensitivity, specificity, and ...
Jiamin Zhou, Jiao Xu, Jiani Li, Jiarong Yu, Yingli Wang, Jingyue Bao   +5 more
doaj   +2 more sources

Duplex Recombinase-Aided Amplification–Lateral Flow Dipstick (dRAA-LFD) Assay for New Zealand Green-Lipped Mussel Authentication [PDF]

Biosensors
New Zealand green-lipped mussel (Perna canaliculus) is a premium seafood product that may be substituted with morphologically similar mussels after shucking and cooking, particularly Asian green mussel (Perna viridis).
Jirakrit Saetang, Maturada Saengthong, Soottawat Benjakul, Gururaj Moorthy   +3 more
doaj   +2 more sources

Rapid visual detection of Staphylococcus epidermidis in bovine mastitis milk using LAMP–lateral flow dipstick [PDF]

Open Veterinary Journal
Background: The use of Loop-mediated Isothermal Amplification (LAMP) technology in combination with Lateral Flow Dipstick (LFD) for the rapid detection of Staphylococcus epidermidis (S.
Mingfeng Chu, Xiaoyu Chong, Wei Cheng, Huiying Zhang, Yuchen Liang, Mengke Si, Yiwei Wang, Junxue Qiu, Baolei Yang, Xuelong Chen, Yanping Qi, Haixia Wang   +11 more
doaj   +2 more sources

Development and evaluation of a recombinase-aided amplification-lateral flow dipstick assay for rapid and sensitive detection of Mycoplasma synoviae [PDF]

Frontiers in Cellular and Infection Microbiology
BackgroundMycoplasma synoviae (MS) is a critical avian pathogen responsible for enormous economic losses in global poultry production. Timely prevention and control of MS epidemics rely heavily on rapid, sensitive and accurate diagnostic approaches ...
Shu Wang, Yingkai Zhu, Yuxin Wang, Fang Wang, Kexun Lian, Xuemei Sun, Zexuan Wang, Xinghong Ding, Yali Han, Ruinan Wang, Yaocheng Cui, Huiyong Xuan   +11 more
doaj   +2 more sources