Results 31 to 40 of about 2,915 (158)

A novel rapid visual detection assay for Toxoplasma gondii combining recombinase-aided amplification and lateral flow dipstick coupled with CRISPR-Cas13a fluorescence (RAA-Cas13a-LFD)

Parasite, 2022
Toxoplasmosis, a parasitic disease resulting from Toxoplasma gondii infection, remains prevalent worldwide, and causes great harm to immunodepressed patients, pregnant women and newborns. Although various molecular approaches to detect T.
Zhao Jinhong, Li Yuanyuan, Xue Qiqi, Zhu Zhiwei, Zou Minghui, Fang Fang   +5 more
doaj   +1 more source

Rapid detection of H5 subtype avian influenza virus using CRISPR Cas13a based-lateral flow dipstick

Frontiers in Microbiology, 2023
Due to its high mortality rate, highly pathogenic avian influenza (HPAI), a notifiable animal illness designated by the World Organisation for Animal Health (WOAH), has caused enormous financial losses to the poultry sector.
Yang Li, Jiajing Shang, Jiajing Shang, Juan Luo, Juan Luo, Fuyou Zhang, Ge Meng, Ge Meng, Yingjie Feng, Yingjie Feng, Wenming Jiang, Xiaohui Yu, Chunran Deng, Chunran Deng, Guanhui Liu, Hualei Liu   +15 more
doaj   +1 more source

Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida

BMC Veterinary Research, 2019
Background Pasteurella multocida (P. multocida) is a bacterium that causes bovine respiratory disease (BRD) and haemorrhagic septicaemia (HS) in cattle, buffaloes and bison.
Guimin Zhao, Hongbin He, Hongmei Wang
doaj   +1 more source

Rapid visual detection of dengue virus by combining reverse transcription recombinase-aided amplification with lateral-flow dipstick assay

International Journal of Infectious Diseases, 2020
Objectives: Dengue caused by infection with the dengue virus (DENV) is endemic in the tropical and subtropical regions of the world and of greatest public health concern.
Yufeng Xiong, Yasha Luo, Huan Li, Weixiang Wu, Xiaolin Ruan, Xiaoping Mu   +5 more
doaj   +1 more source

Rapid detection assay for the invasive vase tunicate, Ciona intestinalis, using loop-mediated isothermal amplification combined with lateral flow dipstick [PDF]

Management of Biological Invasions, 2013
Invasive tunicate species threaten the shellfish aquaculture industry not only in Prince Edward Island (PEI) but also nationally andworldwide. Rapid screening tools for water samples are crucial for the efficient management of aquatic invasive species ...
Ahmed Siah, Patricia McKenna
doaj   +1 more source

Recombinase-aided amplification–lateral flow dipstick assay—a specific and sensitive method for visual detection of avian infectious laryngotracheitis virus

Poultry Science, 2021
The purpose of this study was to explore a specific, simple, and sensitive method for diagnosis of avian infectious laryngotracheitis virus. Recombinase-aided amplification (RAA) and lateral flow dipstick (LFD) were combined for labeling the optimized ...
Wenjing Wang, Chunguang Wang, Zichuang Zhang, Peng Zhang, Xianghe Zhai, Xinyue Li, Tie Zhang   +6 more
doaj   +1 more source

A rapid and visual detection assay for Senecavirus A based on recombinase-aided amplification and lateral flow dipstick. [PDF]

Front Cell Infect Microbiol
BackgroundSenecavirus A (SVA) is a newly pathogenic virus correlated with the acute death of piglets and vesicular lesions in pigs. The further prevalence of SVA will cause considerable economic damage to the global pig farming industry. Therefore, rapid and accurate diagnostic tools for SVA are crucial for preventing and controlling the disease ...
Song Y, Fang Y, Zhu S, Wang W, Wang L, Chen W, He Y, Yi L, Ding H, Zhao M, Fan S, Li Z, Chen J.   +12 more
europepmc   +4 more sources

Authenticity Identification of Meat Products Using Multienzyme Isothermal Rapid Amplification-Lateral Flow Dipstick [PDF]

Shipin Kexue
Purpose: To address the issue that laboratory methods cannot meet the need foron-site rapid testingat the grassroot level, a quick method for detecting the authenticity of meat products was established based on multienzyme isothermal rapid amplification (
SHANG Ke, ZHANG Biao, ZHANG min, YANG Huayu, YE Mei, DUAN Qingzi, WANG Wei, GONG Yajun
doaj   +1 more source

Rapid Multiplex Strip Test for the Detection of Circulating Tumor DNA Mutations for Liquid Biopsy Applications

Biosensors, 2022
In the era of personalized medicine, molecular profiling of patient tumors has become the standard practice, especially for patients with advanced disease.
Panagiota M. Kalligosfyri, Sofia Nikou, Sofia Karteri, Haralabos P. Kalofonos, Vasiliki Bravou, Despina P. Kalogianni   +5 more
doaj   +1 more source

Development of a lateral flow dipstick immunoassay for the rapid detection of glycyrrhizic acid

Food and Agricultural Immunology, 2006
Abstract A sensitive antibody-based lateral flow dipstick was developed for glycyrrhizic acid (GL) detection. The stick consisted of a sample pad, a conjugate pad, membrane and an absorbent pad. The membrane was coated with two capture reagents, GL-BSA conjugate and goat anti-mouse antibodies, forming a test line and a control line, respectively.
Jing Zhao Su-Ping He, Wei Liu, Ai-Xing Deng, Tie-Gui Nan, Bao-Min Wang, Zhi-Xi Zhai, Zhao-Hu Li   +6 more
openaire   +1 more source