Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 The rapid diagnosis of pathogenic infections plays a vital role in disease prevention, control, and public health safety. Recombinase-aided amplification (RAA) is an innovative isothermal nucleic acid amplification technology capable of fast DNA or RNA ...
Chengliang Yin, Yanli Ren, Liliang Yin
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Rapid detection of Mycoplasma hyopneumoniae by recombinase-aided amplification combined with the CRISPR/Cas12a system [PDF]
Frontiers in Cellular and Infection MicrobiologyMycoplasma hyopneumoniae (M. hyopneumoniae) is one of the primary agents involved in porcine respiratory disease complex, and circulates in the swine industry worldwide. The prevention and control of M. hyopneumoniae is complicated.
Hongyan Chen, Caixia Gao, Changyou Xia
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A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis [PDF]
Polish Journal of Microbiology, 2023 Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis. RAA can be performed in a closed tube at 39°C within 30 min.
Liu Jian +10 more
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Rapid detection of mpox virus using recombinase aided amplification assay [PDF]
Frontiers in Cellular and Infection Microbiology, 2023 A recent, unprecedented outbreak of human mpox virus infection has led to cases in non-African nations, and the number of confirmed or suspected cases outside of Africa has exceeded 1,000 within 5 weeks.
Xiaohu Cui +19 more
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Poultry Science, 2020 Newcastle disease is an acute and highly contagious disease of poultry caused by Newcastle disease virus infection, which does great harm to the poultry industry all over the world.
Tie Zhang, Wenjing Wang, Chunguang Wang
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Fast detection of duck circovirus by real-time fluorescence-based recombinase-aided amplification
Poultry Science, 2022 : Duck circovirus disease (DuCVD), as an immunosuppressive disease, is a threat to the poultry industry. In order to diagnose this disease quickly and accurately, a real-time fluorescence-based recombinase-aided amplification (RF-RAA) method was ...
Tie Zhang, Wenjing Wang, Zongshu Zhang
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Reverse Transcription Recombinase-Aided Amplification Assay for Newcastle Disease Virus in Poultry [PDF]
PathogensNewcastle disease (ND) is a highly contagious and economically significant viral infection that affects poultry globally, with recurrent outbreaks occurring even among vaccinated flocks in Egypt.
Nahed Yehia +8 more
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Detection of the mpox virus using a robust recombinase-aided amplification-based approach [PDF]
Biosafety and HealthIn 2022, a global outbreak of mpox was anticipated, with several cases reported in non-endemic countries in early May. Given the challenge of distinguishing the mpox virus (MPXV) from other pathogens based solely on symptoms, there is an urgent need for ...
Meihui Luo +22 more
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Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay
Life, 2023 Orf is an important zoonotic disease caused by the Orf virus (ORFV) which can cause contagious pustular dermatitis in goats and sheep. Orf is widespread in most sheep-raising countries in the world, causing huge economic losses.
Jiyu Guan, Zhendong Guo, Cheng Zhang
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Development of a real-time recombinase-aided amplification assay for rapid and sensitive detection of Edwardsiella piscicida [PDF]
Frontiers in Cellular and Infection MicrobiologyEdwardsiella piscicida, a significant intracellular pathogen, is widely distributed in aquatic environments and causes systemic infection in various species.
Yuchen Dong +4 more
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