Results 1 to 10 of about 2,883 (127)
Advanced Science, 2021 The Cas13a system has great potential in RNA interference and molecular diagnostic fields. However, lacking guidelines for crRNA design hinders practical applications of the Cas13a system in RNA editing and single nucleotide polymorphism identification ...
Behafarid Ghalandari +2 more
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CRISPR-Cas13a system: A novel tool for molecular diagnostics
Frontiers in Microbiology, 2022 The clustered regularly interspaced short palindromic repeats (CRISPR) system is a natural adaptive immune system of prokaryotes. The CRISPR-Cas system is currently divided into two classes and six types: types I, III, and IV in class 1 systems and types
Jintao Li, Lixin Zhao
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CRISPR/Cas13a: Compensatory Target Activation Mechanism [PDF]
Advanced ScienceCRISPR/Cas13a is a powerful RNA‐targeting platform for molecular diagnostics, but conventional single‐effector systems typically require contiguous RNA targets longer than ∼20–28 nt, limiting sensitivity and target flexibility.
Bowen Jiang +8 more
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Application of CRISPR/Cas13a system on the rapid detection of Salmonella spp. [PDF]
PLoS Neglected Tropical DiseasesBackgroundSalmonella spp. infections can manifest in various clinical symptoms, from asymptomatic carriage to gastroenteritis, and even severe sepsis.
Yongxin Huang +7 more
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The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells
Advanced Science, 2019 RNA is rarely used as a therapeutic target due to its flexible structure and instability. CRISPR‐Cas13a is a powerful tool for RNA knockdown, and the potential application of CRISPR‐Cas13a in cancer cells should be further studied.
Ye Wu, Chunsheng Kang, Qixue Wang
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Advanced Science, 2020 MicroRNAs (miRNAs) have been widely investigated as potential biomarkers for early clinical diagnosis of cancer. Developing an miRNA detection platform with high specificity, sensitivity, and exploitability is always necessary.
Da Xing, Mengqi Huang
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CRISPR anti-tag-mediated room-temperature RNA detection using CRISPR/Cas13a [PDF]
Nature CommunicationsThe CRISPR/Cas13a enzyme serves as a powerful tool for RNA detection due to its RNA-targeting capabilities. However, simple and highly sensitive detection using Cas13a faces challenges, such as the need for pre-amplification and elevated reaction ...
Jeong Moon +10 more
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On-site and visual detection of genotype VII Newcastle disease virus based on RT-RPA and CRISPR/Cas13a [PDF]
Frontiers in Veterinary ScienceIntroductionNewcastle Disease Virus (NDV) is not only a significant and persistent threat to the healthy development of poultry industry, but also an important pathogen endangering food safety and harboring potential zoonotic risks.MethodsIn this study ...
Yaping Huang +7 more
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Rapid, sensitive, and highly specific detection of Mycoplasma pneumoniae and its mutation using the RPA-CRISPR/Cas13a system [PDF]
Microbiology SpectrumMycoplasma pneumoniae (MP) is an important pathogen associated with respiratory tract infections. Macrolide-resistant MP infections (which mainly contain the A2063G mutation in their 23S rRNA) contribute to severe cases, complications, and sequelae ...
Yanli Ren +8 more
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Highly Sensitive Field Detection Technology for Anthrax Based on the CRISPR/Cas13a System [PDF]
Microbial BiotechnologyIn this study, we established a highly sensitive on‐site detection technology for Bacillus anthracis. Firstly, we integrated Multiple Enzyme Isothermal Rapid Amplification (MIRA) with the clustered regularly interspaced short palindromic repeats (CRISPR)
Jiao Fan +10 more
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