Results 11 to 20 of about 83,279 (184)
RNA virus interference via CRISPR/Cas13a system in plants [PDF]
Genome Biology, 2018 Background CRISPR/Cas systems confer immunity against invading nucleic acids and phages in bacteria and archaea. CRISPR/Cas13a (known previously as C2c2) is a class 2 type VI-A ribonuclease capable of targeting and cleaving single-stranded RNA (ssRNA ...
Rashid Aman +7 more
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Advances in application of CRISPR-Cas13a system [PDF]
Frontiers in Cellular and Infection MicrobiologyClustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) and CRISPR-associated (Cas) proteins serve as an adaptive immune system that safeguards prokaryotes and some of the viruses that infect prokaryotes from foreign nucleic acids (such as ...
Yue Zhang +7 more
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The CRISPR-Cas13a Gemini System for noncontiguous target RNA activation
Nature CommunicationsSimultaneous multi-target detection and multi-site gene editing are two key factors restricting the development of disease diagnostic and treatment technologies.
Hongrui Zhao +9 more
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Bacteriophage genome engineering with CRISPR–Cas13a [PDF]
Nature Microbiology, 2022 Jumbo phages such as Pseudomonas aeruginosa ФKZ have potential as antimicrobials and as a model for uncovering basic phage biology. Both pursuits are currently limited by a lack of genetic engineering tools due to a proteinaceous 'phage nucleus' structure that protects from DNA-targeting CRISPR-Cas tools. To provide reverse-genetics tools for DNA jumbo
Jingwen Guan +5 more
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Nucleic acid detection with CRISPR-Cas13a/C2c2 [PDF]
Science, 2017 Sensitive and specific CRISPR diagnostics Methods are needed that can easily detect nucleic acids that signal the presence of pathogens, even at very low levels. Gootenberg et al. combined the allele-specific sensing ability of CRISPR-Cas13a with recombinase polymerase ...
Jonathan S. Gootenberg +18 more
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CRISPR-Cas13a Inhibits HIV-1 Infection [PDF]
Molecular Therapy: Nucleic Acids, 2020 CRISPR-Cas provides bacteria and archaea with immunity against invading phages and foreign plasmid DNA and has been successfully adapted for gene editing in a variety of species.
Lijuan Yin +14 more
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A rapid and efficient platform for antiviral crRNA screening using CRISPR-Cas13a-based nucleic acid detection [PDF]
Frontiers in Immunology, 2023 IntroductionRapid and high-throughput screening of antiviral clustered regularly interspaced short palindromic repeat (CRISPR) RNAs (crRNAs) is urgently required for the CRISPR-Cas13a antiviral system.
Lan Yang +9 more
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Composition and Diversity of CRISPR-Cas13a Systems in the Genus Leptotrichia [PDF]
Frontiers in Microbiology, 2019 Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas13a, previously known as CRISPR-C2c2, is the most recently identified RNA-guided RNA-targeting CRISPR-Cas system that has the unique characteristics of both targeted and collateral ...
Shinya Watanabe +14 more
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Specific and sensitive detection of bovine coronavirus using CRISPR-Cas13a combined with RT-RAA technology [PDF]
Frontiers in Veterinary ScienceIntroductionBovine coronavirus (BCoV) is an important pathogen of enteric and respiratory disease in cattle, resulting in huge economic losses to the beef and dairy industries worldwide.
Zili Liang +6 more
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Rapid and Fully Microfluidic Ebola Virus Detection with CRISPR-Cas13a
ACS Sensors, 2019 Highly infectious illness caused by pathogens is endemic especially in developing nations where there is limited laboratory infrastructure and trained personnel. Rapid point-of-care (POC) serological assays with minimal sample manipulation and low cost are desired in clinical practice.
Peiwu Qin +10 more
openaire +3 more sources