Results 41 to 50 of about 83,279 (184)

Isothermal miRNA Detection via Ligation-Dependent CRISPR/Cas13a Activation. [PDF]

ACS Omega
Here, we present a rapid isothermal strategy for miRNA detection based on ligation-dependent generation of Cas13a-activating RNA and CRISPR/Cas13a-mediated signal amplification.
Lee J, Kim J, Jo DY, Lee S, Choi JK, Park HG.   +5 more
europepmc   +2 more sources

A Highly Sensitive and Specific Detection Method for Mycobacterium tuberculosis Fluoroquinolone Resistance Mutations Utilizing the CRISPR-Cas13a System

Frontiers in Microbiology, 2022
ObjectivesCRISPR-Cas13a system-based nucleic acid detection methods are reported to have rapid and sensitive DNA detection. However, the screening strategy for crRNAs that enables CRISPR-Cas13a single-base resolution DNA detection of human pathogens ...
Xiaopeng Bai, Panqi Gao, Keli Qian, Jiandong Yang, Haijun Deng, Tiwei Fu, Yuan Hu, Miaomiao Han, Huizhi Zheng, Xiaoxia Cao, Yuliang Liu, Yaoqin Lu, Ailong Huang, Quanxin Long   +13 more
doaj   +1 more source

Pathogen-Specific Bactericidal Method Mediated by Conjugative Delivery of CRISPR-Cas13a Targeting Bacterial Endogenous Transcripts

Microbiology Spectrum, 2022
The emergence of antibiotic-resistant bacteria threatens public health, and the use of broad-spectrum antibiotics often leads to unintended consequences, including disturbing the beneficial gut microbiota and resulting in secondary diseases.
Zihao Song, Yue Yu, Xinpeng Bai, Yiguo Jia, Jiayi Tian, Kui Gu, Mengyu Zhao, Changyu Zhou, Xiangyu Zhang, Hongning Wang, Yizhi Tang   +10 more
doaj   +1 more source

CRISPR-Cas13a system: a novel approach to precision oncology

Cancer Biology and Medicine, 2020
- Cite this article as: Zhang J, You Y. CRISPR-Cas13a system: a novel approach toprecision oncology. Cancer Biol Med. 2020; 17: 6-8.
Junxia Zhang, Yongping You
openaire   +4 more sources

CRISPR interference : a structural perspective [PDF]

, 2013
This article was made open access through BIS OA funding. The laboratory is funded by grants from the Biotechnology and Biological Sciences Research Council (BBSRC).CRISPR (cluster of regularly interspaced palindromic repeats) is a prokaryotic adaptive ...
Naismith, Jim, White, Malcolm F, Reeks, Judith Anne   +2 more
core   +1 more source

Universal theranostic CRISPR/Cas13a RNA-editing system for glioma

Theranostics, 2023
Background: The CRISPR/Cas13a system offers the advantages of rapidity, precision, high sensitivity, and programmability as a molecular diagnostic tool for critical illnesses. One of the salient features of CRISPR/Cas13a-based bioassays is its ability to recognize and cleave the target RNA specifically.
Wu, Ye, Wang, Yunfei, Zhou, Junhu, Wang, Jianhao, Zhan, Qi, Wang, Qixue, Yang, Eryan, Jin, Weili, Tong, Fei, Zhao, Jixing, Hong, Biao, Liu, Junrui, Kang, Chunsheng   +12 more
openaire   +2 more sources

Detection of Mitochondrial Mutations Through Isothermal Nucleic Acid Amplification Coupled With Clustered Regularly Interspaced Short Palindromic Repeat-Associated Endonuclease Cas13a

Frontiers in Genetics, 2021
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated endonuclease Cas13a can specifically bind and cleave RNA. After nucleic acid pre-amplification, bacterial Cas13a has been used to detect genetic mutations.
Hua Jiang, Kun Duan, Xu Han, Jun Wang, Xiao Liu, Maoxiao Yan, Yunxiu Wang, Hongyan Liu, Huiling Shi, Xiaoqing Gao, Chuan Ouyang, Xue Fu, Xinxin Zhang, Chao Liu   +13 more
doaj   +1 more source

N 1-Methyladenosine detection with CRISPR-Cas13a/C2c2

Chemical Science, 2019
CRISPR Cas13a provided a robust, simple and fluorescence-based method for m 1 A detection and dynamic m 1 A demethylation analysis of 28S rRNA.
Yi Chen, Shixi Yang, Shuang Peng, Wei Li, Fan Wu, Qian Yao, Fang Wang, Xiaocheng Weng, Xiang Zhou   +8 more
openaire   +3 more sources

Dual-gene detection in a single-tube system based on CRISPR-Cas12a/Cas13a for severe fever thrombocytopenia syndrome virus

Frontiers in Microbiology, 2022
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease, which is caused by severe fever with thrombocytopenia syndrome virus (SFTSV).
Yating Zhu, Yating Zhu, Chen Xing, Li Yang, Qian Li, Qian Li, Xiaofeng Wang, Xiaofeng Wang, Jing Zhou, Cong Zhang, Cuiping Ren, Cuiping Ren, Fahu Liu, Jun He, Bing Shen, Yinan Du, Yinan Du, Yan Liu   +17 more
doaj   +1 more source

Rapid and Sensitive Detection of Salmonella spp. Using CRISPR-Cas13a Combined With Recombinase Polymerase Amplification

Frontiers in Microbiology, 2021
Salmonella spp. is one of the most common foodborne disease-causing pathogens that can cause severe diseases in very low infectious doses. Rapid and sensitive detecting Salmonella spp. is advantageous to the control of its spread.
Bailin An, Bailin An, Hongbin Zhang, Xuan Su, Yue Guo, Tao Wu, Yiyue Ge, Fengcai Zhu, Lunbiao Cui, Lunbiao Cui   +9 more
doaj   +1 more source