Results 21 to 30 of about 83,279 (184)

Rapid and accurate detection method for bluetongue virus based on CRISPR-Cas13a combined with RT-ERA [PDF]

Frontiers in Cellular and Infection Microbiology
IntroductionBluetongue virus (BTV), a vector-borne pathogen of domestic and wild ruminants, poses substantial global threats to livestock health and trade.
Dong Zhou, Dong Zhou, Dong Zhou, Haibo Yu, Haibo Yu, Haibo Yu, Yuntong Shao, Yuntong Shao, Yuntong Shao, Caixia Gao, Caixia Gao, Caixia Gao, Changyou Xia, Changyou Xia, Changyou Xia, Yinglin Qi, Yinglin Qi, Yinglin Qi   +17 more
doaj   +2 more sources

CRISPR/Cas13a-based rapid detection method for porcine deltacoronavirus [PDF]

Frontiers in Microbiology
BackgroundPorcine deltacoronavirus (PDCoV) is a newly discovered porcine intestinal pathogenic coronavirus with a single-stranded positive-sense RNA genome and an envelope. PDCoV infects pigs of different ages and causes acute diarrhea and vomiting in newborn piglets.
Ran Luo, Zhimeng Cheng, Zhimeng Cheng, Haoyu Wang, Qiyue Yang, Yongping Zeng, Yijun Yang, Yuankun Chen, Wenting Li, Wenting Li, Xiao Liu, Xiao Liu   +11 more
openaire   +4 more sources

tRNA anticodon cleavage by target-activated CRISPR-Cas13a effector [PDF]

Science Advances
Type VI CRISPR-Cas systems are among the few CRISPR varieties that target exclusively RNA. The CRISPR RNA–guided, sequence-specific binding of target RNAs, such as phage transcripts, activates the type VI effector, Cas13. Once activated, Cas13 causes collateral RNA cleavage, which induces bacterial cell dormancy, thus protecting ...
Ishita Jain, Matvey Kolesnik, Konstantin Kuznedelov, Leonid Minakhin, Natalia Morozova, Anna Shiriaeva, Alexandr Kirillov, Sofia Medvedeva, Alexei Livenskyi, Laura Kazieva, Kira S. Makarova, Eugene V. Koonin, Sergei Borukhov, Konstantin Severinov, Ekaterina Semenova   +14 more
openaire   +4 more sources

Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics

Communications Biology
Cas12 and Cas13 are extensively utilized in molecular diagnostics for their trans-cleavage activities, yet their activation characteristics remain partially understood. Here, we conduct an in-depth investigation of Cas12a, Cas12f1, and Cas13a, uncovering
Qing Wu, Zhengfei Yi, Haoran Li, Guoxin Han, Jianyong Du, Jingwei Xiong, Keping Hu, Hai Gao   +7 more
doaj   +2 more sources

A CRISPR-Cas13a Based Strategy That Tracks and Degrades Toxic RNA in Myotonic Dystrophy Type 1

Frontiers in Genetics, 2020
Cas13a, an effector of type VI CRISPR-Cas systems, is an RNA guided RNase with multiplexing and therapeutic potential. This study employs the Leptotrichia shahii (Lsh) Cas13a and a repeat-based CRISPR RNA (crRNA) to track and eliminate toxic RNA ...
Nan Zhang, Brittani Bewick, Guangbin Xia, Denis Furling, Tetsuo Ashizawa   +4 more
doaj   +2 more sources

Discovery and Characterization of Small-Molecule Inhibitors of CRISPR/Cas13a

, 2021
Background: Clustered Regularly Interspaced Palindromic Repeats, or CRISPR, is an array of DNA sequences found in bacteria that confer immunity against phage. Sequences within the CRISPR array are transcribed into CRISPR RNA (crRNA), which hybridize with CRISPR associated proteins (Cas) to search for and cleave nucleic acids of the invading phage.
Reformat, Filip M
openaire   +2 more sources

Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and Nicotiana benthamiana

Viruses
Gene-editing technology, specifically the CRISPR-Cas13a system, has shown promise in breeding plants resistant to RNA viruses. This system targets RNA and, theoretically, can also combat RNA-based viroids.
Ying Wei Khoo, Qingsong Wang, Shangwu Liu, Binhui Zhan, Tengfei Xu, Wenxia Lv, Guangjing Liu, Shifang Li, Zhixiang Zhang   +8 more
doaj   +2 more sources

Rapid detection of avian leukemia virus using CRISPR/Cas13a based lateral flow dipstick [PDF]

Frontiers in Veterinary Science
Avian leukemia virus (ALV) is one of the main pathogens of poultry tumor diseases, and has caused significant economic losses to the poultry industry since its discovery.
Jing Li, Zichuang Zhang, Zongshu Zhang, Xi Chen, Chunguang Wang, Xianghe Zhai, Tie Zhang   +6 more
doaj   +2 more sources

Engineering Plant Immunity via CRISPR/Cas13a System

, 2018
Viral diseases constitute a major threat to the agricultural production and food security throughout the world. Plants cope with the invading viruses by triggering immune responses and small RNA interference (RNAi) systems. In prokaryotes, CRISPR/Cas systems function as an adaptive immune system to provide bacteria with resistance against invading ...
Aljedaani, Fatimah R.
openaire   +3 more sources

In Vitro Inhibition of Influenza Virus Using CRISPR/Cas13a in Chicken Cells [PDF]

Methods and Protocols, 2021
Advances in the field of CRISPR/Cas systems are expanding our ability to modulate cellular genomes and transcriptomes precisely and efficiently. Here, we assessed the Cas13a-mediated targeted disruption of RNA in chicken fibroblast DF1 cells.
Arjun Challagulla, Karel A. Schat, Timothy J. Doran   +2 more
doaj   +3 more sources