Results 1 to 10 of about 13,234 (117)
The effect of crRNA–target mismatches on cOA-mediated interference by a type III-A CRISPR-Cas system
RNA Biology, 2022 CRISPR systems elicit interference when a foreign nucleic acid is detected by its ability to base-pair to crRNA. Understanding what degree of complementarity between a foreign nucleic acid and crRNA is required for interference is a central question in ...
Jack A Dunkle +2 more
exaly +2 more sources
Advanced Science, 2021 The Cas13a system has great potential in RNA interference and molecular diagnostic fields. However, lacking guidelines for crRNA design hinders practical applications of the Cas13a system in RNA editing and single nucleotide polymorphism identification ...
Behafarid Ghalandari +2 more
exaly +2 more sources
Activation mechanism and integrated one-pot assay of chiral-like cRNA-enhanced CRISPR/Cas12a systems. [PDF]
Nat CommunThe 5′-repeat fragments released during pre-crRNA maturation are critical yet understudied components of the CRISPR/Cas12a system. Here, we demonstrate that engineered 5′-repeat fragments can potently activate Cas12a cleavage, with efficiency strongly ...
Li Y, Feng N, Wu X, Zhu B, Chen Y.
europepmc +2 more sources
Design of crRNA to Regulate MicroRNAs Related to Metastasis in Colorectal Cancer Using CRISPR-C2c2 (Cas13a) Technique [PDF]
Cell Journal, 2023 Colorectal cancer (CRC) is the third most prevalent cancer with the second-highest mortality rate worldwide. microRNAs(miRNAs) of cancer-derived exosomes have shown promising diagnosis potential.
Seyed Taleb Houseini +4 more
doaj +1 more source
In Vitro Inhibition of Influenza Virus Using CRISPR/Cas13a in Chicken Cells
Methods and Protocols, 2021 Advances in the field of CRISPR/Cas systems are expanding our ability to modulate cellular genomes and transcriptomes precisely and efficiently. Here, we assessed the Cas13a-mediated targeted disruption of RNA in chicken fibroblast DF1 cells.
Arjun Challagulla +2 more
doaj +1 more source
A combinatorial CRISPR-Cas12a attack on HIV DNA
Molecular Therapy: Methods & Clinical Development, 2022 CRISPR-Cas12a is an alternative class 2 gene editing tool that may cause less off-target effects than the original Cas9 system. We have previously demonstrated that Cas12a attack with a single CRISPR RNA (crRNA) can neutralize all infectious HIV in an ...
Minghui Fan +2 more
doaj +1 more source
CaSilico: A versatile CRISPR package for in silico CRISPR RNA designing for Cas12, Cas13, and Cas14
Frontiers in Bioengineering and Biotechnology, 2022 The efficiency of the CRISPR-Cas system is highly dependent on well-designed CRISPR RNA (crRNA). To facilitate the use of various types of CRISPR-Cas systems, there is a need for the development of computational tools to design crRNAs which cover ...
Adnan Asadbeigi +4 more
doaj +1 more source
Scientific Reports, 2023 The design of minimum CRISPR RNA (crRNA) sets for detection of diverse RNA targets using sequence degeneracy has not been systematically addressed. We tested candidate degenerate Cas13a crRNA sets designed for detection of diverse RNA targets (Lassa ...
T. A. Leski +6 more
doaj +1 more source
PLoS Biology, 2023 Cas12a is a programmable nuclease for adaptive immunity against invading nucleic acids in CRISPR-Cas systems. Here, we report the crystal structures of apo Cas12a from Lachnospiraceae bacterium MA2020 (Lb2) and the Lb2Cas12a+crRNA complex, as well as the
Li Jianwei +8 more
doaj +1 more source
Large scale screening of CRISPR guide RNAs using an optimized high throughput robotics system
Scientific Reports, 2022 All CRISPR/CAS systems utilize CRISPR guide RNAs (crRNAs), the design of which depend on the type of CAS protein, genetic target and the environment/matrix.
J. R. Spangler +4 more
doaj +1 more source