Establishment of RPA-CRISPR/Cas12a Detection Methods for Rapid Largemouth Bass Ranavirus Surveillance [PDF]
Veterinary SciencesThe high mortality associated with largemouth bass ranavirus (LMBRaV) disease requires rapid and sensitive diagnosis methods to prevent virus spread and subsequent outbreak. The recombinase polymerase amplification combined with the CRISPR/Cas12a system (
Haoyu Wang +11 more
doaj +2 more sources
Systematic Position Mapping of Split CRISPR-Cas12a Activators Enables Highly Sensitive miRNA Detection and Cancer Cell Stratification. [PDF]
Adv Sci (Weinh)ABSTRACT Amplification‐free Cas12a diagnostics with split crRNA enable rapid and programmable target recognition, yet insufficient understanding of DNA activator architecture prevents predictable control over trans‐cleavage activity and sensitivity. Here we systematically map over 200 split DNA activator configurations by introducing nicks at every ...
Tang X +8 more
europepmc +2 more sources
A Rapid and Sensitive CRISPR-Cas12a for the Detection of Legionella pneumophila [PDF]
Polish Journal of MicrobiologyLegionella pneumophila is a common environmental bacterium that can cause severe respiratory disease. In this study, a reliable, rapid, and convenient detection method for L.
LI JIANGHAO +7 more
doaj +2 more sources
Frontiers in Bioengineering and Biotechnology, 2022 Shigella flexneri is a serious threat to global public health, and a rapid detection method is urgently needed. The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system is widely used in gene editing, gene ...
Yaoqiang Shi +9 more
doaj +1 more source
Scientific Reports, 2022 The CRISPR-Cas system is widely used for genome editing of cultured cells and organisms. The discovery of a new single RNA-guided endonuclease, CRISPR-Cas12a, in addition to the conventional CRISPR-Cas9 has broadened the number of editable target sites ...
Riki Ishibashi +4 more
doaj +1 more source
Highly specific chimeric DNA-RNA-guided genome editing with enhanced CRISPR-Cas12a system
Molecular Therapy: Nucleic Acids, 2022 The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a system is composed of a Cas12a effector that acts as a DNA-cleaving endonuclease and a crispr RNA (crRNA) that guides the effector to the target DNA.
Hanseop Kim +18 more
doaj +1 more source
CRISPR/Cas12a Based Rapid Molecular Detection of Acute Hepatopancreatic Necrosis Disease in Shrimp
Frontiers in Veterinary Science, 2022 Acute hepatopancreatic necrosis disease (AHPND), formerly called early mortality syndrome (EMS), causes high mortality in cultured penaeid shrimp, particularly Penaeus vannamei and Penaeus monodon.
Chenglong Li +10 more
doaj +1 more source
Rapid detection of isocitrate dehydrogenase 1 mutation status in glioma based on Crispr-Cas12a
Scientific Reports, 2023 The aim is to use Crispr-Cas12a for the rapid detection of the single nucleotide polymorphism (SNP) of isocitrate dehydrogenase 1 (IDH1)-R132H locus and explore the effectiveness and consistency of this method with direct sequencing method for detecting ...
Zhebin Feng +8 more
doaj +1 more source
Sensitive Detection of KRAS Mutations by Clustered Regularly Interspaced Short Palindromic Repeats
Diagnostics, 2021 Kirsten rat sarcoma viral oncogene (KRAS) is the isoform most frequently mutated in human tumors. Testing for activating KRAS mutations has important implications for diagnosis and the personalized medicine of cancers.
Huifen Zhou +3 more
doaj +1 more source
Improving the efficiency of the CRISPR-Cas12a system with tRNA-crRNA arrays
Crop Journal, 2020 CRISPR-Cas12a offers a convenient tool for multiplex genome editing in rice. However, the CRISPR-Cas12a system displays variable editing efficiency across genomic loci, with marked influence by CRISPR RNAs (crRNAs).
Xixun Hu +4 more
doaj +1 more source