Results 1 to 10 of about 7,155 (145)

Cas12a chRDNA-mediated [PDF]

Molecular Therapy: Nucleic Acids
CRISPR hybrid guides containing a combination of RNA and DNA nucleotides (CRISPR hybrid RNA-DNA or chRDNA) enhance both Cas9 and Cas12a nuclease specificity and reduce off-target editing in vitro .
Marta Losa, Prachi Rajmane, Morena Stanaway, Mary Coons, Antonio Munoz, Vaibhavi Garish, Brandon Tan, Matt Thompson, Rachel Gonzalez, Glen Churchward, Arthur L.G. Owen, Jack Taylor, Zili An, Paul D. Donohoue, Scott Gradia, Meghdad Rahdar, Steven B. Kanner   +16 more
doaj   +2 more sources

Establishment of RPA-CRISPR/Cas12a Detection Methods for Rapid Largemouth Bass Ranavirus Surveillance [PDF]

Veterinary Sciences
The high mortality associated with largemouth bass ranavirus (LMBRaV) disease requires rapid and sensitive diagnosis methods to prevent virus spread and subsequent outbreak. The recombinase polymerase amplification combined with the CRISPR/Cas12a system (
Haoyu Wang, Yong Zhou, Peng Chen, Liping Zhang, Wen Zhu, Mingyang Xue, Yan Meng, Zhenyu Huang, Chen Xu, Yuding Fan, Chao Pei, Nan Jiang   +11 more
doaj   +2 more sources

Systematic Position Mapping of Split CRISPR-Cas12a Activators Enables Highly Sensitive miRNA Detection and Cancer Cell Stratification. [PDF]

Adv Sci (Weinh)
ABSTRACT Amplification‐free Cas12a diagnostics with split crRNA enable rapid and programmable target recognition, yet insufficient understanding of DNA activator architecture prevents predictable control over trans‐cleavage activity and sensitivity. Here we systematically map over 200 split DNA activator configurations by introducing nicks at every ...
Tang X, Li Z, Lu Y, Ma M, Mo Z, Ke J, Luan X, Luan T, Zhai J.   +8 more
europepmc   +2 more sources

Attomolar Detection of HIV-1 With Label-Free RCA-rCRISPR on Smartphone. [PDF]

Adv Sci (Weinh)
A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Mohammad N, Steksova A, Tang Y, Huang L, Velayati A, Zhang S, Dey Poonam A, Jamalzadegan S, Breen M, Chen H, Bagi M, Jiang G, Wei Q.   +12 more
europepmc   +2 more sources

Highly specific chimeric DNA-RNA-guided genome editing with enhanced CRISPR-Cas12a system

Molecular Therapy: Nucleic Acids, 2022
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a system is composed of a Cas12a effector that acts as a DNA-cleaving endonuclease and a crispr RNA (crRNA) that guides the effector to the target DNA.
Hanseop Kim, Wi-jae Lee, Chan Hyoung Kim, Yeounsun Oh, Lee Wha Gwon, Hyomin Lee, Woojeung Song, Junho K. Hur, Kyung-Seob Lim, Kang Jin Jeong, Ki-Hoan Nam, Young-Suk Won, Kyeong-Ryoon Lee, Youngjeon Lee, Young-Hyun Kim, Jae-Won Huh, Bong-Hyun Jun, Dong-Seok Lee, Seung Hwan Lee   +18 more
doaj   +1 more source

The trans DNA cleavage activity of Cas12a provides no detectable immunity against plasmid or phage

Frontiers in Genome Editing, 2022
Cas12a is a type V-A CRISPR-Cas RNA-guided endonuclease. It cleaves dsDNA at specific site, and then is activated for nonspecific ssDNA cleavage in trans in vitro. The immune function of the trans activity is still unknown.
Shunhang Liu, Xichen Rao, Xichen Rao, Ruiliang Zhao, Wenyuan Han   +4 more
doaj   +1 more source

Rapid and sensitive detection of Pseudomonas aeruginosa by isothermal amplification combined with Cas12a-mediated detection

Scientific Reports, 2023
CRISPR based technologies have been used for fast and sensitive detection of pathogens. To test the possibility of CRISPR based detection strategy in Pseudomonas aeruginosa infections, a combined method of recombinase polymerase amplification followed by
Siyi Huang, Xianfeng Wang, Xinchong Chen, Xiaoyu Liu, Qiuqing Xu, Lijun Zhang, Guangtao Huang, Jun Wu   +7 more
doaj   +1 more source

CRISPR/Cas12a Based Rapid Molecular Detection of Acute Hepatopancreatic Necrosis Disease in Shrimp

Frontiers in Veterinary Science, 2022
Acute hepatopancreatic necrosis disease (AHPND), formerly called early mortality syndrome (EMS), causes high mortality in cultured penaeid shrimp, particularly Penaeus vannamei and Penaeus monodon.
Chenglong Li, Nan Lin, Zhihua Feng, Minhua Lin, Biyun Guan, Kunsen Chen, Wangwang Liang, Qiaohuang Wang, Miaomiao Li, Yu You, Qi Chen   +10 more
doaj   +1 more source

Rapid detection of isocitrate dehydrogenase 1 mutation status in glioma based on Crispr-Cas12a

Scientific Reports, 2023
The aim is to use Crispr-Cas12a for the rapid detection of the single nucleotide polymorphism (SNP) of isocitrate dehydrogenase 1 (IDH1)-R132H locus and explore the effectiveness and consistency of this method with direct sequencing method for detecting ...
Zhebin Feng, Dongsheng Kong, Wei Jin, Kunyu He, Junyan Zhao, Bin Liu, Hanyun Xu, Xin’guang Yu, Shiyu Feng   +8 more
doaj   +1 more source

CRISPR/Cas12a-Enhanced Loop-Mediated Isothermal Amplification for the Visual Detection of Shigella flexneri

Frontiers in Bioengineering and Biotechnology, 2022
Shigella flexneri is a serious threat to global public health, and a rapid detection method is urgently needed. The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system is widely used in gene editing, gene ...
Yaoqiang Shi, Lan Kang, Rongrong Mu, Min Xu, Xiaoqiong Duan, Yujia Li, Chunhui Yang, Jia-Wei Ding, Qinghua Wang, Shilin Li   +9 more
doaj   +1 more source