Results 11 to 20 of about 7,205 (191)
Characterization of a thermostable Cas12a ortholog
Cell Insight, 2023 CRISPR-Cas12a has been used for genome editing and molecular diagnosis. The well-studied Cas12a orthologs have a T-rich PAM and are usually categorized as non-thermally stable enzymes.
Jing Wu +8 more
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Programmable RNA detection with CRISPR-Cas12a
Nature Communications, 2023 Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that multiple orthologs of Cas12a activate trans-cleavage in the presence of split
Santosh R. Rananaware +8 more
doaj +5 more sources
Recent advances of Cas12a applications in bacteria [PDF]
Applied Microbiology and Biotechnology, 2021 Abstract Clustered regularly interspaced short palindromic repeats (CRISPR)-mediated genome engineering and related technologies have revolutionized biotechnology over the last decade by enhancing the efficiency of sophisticated biological systems.
Meliawati Meliawati +2 more
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Broad-spectrum enzymatic inhibition of CRISPR-Cas12a [PDF]
Nature Structural & Molecular Biology, 2019 Cas12a is a bacterial RNA-guided nuclease used widely for genome editing and, more recently, as a molecular diagnostic. In bacteria, Cas12a enzymes can be inhibited by bacteriophage-derived proteins, anti-CRISPRs (Acrs), to thwart clustered regularly interspaced short palindromic repeat (CRISPR) adaptive immune systems.
Gavin J. Knott +6 more
openaire +4 more sources
Aptamer-Linked CRISPR/Cas12a-Based Immunoassay
Analytical Chemistry, 2021 The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas system has shown great promising applications in the area of nucleic acid biosensing. However, because of the dearth of versatile signal transduction strategies, this system is usually compromised to low versatility, moderate sensitivity, and complex operation for non-nucleic ...
Hui Li +5 more
openaire +3 more sources
BMC Biology, 2019 Background CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct features that have expanded genome editing capabilities. Cas12a-mediated genome editing is temperature sensitive in plants, but a lack of a comprehensive understanding ...
Aimee A. Malzahn +15 more
doaj +1 more source
Sensitive Detection of KRAS Mutations by Clustered Regularly Interspaced Short Palindromic Repeats
Diagnostics, 2021 Kirsten rat sarcoma viral oncogene (KRAS) is the isoform most frequently mutated in human tumors. Testing for activating KRAS mutations has important implications for diagnosis and the personalized medicine of cancers.
Huifen Zhou +3 more
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Parasites & Vectors, 2022 Background Infection with Trichomonas vaginalis can lead to cervicitis, urethritis, pelvic inflammatory disease, prostatitis and perinatal complications and increased risk of HIV transmission.
Shan Li +12 more
doaj +1 more source
Efficient Targeted Mutagenesis Mediated by CRISPR-Cas12a Ribonucleoprotein Complexes in Maize
Frontiers in Genome Editing, 2021 Recent advances in the development of CRISPR-Cas genome editing technologies have made it possible to perform targeted mutagenesis and precise gene replacement in crop plants. CRISPR-Cas9 and CRISPR-Cas12a are two main types of widely used genome editing
Shujie Dong +16 more
doaj +1 more source
Improved genome editing by an engineered CRISPR-Cas12a
Nucleic Acids Research, 2022 Abstract CRISPR-Cas12a is an RNA-guided, programmable genome editing enzyme found within bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single catalytic site to both cleave target double-stranded DNA (dsDNA) (cis-activity) and indiscriminately degrade single-stranded DNA (ssDNA) (trans-activity).
Enbo Ma +9 more
openaire +4 more sources