Results 21 to 30 of about 7,205 (191)
Improving the efficiency of the CRISPR-Cas12a system with tRNA-crRNA arrays
Crop Journal, 2020 CRISPR-Cas12a offers a convenient tool for multiplex genome editing in rice. However, the CRISPR-Cas12a system displays variable editing efficiency across genomic loci, with marked influence by CRISPR RNAs (crRNAs).
Xixun Hu +4 more
doaj +1 more source
Efficient generation of locus-specific human CAR-T cells with CRISPR/cCas12a
STAR Protocols, 2022 Summary: We recently developed a system to create human chimeric antigen receptor (CAR)-T cells using conjugated Cas12a (cCas12a) in which Cas12a is covalently linked to its CRISPR RNA (crRNA). This protocol describes site-specific modification of Cas12a
Xinyu Ling +3 more
doaj +1 more source
Frontiers in Microbiology, 2022 Mycoplasma hominis, which is difficult to culture and identify by ordinary methods, is one of the smallest pathogens in the human genitourinary tract causing urogenital infections.
Jialing Chen +16 more
doaj +1 more source
Dynamic Operations in Macromolecular Data Storage
Angewandte Chemie, EarlyView.With the exponential growth of digital data and the limitations of conventional silicon‐based storage and computing technologies, macromolecular data storage, the practice of encoding digital information within large, complex molecules like DNA or synthetic polymers, has emerged as a scalable and sustainable alternative to traditional digital ...
Jakub Ossowski +3 more
wiley +2 more sources
Angewandte Chemie, EarlyView.Two key binding events that collectively determine the catalytic efficiency of trans‐cleavage: (1) the substrate passing through the gap between the REC and NUC domains, determined by Kpass, and (2) the alignment of the substrate with the catalytic triad of the ribonucleoprotein, determined by Kalign.
Jianyu Hu +3 more
wiley +2 more sources
Characterization of Cme and Yme thermostable Cas12a orthologs
Communications Biology, 2022 AbstractCRISPR-Cas12a proteins are RNA-guided endonucleases that cleave invading DNA containing target sequences adjacent to protospacer adjacent motifs (PAM). Cas12a orthologs have been repurposed for genome editing in non-native organisms by reprogramming them with guide RNAs to target specific sites in genomic DNA. After single-turnover dsDNA target
Ryan T. Fuchs +6 more
openaire +3 more sources
The Versatile Tool: CRISPR/Cas12a System for Nucleic Acid Detection
Shengwu huaxue yu shengwu wuli jinzhanThe CRISPR/Cas system consists of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (Cas). The system forms an adaptive immune system in archaea and bacteria.
DANG Sheng, ZHANG Shuai, ZHAI Jing-Bo
doaj +1 more source
Multiplexed conditional genome editing with Cas12a in Drosophila [PDF]
Proceedings of the National Academy of Sciences, 2020 Abstract CRISPR-Cas genome engineering has revolutionised biomedical research by enabling targeted genome modification with unprecedented ease. In the popular model organism Drosophila melanogaster gene editing has so far relied exclusively on the prototypical CRISPR nuclease Cas9 ...
Port, Fillip +2 more
openaire +2 more sources
Scientific Reports, 2022 The CRISPR-Cas system is widely used for genome editing of cultured cells and organisms. The discovery of a new single RNA-guided endonuclease, CRISPR-Cas12a, in addition to the conventional CRISPR-Cas9 has broadened the number of editable target sites ...
Riki Ishibashi +4 more
doaj +1 more source
DNA Origami Post‐Processing by CRISPR‐Cas12a
Angewandte Chemie International Edition, 2020 AbstractCustomizable nanostructures built through the DNA‐origami technique hold tremendous promise in nanomaterial fabrication and biotechnology. Despite the cutting‐edge tools for DNA‐origami design and preparation, it remains challenging to separate structural components of an architecture built from—thus held together by—a continuous scaffold ...
Qiancheng Xiong +6 more
openaire +4 more sources