Results 41 to 50 of about 7,205 (191)
A combinatorial CRISPR-Cas12a attack on HIV DNA
Molecular Therapy - Methods & Clinical Development, 2022 CRISPR-Cas12a is an alternative class 2 gene editing tool that may cause less off-target effects than the original Cas9 system. We have previously demonstrated that Cas12a attack with a single CRISPR RNA (crRNA) can neutralize all infectious HIV in an infected T cell line in cell culture.
Minghui Fan +2 more
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Virology Journal, 2022 Background Integrating CRISPR-Cas12a sensors with isothermal signal amplification can be exploited to develop low-cost, disposable, and ultrasensitive assays for the diagnostics of human pathogens.
Weidong Qian +7 more
doaj +1 more source
Current Research in Food Science, 2022 With the large-scale planting of genetically modified (GM) crops, consumers were more aware of biosafety. Onsite rapid diagnostic methods were advantageous to the regulation of GM products.
Jinbin Wang +5 more
doaj +1 more source
Detection of Parasites in the Field: The Ever-Innovating CRISPR-Cas12a
Biosensors, 2023 The rapid and accurate detection of parasites is crucial for timely curative intervention in parasitosis and for epidemiological surveillance. To meet the needs of clinical diagnosis, it is imperative to develop a diagnostic tool based on nucleic acid that combines the sensitivity and specificity of established nucleic acid amplifica-tion tests with ...
Xin Li +7 more
openaire +3 more sources
Highly parallel profiling of the activities and specificities of Cas12a variants in human cells
Nature CommunicationsSeveral Cas12a variants have been developed to broaden its targeting range, improve the gene editing specificity or the efficiency. However, selecting the appropriate Cas12a among the many orthologs for a given target sequence remains difficult. Here, we
Peng Chen +9 more
doaj +1 more source
Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing
Animal Research and One Health, EarlyView.Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley +1 more source
Revolutionizing Healthcare With Paper‐Based Nucleic Acid Testing
Exploration, EarlyView.This work summarizes strategies to enhance paper‐based devices for nucleic acid testing. Key approaches include optimizing paper platforms, improving nucleic acid amplification, and refining labeling/signaling methods. These advancements aim to boost sensitivity, speed, and usability, making paper‐based diagnostics more effective for point‐of‐care ...
Hong Zhang +8 more
wiley +1 more source
Cas12a target search and cleavage on force stretched DNA
Biophysical Journal, 2021 CRISPR-Cas12a target search and cleavage on force-stretched λ -DNA using optical tweezers.
Losito, M. +4 more
openaire +4 more sources
Journal of NanobiotechnologyCRISPR-Cas12a system, characterized by its precise sequence recognition and cleavage activity, has emerged as a powerful and programmable tool for molecular diagnostics.
Lina Zhao +8 more
doaj +1 more source
CRISPR/Cas12a-Based Biosensing: Advances in Mechanisms and Applications for Nucleic Acid Detection
BiosensorsNucleic acid detection technology is crucial for molecular diagnosis. The advent of CRISPR/Cas12a-based nucleic acid detection has considerably broadened its scope, from the identification of infectious disease-causing microorganisms to the detection of ...
Kun Du +5 more
doaj +1 more source