Results 31 to 40 of about 7,205 (191)

CRISPR/Cas-based detection systems – emerging tools for plant pathology

Open Agriculture
Plant diseases pose a significant threat to global food security, causing substantial crop yield losses and economic damage. Traditional diagnostic methods often lack the speed, specificity, and sensitivity required for effective disease management.
Pozharskiy Alexandr, Kostyukova Valeriya, Adilbayeva Kamila, Taskuzhina Aisha, Gritsenko Dilyara   +4 more
doaj   +1 more source

A CRISPR Test for Detection of Circulating Nuclei Acids

Translational Oncology, 2019
Emerging CRISPR-based nucleic acid detection shows great promise in molecular diagnosis of diseases. CRISPR-Cas12a can sensitively and specifically detect human papillomavirus (HPV) DNA in anal swabs.
Jen-Hui Tsou, Qixin Leng, Feng Jiang
doaj   +1 more source

Enhanced Cas12a editing in mammalian cells and zebrafish

Nucleic Acids Research, 2019
Type V CRISPR-Cas12a systems provide an alternate nuclease platform to Cas9, with potential advantages for specific genome editing applications. Here we describe improvements to the Cas12a system that facilitate efficient targeted mutagenesis in mammalian cells and zebrafish embryos. We show that engineered variants of Cas12a with two different nuclear
Liu, Pengpeng, Luk, Kevin, Shin, Masahiro, Idrizi, Feston, Kwok, Samantha, Roscoe, Benjamin, Mintzer, Esther, Suresh, Sneha, Morrison, Kyle, Frazão, Josias B, Bolukbasi, Mehmet Fatih, Ponnienselvan, Karthikeyan, Luban, Jeremy, Zhu, Lihua Julie, Lawson, Nathan D, Wolfe, Scot A   +15 more
openaire   +3 more sources

Inhibition of CRISPR-Cas12a DNA targeting by nucleosomes and chromatin [PDF]

Science Advances, 2020
CRISPR-Cas12a cuts DNA in liquid-like chromatin condensates but is inhibited near nucleosomes.
Isabel Strohkendl, Fatema A. Saifuddin, Bryan A. Gibson, Michael K. Rosen, Rick Russell, Ilya J. Finkelstein   +5 more
openaire   +3 more sources

Transport–Reaction–Signal Coupling in Lateral Flow Assays for Next‐Generation Point‐of‐Care Diagnostics

Advanced Science, EarlyView.
Lateral flow assays are evolving into interdisciplinary, system‐level sensing platforms. Recent innovations are deconstructed through coupled phases of mass transfer, reaction, and signal transduction, revealing interdependence across porous materials, interfacial chemistry, nanolabels, readout devices, and data‐enabled analysis.
Yan Pan, Yuewei Li, Chao Mi, Zhengwen Yang, Lana McClements, Steven J. Langford, Jiayan Liao   +6 more
wiley   +1 more source

Rapid detection of feline parvovirus using RAA-CRISPR/Cas12a-based lateral flow strip and fluorescence

Frontiers in Microbiology
Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats, with high morbidity and mortality, necessitating a rapid and effective antigen diagnostic test with high sensitivity and specificity. In this study, a diagnostic platform based
Han Chen, Hailing Zhang, Jie Guo, Xiangshu Meng, Mengfan Yao, Longbin He, Xiaoxuan Nie, Han Xu, Chao Liu, Jian Sun, Jian Sun, Fei Wang, Yuelong Sun, Zhong Jiang, Yanliang He, Jianlou Zhang, Jianke Wang   +16 more
doaj   +1 more source

Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds

Genome Biology, 2019
CRISPR-Cas12a/Cpf1, a single RNA-guided endonuclease system, provides a promising tool for genome engineering. However, only three Cas12a orthologs have been employed for mammalian genome editing, and the editing efficiency as well as targeting coverage ...
Fei Teng, Jing Li, Tongtong Cui, Kai Xu, Lu Guo, Qingqin Gao, Guihai Feng, Chuanyuan Chen, Dali Han, Qi Zhou, Wei Li   +10 more
doaj   +1 more source

Co-freezing localized CRISPR-Cas12a system enables rapid and sensitive nucleic acid analysis

Journal of Nanobiotechnology
Rapid and sensitive nucleic acid detection is vital in disease diagnosis and therapeutic assessment. Herein, we propose a co-freezing localized CRISPR-Cas12a (CL-Cas12a) strategy for sensitive nucleic acid detection.
Lifeng Zhang, Shihua Luo, Wenbin Li, Wanting Su, Siting Chen, Chunchen Liu, Weilun Pan, Bo Situ, Lei Zheng, Ling Li, Xiaohui Yan, Ye Zhang   +11 more
doaj   +1 more source

MnO2 Nanosponge‐Accelerated Cas12a Trans‐Cleavage: Breaking the Kinetic Barrier for In Vivo RNA Imaging

Advanced Science
CRISPR/Cas12a system has emerged as a promising tool for in vitro biosensing, but its in vivo applications are hindered by its inefficient intracellular delivery and suboptimal trans‐cleavage kinetics.
Wen‐jing Liu, Lu‐yao Wang, Fei Ma, Chun‐yang Zhang   +3 more
doaj   +1 more source

Point-of-care testing of rpoB in Mycobacterium tuberculosis using multiply-primed-RCA coupled with CRISPR/Cas12a

Heliyon
Purpose: Due to the serious threat of tuberculosis to global health and limitations of existing diagnostic methods, this study combined the CRISPR/Cas12a system with Multiply-primed-RCA (MRCA) technology for Mycobacterium tuberculosis Point-of-care ...
Yingying Sun, Yaozhou Wu, Yulin Wang, Keke Li, Yanbin Chang, Lianhua Wei   +5 more
doaj   +1 more source