Results 51 to 60 of about 7,205 (191)

Borophene‐based sensing platforms: Pioneering ultrasensitive detection

FlexMat, EarlyView.
Schematic Diagram of Performance Optimization Strategies and Sensing Application Characteristics of Two‐Dimensional Boron‐Based Materials in Novel Sensors. Abstract Borophene is an emerging two‐dimensional (2D) material. Since significant breakthroughs were achieved in the experimental synthesis of borophene, the exploration and development of ...
Chi Yuan, Yanpeng Ji, Yi Liu, Guoan Tai
wiley   +1 more source

Systematic discovery of natural CRISPR-Cas12a inhibitors [PDF]

Science, 2018
Cas12 inhibitors join the anti-CRISPR family Bacteria and their phages continually coevolve in a molecular arms race. For example, phages use anti-CRISPR proteins to inhibit the bacterial type I and II CRISPR systems (see the Perspective by Koonin and Makarova). Watters et al. and Marino et al.
Kyle E. Watters, Christof Fellmann, Hua B. Bai, Shawn M. Ren, Jennifer A. Doudna   +4 more
openaire   +4 more sources

Efficient Genome Editing in Populus Using CRISPR/Cas12a

Frontiers in Plant Science, 2020
The ability to create targeted mutations using clustered regularly inter-spaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 in support of forest tree biotechnology is currently limited.
Yi An, Ya Geng, Junguang Yao, Chunxiang Fu, Mengzhu Lu, Chun Wang, Juan Du   +6 more
doaj   +1 more source

Engineered molecular diagnostic strategies for hepatocellular carcinoma: Integrating flexible photonic barcodes, liquid biopsies, and AI‐enhanced sensing platforms

FlexMat, EarlyView.
Abstract Early diagnosis of hepatocellular carcinoma (HCC) remains challenging because conventional serum biomarkers, especially alpha‐fetoprotein (AFP), show limited sensitivity for early‐stage disease. Recent progress in engineered molecular diagnostics provides new opportunities to improve HCC detection through integrated material, molecular, and ...
Jingzhi Xue, Wanqi Yang, Xiaoyu Liu, Xuehui Chu, Wei Li, Jinglin Wang   +5 more
wiley   +1 more source

Illuminating point‐of‐care diagnostics with aggregation‐induced emission biosensors

FlexMat, EarlyView.
This review highlights the application of aggregation‐induced emission luminogens (AIEgens) in point‐of‐care (POC) diagnostics for disease biomarker detection. It systematically examines a range of biosensing platforms, including lateral flow immunoassays, microfluidic chips, and electrochemiluminescence sensors, alongside their utility in detecting ...
Danni Hu, Yubing Hu, Dong Wang, Ben Zhong Tang   +3 more
wiley   +1 more source

Biosensors for Food Safety and Adulteration Monitoring: Applications, Smart Packaging Integration, and Pathways to Intelligent, Field‐Deployable Detection Systems

Food Safety and Health, EarlyView.
Biosensors enable rapid, sensitive, and portable detection of pathogens, contaminants, allergens, and food adulteration. Integration with smart packaging, smartphones, IoT, and machine learning supports real‐time monitoring and intelligent decision‐making, whereas microfluidics, biodegradable materials, explainable AI, and blockchain offer pathways ...
Mohima Akther Any, Abid Hassan Arnab, Liza Akter, Yusuf Chowdhury, Md. Hassan Bin Nabi, Md Abu Rayhan, Nazmul Islam, Wahidu Zzaman   +7 more
wiley   +1 more source

CRISPR/Cas12a Mediated Genome Editing Enhances Bombyx mori Resistance to BmNPV

Frontiers in Bioengineering and Biotechnology, 2020
CRISPR/Cas12a (Cpf1) is a single RNA-guided endonuclease that provides new opportunities for targeted genome engineering through the CRISPR/Cas9 system. Only AsCas12a has been developed for insect genome editing, and the novel Cas12a orthologs nucleases ...
Zhanqi Dong, Qi Qin, Zhigang Hu, Xinling Zhang, Jianghao Miao, Liang Huang, Peng Chen, Cheng Lu, Cheng Lu, Minhui Pan, Minhui Pan   +10 more
doaj   +1 more source

Frontiers in transition‐metal single‐atom electrocatalysts: Design tactics, mechanistic regulation, and electrochemical sensing technologies

InfoMat, EarlyView.
This review offers an overview of recent advances in the fundamental properties, synthesis methods, and structural optimization of transition‐metal single‐atom electrocatalysts for electrochemical sensing. It also provides a comprehensive summary of the emerging applications in electrochemical assays based on these materials, along with key challenges ...
Yue Cao, Bo‐Yu Chen, Yiru Sheng, Haoyuan Song, Linan Zhu, Xiaohan Yang, Yuehe Lin, Wenlei Zhu, Yang Zhou   +8 more
wiley   +1 more source

CRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA

Scientific Reports, 2022
CRISPR-Cas12a systems are becoming an attractive genome editing tool for cell engineering due to their broader editing capabilities compared to CRISPR-Cas9 counterparts.
D. J. Jedrzejczyk, L. D. Poulsen, M. Mohr, N. D. Damas, S. Schoffelen, A. Barghetti, R. Baumgartner, B. T. Weinert, T. Warnecke, R. T. Gill   +9 more
doaj   +1 more source

Ordered assembly of Cas12a for better performance of CRISPR/Cas system with application to the diagnosis of dental caries

Interdisciplinary Medicine, EarlyView.
Here, we present a tetrahedral DNA framework (TDF)‐mediated Cas12a ordered assembly strategy. TDFs arrange CRISPR/Cas12a into ordered structures controllably, enhancing catalytic efficiency through localized Cas12a hotspots while maintaining spatial intervals to facilitate substrate diffusion.
Yining Zhao, Panpan Qiao, Yujing Zeng, Murtaza Sayed, Qin Mi, Juan Zhang, Genxi Li   +6 more
wiley   +1 more source