Results 71 to 80 of about 7,205 (191)

CRISPR-Cas9/Cas12a biotechnology and application in bacteria

Synthetic and Systems Biotechnology, 2018
CRISPR-Cas technologies have greatly reshaped the biology field. In this review, we discuss the CRISPR-Cas with a particular focus on the associated technologies and applications of CRISPR-Cas9 and CRISPR-Cas12a, which have been most widely studied and used.
Yao, Ruilian, Liu, Di, Jia, Xiao, Zheng, Yuan, Liu, Wei, Xiao, Yi   +5 more
openaire   +5 more sources

A CRISPR‐Based Rapid Detection Assay for Crayfish Plague (Aphanomyces astaci) From Environmental Samples

Journal of Fish Diseases, EarlyView.
ABSTRACT Crayfish plague, caused by Aphanomyces astaci (Aa), is an infectious disease invasive in Europe, where its rapid spread has resulted in sharp declines of native crayfish species. Monitoring currently relies on a highly sensitive, but costly and time‐consuming qPCR approach.
Christopher Heine, Amadeus Plewnia, David Strand, Simran Gupta, Henrik Krehenwinkel, Gus R. McFarlane   +5 more
wiley   +1 more source

Programmable Domestication: CRISPR, Pan‐Genomics and System Level Engineering for Next‐Generation Crops

Plant Biotechnology Journal, EarlyView.
Conceptual overview of programmable de novo domestication from genetic diversity discovery to crop deployment. The framework connects diverse germplasm and genomic resources with three trajectory pathways. Adaptive Rescue, Agronomic Refinement and Novel Chassis defined by biological distance, engineering complexity and breeding objectives.
Muhammad Mubashar Zafar, Hina Firdous, Ayesha Siddiqua, Ayesha Naveed, Abdul Razzaq, Sadam Munawar, Aqsa Ijaz, Zunaira Anwar, Sezai Ercisli, Xuefei Jiang, Qiao Fei   +10 more
wiley   +1 more source

Clinical Laboratory Innovations: Nanoscale Detection of Chloramphenicol Using Nanomaterial Biosensors

Analytical Science Advances, Volume 7, Issue 2, December 2026.
Nanosensor schematic functionalized with specific bioreceptors that produce electrochemical signals upon Chloramphenicol binding. Resulting voltammetric readouts (differential pulse, square‐wave, and cyclic voltammetry) are analyzed to identify and quantify Chloramphenicol.
Nava Moghadasian Niaki, Reza Kheradmand, Seyedeh Giso Akbarian, Elham Shaterian, Hamed Shaterian, Ahmad Mobed   +5 more
wiley   +1 more source

Architecture of a DNA-guided Cas12a

Abstract CRISPR/Cas systems have largely been restricted to RNA-guided nucleases. Here, we present the cryo-EM structure of Acidaminococcus sp. Cas12a (AsCas12a) bound to a pseudo-DNA (ΨDNA) guide and RNA target, revealing how Cas12a accomplishes DNA-guided RNA recognition. The ΨDNA
Rodrigo Fregoso Ocampo, Carlos Orosco, Boyu Huang, Madeline S. West, Piyush K. Jain, David W. Taylor   +5 more
openaire   +2 more sources

A One-Pot CRISPR/Cas12a-Based Platform for Contamination-Free Nucleic Acid Amplification Detection

Biosensors
CRISPR-Cas12a enables rapid and specific detection of PCR/LAMP (loop-mediated isothermal amplification) reaction products; however, this approach often requires open-tube manipulation, rendering it prone to cross-contamination. Here, we developed a novel
Wei Tantai, Qinfeng Xu, Wenjuan Zhang, Yanni Li, Hao Liu   +4 more
doaj   +1 more source

CRISPR‐based therapeutic and modelling approaches in Huntington's disease: Progress, challenges and future directions

Clinical and Translational Discovery, Volume 6, Issue 5, October 2026.
Clustered regularly interspaced short palindromic repeat (CRISPR) is transforming Huntington's disease research through allele‐selective huntingtin (HTT) targeting, transcriptional and RNA suppression, and advanced disease modelling. Progress towards precision therapy depends on safe central nervous system (CNS) delivery, reduced off‐target and immune ...
Kairat Zhakipbekov, Surayyo Eshkabilova, Zokir Raximov, Sharof Eshonov, Abdusaid Khasanov, Ziyodulla Tokhtamurod, Axmadjan Ashurmetov, Avazbek Abduraxmanov, Niyozbek Abdurakhmonov, Elmurod Eshqobilov, Seyed Mohsen Mirhosseini, Amir Arsalan Ghahari   +11 more
wiley   +1 more source

Advances in CRISPR‐based detection technologies for severe fever with thrombocytopenia syndrome virus (Dabiebanda virus): A mini review

Clinical and Translational Discovery, Volume 6, Issue 5, October 2026.
This review summarises the detection methods for severe fever with thrombocytopenia syndrome virus based on CRISPR platforms. (A) CRISPR with fluorescent reporter. (B) CRISPR with lateral flow test strip. (C) CRISPR with DNAzyme. (D) Digital CRISPR. (E) CRISPR with single micro‐ring resonator biosensor. (F) self‐amplifying signal amplification strategy
Junhu Wang, Changqiang Zhu, Jinzhao Chu, Yingqing Mao, Yixin Ge, Haiming Yi, Hongming Wang, Yuexi Li, Yong Qi   +8 more
wiley   +1 more source

Engineering of a compact, high-fidelity EbCas12a variant that can be packaged with its crRNA into an all-in-one AAV vector delivery system.

PLoS Biology
The CRISPR-associated endonuclease Cas12a has become a powerful genome-editing tool in biomedical research due to its ease of use and low off-targeting. However, the size of Cas12a severely limits clinical applications such as adeno-associated virus (AAV)
Hongjian Wang, Jin Zhou, Jun Lei, Guosheng Mo, Yankang Wu, Huan Liu, Ziyan Pang, Mingkun Du, Zihao Zhou, Chonil Paek, Zaiqiao Sun, Yongshun Chen, Yan Wang, Peng Chen, Lei Yin   +14 more
doaj   +1 more source

A photocontrolled one-pot isothermal amplification and CRISPR-Cas12a assay for rapid detection of SARS-CoV-2 Omicron variants

Microbiology Spectrum
CRISPR-Cas technology has widely been applied to detect single-nucleotide mutation and is considered as the next generation of molecular diagnostics. We previously reported the combination of nucleic acid amplification (NAA) and CRISPR-Cas12a system to ...
Qian Sun, Hongqing Lin, Yuan Li, Liping Yuan, Baisheng Li, Yunan Ma, Haiying Wang, Xiaoling Deng, Hongliang Chen, Shixing Tang   +9 more
doaj   +1 more source