Results 81 to 90 of about 7,205 (191)

Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis

MicrobiologyOpen, Volume 15, Issue 5, October 2026.
This graphical abstract illustrates a rapid diagnostic platform for Mycoplasma bovis detection using Recombinase Polymerase Amplification coupled with CRISPR–Cas12a targeting the uvrC gene. The assay operates at 37°C–39°C, delivers results within 1 h, and demonstrates complete concordance with PCR in cattle and bison clinical samples.
Diba Yaghoubi, Viju V. Pillai
wiley   +1 more source

Multifunctional Hydrogels: Rational Design and Application in Regenerative Medicine

MedComm, Volume 7, Issue 10, October 2026.
Multifunctional hydrogels represent a paradigm shift in regenerative medicine, integrating rational material design with bioactive functionality to address complex tissue repair challenges. This review systematically examines the molecular engineering, structure–function relationships, and translational landscape of advanced hydrogel systems, spanning ...
Linyang Song, Epiphane K. Silli, Yazhen Xu, Huixia Lv, Ruoxian Song   +4 more
wiley   +1 more source

Targeting of CRISPR-Cas12a crRNAs into human mitochondria

Biochimie
Mitochondrial gene editing holds great promise as a therapeutic approach for mitochondrial diseases caused by mutations in the mitochondrial DNA (mtDNA). Current strategies focus on reducing mutant mtDNA heteroplasmy levels through targeted cleavage or base editing. However, the delivery of editing components into mitochondria remains a challenge. Here
Nikitchina, Natalia, Ulashchik, Egor, Shmanai, Vadim, Heckel, Anne-Marie, Tarassov, Ivan, Mazunin, Ilya, Entelis, Nina   +6 more
openaire   +3 more sources

CRISPR-Cas9/Cas12a systems for efficient genome editing and large genomic fragment deletions in Aspergillus niger

Frontiers in Bioengineering and Biotechnology
CRISPR technology has revolutionized fungal genetic engineering by accelerating the pace and expanding the feasible scope of experiments in this field. Among various CRISPR-Cas systems, Cas9 and Cas12a are widely used in genetic and metabolic engineering.
Guoliang Yuan, Guoliang Yuan, Shuang Deng, Shuang Deng, Jeffrey J. Czajka, Jeffrey J. Czajka, Ziyu Dai, Ziyu Dai, Beth A. Hofstad, Beth A. Hofstad, Joonhoon Kim, Joonhoon Kim, Kyle R. Pomraning, Kyle R. Pomraning   +13 more
doaj   +1 more source

Graft Biology in the CRISPR Era: From Tissue Fusion to Genome Compatibility

Plant-Environment Interactions, Volume 7, Issue 5, October 2026.
ABSTRACT Plant lineage has traditionally constrained grafting compatibility, with monocots generally considered incompatible because of their dispersed vascular bundles and limited secondary growth. Recent studies have shown that embryonic grafting can establish successful graft unions in selected monocot systems by exploiting early developmental ...
Melagani Subash Chandra Gowda Sahaja, Melagani Subash Chandra Gowda Sahana, Revathi Thottathil, Chithra Rekha, Poomangalath Priyada, Vidhu Sankar Babu   +5 more
wiley   +1 more source

Development of a Recombinase Polymerase Amplification and CRISPR-Cas12a-Based Assay for Rapid Detection of Rice Bakanae Disease Caused by Fusarium fujikuroi

Agronomy
Fusarium fujikuroi is the primary causal agent of rice bakanae disease, which can lead to substantial yield losses. Developing a rapid, highly specific, and accurate method for detecting F. fujikuroi is crucial for effective surveillance, prevention, and
Hongyu Li, Yue Qiu, Anpeng Zhang, Yingxiong Hu, Can Cheng, Jihua Zhou, Fuan Niu, Bin Sun, Yuting Dai, Kaizhen Xie, Zhizun Feng, Xiaorui Ding, Bilian Hu, Xueqing Zhang, Liming Cao, Huangwei Chu   +15 more
doaj   +1 more source

A dual gating mechanism controls target‐strand cleavage in Cas12j: Implications for engineering efficient nickases

Protein Science, Volume 35, Issue 10, October 2026.
Abstract The rapid expansion of CRISPR technologies has unveiled a diverse repertoire of RNA‐guided endonucleases, among them the compact Cas12j, which has emerged as a promising genome‐editing tool. Cas12j cleaves the two DNA strands sequentially; however, the molecular mechanism that regulates this cleavage remains incompletely understood.
Ilias G. Karvounis, Vangelis Daskalakis
wiley   +1 more source

A Robust Framework for Maize Elite Line Genome Editing Through Enhanced HI‐Edit via LbCas12a Activity Optimization

Plant Biotechnology Journal, Volume 24, Issue 10, Page 5392-5404, October 2026.
ABSTRACT Haploid induction coupled with genome editing (HI‐Edit) enables direct modification of commercial crop varieties, bypassing the need for trait introgression or direct transformation of elite lines with CRISPR machinery. However, its widespread application has been constrained by low haploid editing rates (HER), the proportion of haploids ...
Dawei Liang, Huanhuan Guo, Juan Wei, Fugui Zhu, Yuguo Zhang, Julie Green, Yun Ji, Huaibing Jin, Xiujuan Zhang, Huaping Gui, Hongmei Dan, Yubo Liu, Yu Zhang, Han Wang, Yutong Jiang, Lizhao Geng, Jian Lv, Wen Cai, Weibin Song, Timothy Kelliher, Xi Chen, Rachel Egger   +21 more
wiley   +1 more source

CRISPR-Cas12a–assisted PCR tagging of mammalian genes

Journal of Cell Biology, 2018
Here we describe a time-efficient strategy for endogenous C-terminal gene tagging in mammalian tissue culture cells. An online platform is used to design two long gene-specific oligonucleotides for PCR with generic template cassettes to create linear dsDNA donors, termed PCR cassettes.
Julia Fueller, Konrad Herbst, Matthias Meurer, Krisztina Gubicza, Bahtiyar Kurtulmus, Julia D. Knopf, Daniel Kirrmaier, Benjamin C. Buchmuller, Gislene Pereira, Marius K. Lemberg, Michael Knop   +10 more
openaire   +2 more sources

Generation of Allogeneic CAR‐T Circumvents Functional Deficits in Patient‐Derived Autologous Product for Glioblastoma

International Journal of Cancer, Volume 159, Issue 6, Page 1521-1534, 15 September 2026.
Clinical trials of chimeric antigen receptor T‐cell (CAR‐T) therapies in glioblastoma have shown limited clinical benefits. Whether this may be explained by the basal quality of CAR‐T products, which are currently generated using patient, autologous T‐cells, has been little explored.
Sabra K. Salim, Muhammad Vaseem Shaikh, Jeffrey Wei, William T. Maich, Alisha Anand, Oliver Y. Tang, Minomi K. Subapanditha, Zahra Alizada, Yujin Suk, Manoj Singh, Kui Zhai, Aapti Khanna, Benjamin Brakel, Vassil Dimitrov, Zoya Tabunshchyk, Katie Chan, Kevin R. Brown, Parvez Vora, Donald M. O'Rourke, Zev A. Binder, Chitra Venugopal, Jason Moffat, Sheila K. Singh   +22 more
wiley   +1 more source