Results 61 to 70 of about 7,205 (191)

Activated PI3K delta syndrome 1 mutations cause neutrophilia in zebrafish larvae

Disease Models & Mechanisms, 2023
Stone Elworthy, Holly A. Rutherford, Tomasz K. Prajsnar, Noémie M. Hamilton, Katja Vogt, Stephen A. Renshaw, Alison M. Condliffe   +6 more
doaj   +1 more source

Development of a new ERA-CRISPR/Cas12a method for rapid sensitive detection of Ralstonia pseudosolanacearum in eucalyptus

Frontiers in Plant Science
IntroductionRalstonia pseudosolanacearum is a significant pathogenic bacterium that causes bacterial wilt in Eucalyptus worldwide. Asymptomatic Eucalyptus cuttings may harbor substantial quantities of R.
Xiaoping Peng, Xiaoping Peng, Shengkun Wang, Yipin Zhang, Shengjie Wang, Sen Meng, Lipan Hu, Haibin Ma   +7 more
doaj   +1 more source

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

From knowledge to action: the importance of characterizing herbicide resistance mechanisms for implementing management strategies

Pest Management Science, EarlyView.
This review bridges the gap between understanding resistance mechanisms and applying them in weed management, highlighting how mechanistic insights reveal vulnerabilities that can guide more effective control strategies. Abstract Herbicide resistance remains one of the most intensively studied areas in weed science because of its direct consequences ...
Luan Cutti, Eric L. Patterson, Todd A. Gaines, Aldo Merotto   +3 more
wiley   +1 more source

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian, Hua Zhou, Zhenxing Zhao, Yongjiang Zhang, Wenjun Zhao, Lulu Cai, Tao Guo   +6 more
wiley   +1 more source

PAM-adjacent DNA flexibility tunes CRISPR-Cas12a off-target binding

Scientific Reports
Cas12a is a class 2 type V CRISPR-associated nuclease that uses an effector complex comprised of a single protein activated by a CRISPR-encoded small RNA to cleave double-stranded DNA at specific sites.
Aleique Allen, Brendon H. Cooper, Jaideep Singh, Remo Rohs, Peter Z. Qin   +4 more
doaj   +1 more source

Cell‐Based Micro/Nanorobots: From Living Carriers to Living Therapeutic Specialists

SmartBot, EarlyView.
Cell‐based micro/nanorobots integrate evolutionarily acquired biological intelligence with engineered capabilities, offering new opportunities for precision medicine in vivo. However, the complex physiological environments of different organs pose significant challenges to their clinical translation.
Junfeng Wu, Zhaoyu Zhang, Ke Xu, Enkuo Li, Yuting Cui, Yuting Zhou, Huanyu Zhou, Beipei Zhang, Shuai Jiang, Xiaodong Wang, Jialin Jiang, Lianqing Liu, Niandong Jiao   +12 more
wiley   +1 more source

Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi

Yeast, EarlyView.
Applications of transposon‐based tools for genome engineering in fungi include transposon‐mediated mutagenesis and targeted genomic integration. ABSTRACT Transposons are fundamental genetic elements that have profoundly shaped the architecture of eukaryotic genomes.
Bingyin Peng, Masahiro Tominaga, Chengqiang Wang, Robert E. Speight, Jun Ishii   +4 more
wiley   +1 more source

The discovery and development of Cas12A

Highlights in Science, Engineering and Technology
CRISPR-Cas12a stands as a transformative tool in genome editing, offering unparalleled precision and efficiency. Its utilization of an RNA-guided Cas12a enzyme enables targeted double-strand DNA breaks, allowing for the manipulation of genes with remarkable accuracy.
openaire   +1 more source

Cas12a and MAD7, genome editing tools for breeding

Breeding Science
Food shortages due to population growth and climate change are expected to occur in the near future as a problem that urgently requires solutions. Conventional breeding techniques, notably crossbreeding and mutation breeding, are known for being inefficient and time-consuming in obtaining seeds and seedlings with desired traits.
Hozumi, Shunya, Chen, Yi-Chen, Takemoto, Tatsuya, Sawatsubashi, Shun   +3 more
openaire   +2 more sources