Results 91 to 100 of about 7,205 (191)

Kinetic basis for Cas12a off-target discrimination

BMB Reports
CRISPR-Cas12a proteins are RNA-guided endonucleases classified as type V-A effectors that function similarly to Cas9, but possess distinct biochemical features. Previous studies have reported that compared to Cas9, Cas12a exhibits reduced off-target activity, yet the mechanistic origin of this high specificity remains unclear.
Yuyoung Kim, You Hee Choi, Minji Kim, Yunsu Jang, Sanghwa Lee   +4 more
openaire   +3 more sources

Development of a Copro-RPA-CRISPR/Cas12a assay to detect Echinococcus granulosus nucleic acids isolated from canine feces using NaOH-based DNA extraction method.

PLoS Neglected Tropical Diseases
BackgroundCystic echinococcosis (CE), caused by Echinococcus granulosus sensu lato (E. granulosus s.l.), remains a significant zoonotic parasitic disease affecting both livestock and humans.
Guoqing Shao, Xiaowei Zhu, Ruiqi Hua, Yanxin Chen, Guangyou Yang   +4 more
doaj   +1 more source

A Rapid and Sensitive CRISPR-Cas12a for the Detection of Legionella pneumophila

Polish Journal of Microbiology
Legionella pneumophila is a common environmental bacterium that can cause severe respiratory disease. In this study, a reliable, rapid, and convenient detection method for L.
LI JIANGHAO, WANG XINYU, WANG XINLING, QU HAI, GAO LIFEI, ZHAO ZHONGLING, LUO PEI, ZHENG YEHUAN   +7 more
doaj   +1 more source

Harnessing noncanonical crRNA for highly efficient genome editing

Nature Communications
The CRISPR-Cas12a system is more advantageous than the widely used CRISPR-Cas9 system in terms of specificity and multiplexibility. However, its on-target editing efficiency is typically much lower than that of the CRISPR-Cas9 system.
Guanhua Xun, Zhixin Zhu, Nilmani Singh, Jingxia Lu, Piyush K. Jain, Huimin Zhao   +5 more
doaj   +1 more source

CRISPR/Cas12a DTR system: a topology-guided Cas12a assay for specific dual detection of RNA and DNA targets

Nucleic Acids Research
Abstract The CRISPR/Cas12a technology has revolutionized molecular diagnostics. However, existing Cas12a systems depend on continuous target DNA activation, which limits them to single-target detection. In this study, we developed a novel topology-guided Cas12a system, the double-target responsive (DTR) system, capable of being activated
Qingyuan Jiang, Shuqi Jin, Zhichao Qin, Junqi Zhang, Ruyi He, Zhuo Chen, Bin Qiao, Jie Qiao, Yi Liu   +8 more
openaire   +2 more sources

Tailoring Cas12a functionality with a user-friendly and versatile crRNA variant toolbox

Nature Communications
Cas12a, with its unique targeting and cleavage activity towards DNA, has been widely applied in gene editing and molecular diagnostics. However, there currently lacks an activity regulation strategy that combines flexibility and simplicity to adapt ...
Jing Han, Yuan Min, Lan Hu, Jie-jie Chen, Shu-Xia Zhang, Xiao-Fan Li, Zhou-Hua Cheng, Dong-Feng Liu, Han-Qing Yu   +8 more
doaj   +1 more source

Rapid, sensitive, and user-friendly detection of Pseudomonas aeruginosa using the RPA/CRISPR/Cas12a system

BMC Infectious Diseases
Background Pseudomonas aeruginosa (P. aeruginosa) is a life-threatening bacterium known for its rapid development of antibiotic resistance, posing significant challenges in clinical treatment, biosecurity, food safety, and environmental monitoring. Early
Wenjing Zhang, Hai Qu, Xin Wu, Jingjing Shi, Xinling Wang   +4 more
doaj   +1 more source

Discovery of CRISPR-Cas12a clades using a large language model

Nature Communications
CRISPR-Cas systems revolutionize life science. Metagenomes contain millions of unknown Cas proteins. Traditional mining relies on protein sequence alignments.
Yuanyuan Feng, Junchao Shi, Zhanwei Li, Yongqian Li, Jiaxi Yang, Shisheng Huang, Jinfang Zheng, Wei Han, Yunbo Qiao, Jun Zhang, Qi Liu, Yao Yang, Chunyi Hu, Lina Wu, Xiaokang Zhang, Jin Tang, Xingxu Huang, Peixiang Ma   +17 more
doaj   +1 more source

Dynamic basis of supercoiling-dependent DNA interrogation by Cas12a via R-loop intermediates

Nature Communications
The sequence specificity and programmability of DNA binding and cleavage have enabled widespread applications of CRISPR-Cas12a in genetic engineering. As an RNA-guided CRISPR endonuclease, Cas12a engages a 20-base pair (bp) DNA segment by forming a three-
Kevin D. P. Aris, Joshua C. Cofsky, Honglue Shi, Noor Al-Sayyad, Ivan E. Ivanov, Ashwin Balaji, Jennifer A. Doudna, Zev Bryant   +7 more
doaj   +1 more source

CRISPR-Cas12a-based detection of monkeypox virus

Journal of Infection, 2022
Sui, Yutong, Xu, Qi, Liu, Mingsheng, Zuo, Kuiyang, Liu, Xiaomei, Liu, Jinyu   +5 more
openaire   +2 more sources