Results 11 to 20 of about 7,068 (190)
Programmable RNA detection with CRISPR-Cas12a
Nature Communications, 2023 Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that multiple orthologs of Cas12a activate trans-cleavage in the presence of split
Santosh R. Rananaware +8 more
doaj +5 more sources
CRISPR-Cas12a-Assisted Recombineering in Bacteria [PDF]
Applied and Environmental Microbiology, 2017 ABSTRACT Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a (Cpf1) has emerged as an effective genome editing tool in many organisms. Here, we developed and optimized a CRISPR-Cas12a-assisted recombineering system to facilitate genetic manipulation in bacteria.
Yan, Mei-Yi +5 more
openaire +2 more sources
Broad-spectrum enzymatic inhibition of CRISPR-Cas12a [PDF]
Nature Structural & Molecular Biology, 2019 Cas12a is a bacterial RNA-guided nuclease used widely for genome editing and, more recently, as a molecular diagnostic. In bacteria, Cas12a enzymes can be inhibited by bacteriophage-derived proteins, anti-CRISPRs (Acrs), to thwart clustered regularly interspaced short palindromic repeat (CRISPR) adaptive immune systems.
Gavin J. Knott +6 more
openaire +4 more sources
Improved genome editing by an engineered CRISPR-Cas12a
Nucleic Acids Research, 2022 Abstract CRISPR-Cas12a is an RNA-guided, programmable genome editing enzyme found within bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single catalytic site to both cleave target double-stranded DNA (dsDNA) (cis-activity) and indiscriminately degrade single-stranded DNA (ssDNA) (trans-activity).
Enbo Ma +9 more
openaire +4 more sources
CRISPR-Cas12a diagnostics for <i>acanthamoeba keratitis</i>. [PDF]
Ann Med Surg (Lond)Habib E, Hajj F.
europepmc +2 more sources
Aptamer-Linked CRISPR/Cas12a-Based Immunoassay
Analytical Chemistry, 2021 The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas system has shown great promising applications in the area of nucleic acid biosensing. However, because of the dearth of versatile signal transduction strategies, this system is usually compromised to low versatility, moderate sensitivity, and complex operation for non-nucleic ...
Hui Li +5 more
openaire +3 more sources
Inhibition of CRISPR-Cas12a DNA targeting by nucleosomes and chromatin [PDF]
Science Advances, 2020 CRISPR-Cas12a cuts DNA in liquid-like chromatin condensates but is inhibited near nucleosomes.
Isabel Strohkendl +5 more
openaire +3 more sources
Parasites & Vectors, 2022 Background Infection with Trichomonas vaginalis can lead to cervicitis, urethritis, pelvic inflammatory disease, prostatitis and perinatal complications and increased risk of HIV transmission.
Shan Li +12 more
doaj +1 more source
A combinatorial CRISPR-Cas12a attack on HIV DNA
Molecular Therapy - Methods & Clinical Development, 2022 CRISPR-Cas12a is an alternative class 2 gene editing tool that may cause less off-target effects than the original Cas9 system. We have previously demonstrated that Cas12a attack with a single CRISPR RNA (crRNA) can neutralize all infectious HIV in an infected T cell line in cell culture.
Minghui Fan +2 more
openaire +5 more sources
Detection of Parasites in the Field: The Ever-Innovating CRISPR-Cas12a
Biosensors, 2023 The rapid and accurate detection of parasites is crucial for timely curative intervention in parasitosis and for epidemiological surveillance. To meet the needs of clinical diagnosis, it is imperative to develop a diagnostic tool based on nucleic acid that combines the sensitivity and specificity of established nucleic acid amplifica-tion tests with ...
Xin Li +7 more
openaire +3 more sources