Results 21 to 30 of about 7,068 (190)

Mechanics of CRISPR-Cas12a and engineered variants on λ-DNA [PDF]

Nucleic Acids Research, 2021
Abstract Cas12a is an RNA-guided endonuclease that is emerging as a powerful genome-editing tool. Here, we selected a target site on bacteriophage λ-DNA and used optical tweezers combined with fluorescence to provide mechanistic insight into wild type Cas12a and three engineered variants, where the specific dsDNA and the unspecific ssDNA
Bijoya Paul, Loïc Chaubet, Dideke Emma Verver, Guillermo Montoya   +3 more
openaire   +4 more sources

DNA Origami Post‐Processing by CRISPR‐Cas12a

Angewandte Chemie International Edition, 2020
AbstractCustomizable nanostructures built through the DNA‐origami technique hold tremendous promise in nanomaterial fabrication and biotechnology. Despite the cutting‐edge tools for DNA‐origami design and preparation, it remains challenging to separate structural components of an architecture built from—thus held together by—a continuous scaffold ...
Qiancheng Xiong, Chun Xie, Zhao Zhang, Longfei Liu, John T Powell, Qi Shen, Chenxiang Lin   +6 more
openaire   +4 more sources

Systematic discovery of natural CRISPR-Cas12a inhibitors [PDF]

Science, 2018
Cas12 inhibitors join the anti-CRISPR family Bacteria and their phages continually coevolve in a molecular arms race. For example, phages use anti-CRISPR proteins to inhibit the bacterial type I and II CRISPR systems (see the Perspective by Koonin and Makarova). Watters et al. and Marino et al.
Kyle E. Watters, Christof Fellmann, Hua B. Bai, Shawn M. Ren, Jennifer A. Doudna   +4 more
openaire   +4 more sources

CRISPR-Cas12a-Assisted Genome Editing in Amycolatopsis mediterranei [PDF]

Frontiers in Bioengineering and Biotechnology, 2020
Amycolatopsis mediterranei U32 is an industrial producer of rifamycin SV, whose derivatives have long been the first-line antimycobacterial drugs. In order to perform genetic modification in this important industrial strain, a lot of efforts have been made in the past decades and a homologous recombination-based method was successfully developed in our
Yajuan Zhou, Yajuan Zhou, Yajuan Zhou, Xinqiang Liu, Xinqiang Liu, Jiacheng Wu, Jiacheng Wu, Jiacheng Wu, Guoping Zhao, Guoping Zhao, Jin Wang   +10 more
openaire   +3 more sources

Efficient Genome Editing in Populus Using CRISPR/Cas12a

Frontiers in Plant Science, 2020
The ability to create targeted mutations using clustered regularly inter-spaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 in support of forest tree biotechnology is currently limited.
Yi An, Ya Geng, Junguang Yao, Chunxiang Fu, Mengzhu Lu, Chun Wang, Juan Du   +6 more
doaj   +1 more source

Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools (Review)

Journal of Nanobiotechnology, 2022
Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods, soil or water can dramatically improve clinical outcomes and reduce the socioeconomic impact of diseases such as COVID-19.
Julija Dronina, Urte Samukaite-Bubniene, Arunas Ramanavicius   +2 more
doaj   +1 more source

An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a

Foods, 2023
CRISPR/Cas12a technology is used for nucleic acid detection due to its specific recognition function and non-specific single-stranded DNA cleavage activity.
Yuling Wang, Cheng Peng, Lin Ding, Zhixun Su, Xiaoyun Chen, Xiaofu Wang, Meihao Sun, Junfeng Xu   +7 more
doaj   +1 more source

Kinetic basis for DNA target specificity of CRISPR-Cas12a [PDF]

Molecular Cell, 2018
SUMMARY Class II CRISPR-Cas nucleases are programmable via a single guide RNA, enabling genome editing applications in nearly all organisms. However, DNA cleavage at off-target sites that resemble the target sequence is a pervasive problem that remains poorly understood mechanistically.
Strohkendl, Isabel, Saifuddin, Fatema A., Rybarski, James R., Finkelstein, Ilya J., Russell, Rick   +4 more
openaire   +2 more sources

A CRISPR Test for Detection of Circulating Nuclei Acids

Translational Oncology, 2019
Emerging CRISPR-based nucleic acid detection shows great promise in molecular diagnosis of diseases. CRISPR-Cas12a can sensitively and specifically detect human papillomavirus (HPV) DNA in anal swabs.
Jen-Hui Tsou, Qixin Leng, Feng Jiang
doaj   +1 more source

Current and Prospective Applications of CRISPR-Cas12a in Pluricellular Organisms

Molecular Biotechnology, 2022
AbstractCRISPR-Cas systems play a critical role in the prokaryotic adaptive immunity against mobile genetic elements, such as phages and foreign plasmids. In the last decade, Cas9 has been established as a powerful and versatile gene editing tool. In its wake, the novel RNA-guided endonuclease system CRISPR-Cas12a is transforming biological research ...
Shaheen Khan, Erwan Sallard
openaire   +2 more sources