Results 41 to 50 of about 7,068 (190)

Attomolar Detection of HIV‐1 With Label‐Free RCA‐rCRISPR on Smartphone

Advanced Science, EarlyView.
A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Noor Mohammad, Anastasiia Steksova, Yuyang Tang, Leyao Huang, Alireza Velayati, Shengwei Zhang, Aditi Dey Poonam, Sina Jamalzadegan, Matthew Breen, Hongjie Chen, Mahsa Bagi, Guochun Jiang, Qingshan Wei   +12 more
wiley   +1 more source

Structural insight into multistage inhibition of CRISPR-Cas12a by AcrVA4 [PDF]

Proceedings of the National Academy of Sciences, 2019
Prokaryotes possess CRISPR-Cas systems to exclude parasitic predators, such as phages and mobile genetic elements (MGEs). These predators, in turn, encode anti-CRISPR (Acr) proteins to evade the CRISPR-Cas immunity.
Ruchao Peng, Zhiteng Li, Ying Xu, Shaoshuai He, Qi Peng, Lian-ao Wu, Ying Wu, Jianxun Qi, Peiyi Wang, Yi Shi, George F. Gao   +10 more
openaire   +2 more sources

Unmodificated stepless regulation of CRISPR/Cas12a multi-performance

Nucleic Acids Research, 2023
Abstract As CRISPR technology is promoted to more fine-divided molecular biology applications, its inherent performance finds it increasingly difficult to cope with diverse needs in these different fields, and how to more accurately control the performance has become a key issue to develop CRISPR technology to a new stage.
Rong Zhao, Wang Luo, You Wu, Li Zhang, Xin Liu, Junjie Li, Yujun Yang, Li Wang, Luojia Wang, Xiaole Han, Zhongzhong Wang, Jianhong Zhang, Ke Lv, Tingmei Chen, Guoming Xie   +14 more
openaire   +2 more sources

Transport–Reaction–Signal Coupling in Lateral Flow Assays for Next‐Generation Point‐of‐Care Diagnostics

Advanced Science, EarlyView.
Lateral flow assays are evolving into interdisciplinary, system‐level sensing platforms. Recent innovations are deconstructed through coupled phases of mass transfer, reaction, and signal transduction, revealing interdependence across porous materials, interfacial chemistry, nanolabels, readout devices, and data‐enabled analysis.
Yan Pan, Yuewei Li, Chao Mi, Zhengwen Yang, Lana McClements, Steven J. Langford, Jiayan Liao   +6 more
wiley   +1 more source

Targeting of CRISPR-Cas12a crRNAs into human mitochondria

Biochimie
Mitochondrial gene editing holds great promise as a therapeutic approach for mitochondrial diseases caused by mutations in the mitochondrial DNA (mtDNA). Current strategies focus on reducing mutant mtDNA heteroplasmy levels through targeted cleavage or base editing. However, the delivery of editing components into mitochondria remains a challenge. Here
Nikitchina, Natalia, Ulashchik, Egor, Shmanai, Vadim, Heckel, Anne-Marie, Tarassov, Ivan, Mazunin, Ilya, Entelis, Nina   +6 more
openaire   +3 more sources

CRISPR-Cas12a–assisted PCR tagging of mammalian genes

Journal of Cell Biology, 2018
Here we describe a time-efficient strategy for endogenous C-terminal gene tagging in mammalian tissue culture cells. An online platform is used to design two long gene-specific oligonucleotides for PCR with generic template cassettes to create linear dsDNA donors, termed PCR cassettes.
Julia Fueller, Konrad Herbst, Matthias Meurer, Krisztina Gubicza, Bahtiyar Kurtulmus, Julia D. Knopf, Daniel Kirrmaier, Benjamin C. Buchmuller, Gislene Pereira, Marius K. Lemberg, Michael Knop   +10 more
openaire   +2 more sources

Development of a new ERA-CRISPR/Cas12a method for rapid sensitive detection of Ralstonia pseudosolanacearum in eucalyptus

Frontiers in Plant Science
IntroductionRalstonia pseudosolanacearum is a significant pathogenic bacterium that causes bacterial wilt in Eucalyptus worldwide. Asymptomatic Eucalyptus cuttings may harbor substantial quantities of R.
Xiaoping Peng, Xiaoping Peng, Shengkun Wang, Yipin Zhang, Shengjie Wang, Sen Meng, Lipan Hu, Haibin Ma   +7 more
doaj   +1 more source

Study Explains Specificity of CRISPR/Cas12a [PDF]

Cancer Discovery, 2018
Abstract A recent study reveals why the Cas12a genome-editing enzyme is more specific than the widely used Cas9. For Cas12a to cut, the guide RNA it carries must more closely match the sequence of the DNA target than with Cas9. The enzyme's specificity may make it a better choice for gene-editing applications.
openaire   +1 more source

Harnessing noncanonical crRNA for highly efficient genome editing

Nature Communications
The CRISPR-Cas12a system is more advantageous than the widely used CRISPR-Cas9 system in terms of specificity and multiplexibility. However, its on-target editing efficiency is typically much lower than that of the CRISPR-Cas9 system.
Guanhua Xun, Zhixin Zhu, Nilmani Singh, Jingxia Lu, Piyush K. Jain, Huimin Zhao   +5 more
doaj   +1 more source

A novel detection method for the pathogenic Aeromonas hydrophila expressing aerA gene and/or hlyA gene based on dualplex RAA and CRISPR/Cas12a

Frontiers in Microbiology, 2022
Aeromonas hydrophila is an emerging waterborne and foodborne pathogen with pathogenicity to humans and warm water fishes, which severely threatens human health, food safety and aquaculture.
Ziqin Lin, Jinfang Lu, Sihong Wu, Xi Lin, Laibao Zheng, Yongliang Lou, Xingxing Xiao   +6 more
doaj   +1 more source