Results 61 to 70 of about 7,068 (190)

A photocontrolled one-pot isothermal amplification and CRISPR-Cas12a assay for rapid detection of SARS-CoV-2 Omicron variants

Microbiology Spectrum
CRISPR-Cas technology has widely been applied to detect single-nucleotide mutation and is considered as the next generation of molecular diagnostics. We previously reported the combination of nucleic acid amplification (NAA) and CRISPR-Cas12a system to ...
Qian Sun, Hongqing Lin, Yuan Li, Liping Yuan, Baisheng Li, Yunan Ma, Haiying Wang, Xiaoling Deng, Hongliang Chen, Shixing Tang   +9 more
doaj   +1 more source

Biosensors for Food Safety and Adulteration Monitoring: Applications, Smart Packaging Integration, and Pathways to Intelligent, Field‐Deployable Detection Systems

Food Safety and Health, EarlyView.
Biosensors enable rapid, sensitive, and portable detection of pathogens, contaminants, allergens, and food adulteration. Integration with smart packaging, smartphones, IoT, and machine learning supports real‐time monitoring and intelligent decision‐making, whereas microfluidics, biodegradable materials, explainable AI, and blockchain offer pathways ...
Mohima Akther Any, Abid Hassan Arnab, Liza Akter, Yusuf Chowdhury, Md. Hassan Bin Nabi, Md Abu Rayhan, Nazmul Islam, Wahidu Zzaman   +7 more
wiley   +1 more source

The Versatile Tool: CRISPR/Cas12a System for Nucleic Acid Detection

Shengwu huaxue yu shengwu wuli jinzhan
The CRISPR/Cas system consists of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (Cas). The system forms an adaptive immune system in archaea and bacteria.
DANG Sheng, ZHANG Shuai, ZHAI Jing-Bo
doaj   +1 more source

Frontiers in transition‐metal single‐atom electrocatalysts: Design tactics, mechanistic regulation, and electrochemical sensing technologies

InfoMat, EarlyView.
This review offers an overview of recent advances in the fundamental properties, synthesis methods, and structural optimization of transition‐metal single‐atom electrocatalysts for electrochemical sensing. It also provides a comprehensive summary of the emerging applications in electrochemical assays based on these materials, along with key challenges ...
Yue Cao, Bo‐Yu Chen, Yiru Sheng, Haoyuan Song, Linan Zhu, Xiaohan Yang, Yuehe Lin, Wenlei Zhu, Yang Zhou   +8 more
wiley   +1 more source

MnO2 Nanosponge‐Accelerated Cas12a Trans‐Cleavage: Breaking the Kinetic Barrier for In Vivo RNA Imaging

Advanced Science
CRISPR/Cas12a system has emerged as a promising tool for in vitro biosensing, but its in vivo applications are hindered by its inefficient intracellular delivery and suboptimal trans‐cleavage kinetics.
Wen‐jing Liu, Lu‐yao Wang, Fei Ma, Chun‐yang Zhang   +3 more
doaj   +1 more source

Rapid detection of Mycoplasma hyopneumoniae by recombinase-aided amplification combined with the CRISPR/Cas12a system

Frontiers in Cellular and Infection Microbiology
Mycoplasma hyopneumoniae (M. hyopneumoniae) is one of the primary agents involved in porcine respiratory disease complex, and circulates in the swine industry worldwide. The prevention and control of M. hyopneumoniae is complicated.
Kaili Li, Kaili Li, Kaili Li, Tingyu Luo, Tingyu Luo, Tingyu Luo, Yu Zhang, Yu Zhang, Yu Zhang, Changwen Li, Changwen Li, Changwen Li, Hongyan Chen, Hongyan Chen, Hongyan Chen, Changyou Xia, Changyou Xia, Changyou Xia, Caixia Gao, Caixia Gao, Caixia Gao   +20 more
doaj   +1 more source

Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing

Rice, 2020
Background Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications.
Zhaohui Zhong, Shishi Liu, Xiaopei Liu, Binglin Liu, Xu Tang, Qiurong Ren, Jianping Zhou, Xuelian Zheng, Yiping Qi, Yong Zhang   +9 more
doaj   +1 more source

Ordered assembly of Cas12a for better performance of CRISPR/Cas system with application to the diagnosis of dental caries

Interdisciplinary Medicine, EarlyView.
Here, we present a tetrahedral DNA framework (TDF)‐mediated Cas12a ordered assembly strategy. TDFs arrange CRISPR/Cas12a into ordered structures controllably, enhancing catalytic efficiency through localized Cas12a hotspots while maintaining spatial intervals to facilitate substrate diffusion.
Yining Zhao, Panpan Qiao, Yujing Zeng, Murtaza Sayed, Qin Mi, Juan Zhang, Genxi Li   +6 more
wiley   +1 more source

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

Rapid detection of SARS-CoV-2 with CRISPR-Cas12a

PLOS Biology, 2020
The recent outbreak of betacoronavirus Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which is responsible for the Coronavirus Disease 2019 (COVID-19) global pandemic, has created great challenges in viral diagnosis. The existing methods for nucleic acid detection are of high sensitivity and specificity, but the need for complex sample ...
Dan Xiong, Wenjun Dai, Jiaojiao Gong, Guande Li, Nansong Liu, Wei Wu, Jiaqiang Pan, Chen Chen, Yingzhen Jiao, Huina Deng, Junwei Ye, Xuanxuan Zhang, Huiling Huang, Qianyun Li, Liang Xue, Xiuming Zhang, Guanghui Tang   +16 more
openaire   +4 more sources