Results 81 to 90 of about 7,068 (190)

CRISPR-Cas12a-based detection of monkeypox virus

Journal of Infection, 2022
Sui, Yutong, Xu, Qi, Liu, Mingsheng, Zuo, Kuiyang, Liu, Xiaomei, Liu, Jinyu   +5 more
openaire   +2 more sources

CRISPR/Cas12a with Universal crRNA for Indiscriminate Virus Detection

Molecules
Viruses, known for causing widespread biological harm and even extinction, pose significant challenges to public health. Virus detection is crucial for accurate disease diagnosis and preventing the spread of infections. Recently, the outstanding analytical performance of CRISPR/Cas biosensors has shown great potential and they have been considered as ...
Zhenlin Shang, Sitong Liu, Dongxu Liu, Xiaojing Pei, Shujing Li, Yifan He, Yigang Tong, Guoqi Liu   +7 more
openaire   +3 more sources

CRISPR-Cas9/Cas12a systems for efficient genome editing and large genomic fragment deletions in Aspergillus niger

Frontiers in Bioengineering and Biotechnology
CRISPR technology has revolutionized fungal genetic engineering by accelerating the pace and expanding the feasible scope of experiments in this field. Among various CRISPR-Cas systems, Cas9 and Cas12a are widely used in genetic and metabolic engineering.
Guoliang Yuan, Guoliang Yuan, Shuang Deng, Shuang Deng, Jeffrey J. Czajka, Jeffrey J. Czajka, Ziyu Dai, Ziyu Dai, Beth A. Hofstad, Beth A. Hofstad, Joonhoon Kim, Joonhoon Kim, Kyle R. Pomraning, Kyle R. Pomraning   +13 more
doaj   +1 more source

Advances in CRISPR‐based detection technologies for severe fever with thrombocytopenia syndrome virus (Dabiebanda virus): A mini review

Clinical and Translational Discovery, Volume 6, Issue 5, October 2026.
This review summarises the detection methods for severe fever with thrombocytopenia syndrome virus based on CRISPR platforms. (A) CRISPR with fluorescent reporter. (B) CRISPR with lateral flow test strip. (C) CRISPR with DNAzyme. (D) Digital CRISPR. (E) CRISPR with single micro‐ring resonator biosensor. (F) self‐amplifying signal amplification strategy
Junhu Wang, Changqiang Zhu, Jinzhao Chu, Yingqing Mao, Yixin Ge, Haiming Yi, Hongming Wang, Yuexi Li, Yong Qi   +8 more
wiley   +1 more source

Structural basis for AcrVA4 inhibition of specific CRISPR-Cas12a

eLife, 2019
CRISPR-Cas systems provide bacteria and archaea with programmable immunity against mobile genetic elements. Evolutionary pressure by CRISPR-Cas has driven bacteriophage to evolve small protein inhibitors, anti-CRISPRs (Acrs), that block Cas enzyme function by wide-ranging mechanisms.
Gavin J Knott, Brady F Cress, Jun-Jie Liu, Brittney W Thornton, Rachel J Lew, Basem Al-Shayeb, Daniel J Rosenberg, Michal Hammel, Benjamin A Adler, Marco J Lobba, Michael Xu, Adam P Arkin, Christof Fellmann, Jennifer A Doudna   +13 more
openaire   +5 more sources

Rapid and Simple Detection of Mycobacterium avium subsp. paratuberculosis Using a Lateral Flow Assay Based on CRISPR-Cas12a Combined with Recombinase Polymerase Amplification or Nested PCR

Pathogens
Paratuberculosis (PTB), caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic intestinal disease in ruminants. PTB is difficult to diagnose, control, and eradicate, leading to substantial economic losses.
Yue-Rong Lv, Yi-Yang Liu, Rong Zhang, Bo Yang, Shi-Yuan Xue, Yu-Lin Ding, Jun-Tao Jia, Hasi Bayaer, Alateng Bagen, Rui-Bin Chen, Siqin Tunala, Li Zhao, Yong-Hong Liu   +12 more
doaj   +1 more source

Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis

MicrobiologyOpen, Volume 15, Issue 5, October 2026.
This graphical abstract illustrates a rapid diagnostic platform for Mycoplasma bovis detection using Recombinase Polymerase Amplification coupled with CRISPR–Cas12a targeting the uvrC gene. The assay operates at 37°C–39°C, delivers results within 1 h, and demonstrates complete concordance with PCR in cattle and bison clinical samples.
Diba Yaghoubi, Viju V. Pillai
wiley   +1 more source

Rapid and visual detection of transmissible gastroenteritis virus using a CRISPR/Cas12a system combined with loop-mediated isothermal amplification

BMC Veterinary Research
Background Transmissible gastroenteritis (TGE) is a highly contagious intestinal disease caused by transmissible gastroenteritis virus (TGEV). The primary techniques for identifying TGEV involve enzyme-linked immunosorbent assay (ELISA), polymerase chain
Haiyang Wang, Zhao Qi, Jiale Wang, Zhenjie He, Liting Lu, Zhe Chen, Ying Shao, Guijun Wang, Zhenyu Wang, Jian Tu, Xiangjun Song   +10 more
doaj   +1 more source

Multifunctional Hydrogels: Rational Design and Application in Regenerative Medicine

MedComm, Volume 7, Issue 10, October 2026.
Multifunctional hydrogels represent a paradigm shift in regenerative medicine, integrating rational material design with bioactive functionality to address complex tissue repair challenges. This review systematically examines the molecular engineering, structure–function relationships, and translational landscape of advanced hydrogel systems, spanning ...
Linyang Song, Epiphane K. Silli, Yazhen Xu, Huixia Lv, Ruoxian Song   +4 more
wiley   +1 more source

A multifunctional switch for label-free CRISPR/Cas12a sensor with self-driven amplification

Synthetic and Systems Biotechnology
MicroRNA (miRNA) is promising candidate for non-invasive diagnostic biomarker. Conventional CRISPR/Cas12a-based miRNA detection systems are constrained by reliance on reverse transcription, nucleic acid pre-amplification and costly fluorescently labeled ...
Po Li, Xueying Lei, Xiaoying Niu, Wen Tian, Zhehuang Li, Songcheng Yu, Peng Zhang   +6 more
doaj   +1 more source