Results 91 to 100 of about 7,068 (190)
Graft Biology in the CRISPR Era: From Tissue Fusion to Genome Compatibility
Plant-Environment Interactions, Volume 7, Issue 5, October 2026.ABSTRACT Plant lineage has traditionally constrained grafting compatibility, with monocots generally considered incompatible because of their dispersed vascular bundles and limited secondary growth. Recent studies have shown that embryonic grafting can establish successful graft unions in selected monocot systems by exploiting early developmental ...
Melagani Subash Chandra Gowda Sahaja +5 more
wiley +1 more source
Journal of NanobiotechnologyCRISPR-Cas12a system, characterized by its precise sequence recognition and cleavage activity, has emerged as a powerful and programmable tool for molecular diagnostics.
Lina Zhao +8 more
doaj +1 more source
Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds
Genome Biology, 2019 CRISPR-Cas12a/Cpf1, a single RNA-guided endonuclease system, provides a promising tool for genome engineering. However, only three Cas12a orthologs have been employed for mammalian genome editing, and the editing efficiency as well as targeting coverage ...
Fei Teng +10 more
doaj +1 more source
Protein Science, Volume 35, Issue 10, October 2026.Abstract The rapid expansion of CRISPR technologies has unveiled a diverse repertoire of RNA‐guided endonucleases, among them the compact Cas12j, which has emerged as a promising genome‐editing tool. Cas12j cleaves the two DNA strands sequentially; however, the molecular mechanism that regulates this cleavage remains incompletely understood.
Ilias G. Karvounis, Vangelis Daskalakis
wiley +1 more source
Rapid and Visual Detection of the tet(X4) Gene Using LAMP-CRISPR/Cas12a and RPA-CRISPR/Cas12a
The increasing prevalence of the plasmid-mediated tigecycline resistance gene tet(X4) has severely limited clinical treatment options. Isothermal amplification techniques, which do not require thermal cycling equipment, are designed for rapid clinical detection.
Yao Shen +10 more
openaire +1 more source
Plant Biotechnology Journal, Volume 24, Issue 10, Page 5392-5404, October 2026.ABSTRACT Haploid induction coupled with genome editing (HI‐Edit) enables direct modification of commercial crop varieties, bypassing the need for trait introgression or direct transformation of elite lines with CRISPR machinery. However, its widespread application has been constrained by low haploid editing rates (HER), the proportion of haploids ...
Dawei Liang +21 more
wiley +1 more source
International Journal of Cancer, Volume 159, Issue 6, Page 1521-1534, 15 September 2026.Clinical trials of chimeric antigen receptor T‐cell (CAR‐T) therapies in glioblastoma have shown limited clinical benefits. Whether this may be explained by the basal quality of CAR‐T products, which are currently generated using patient, autologous T‐cells, has been little explored.
Sabra K. Salim +22 more
wiley +1 more source
CRISPR-Cas12a-assisted nucleic acid detection [PDF]
Cell Discovery, 2018 Shi-Yuan Li +8 more
openaire +2 more sources
HeliyonBackground: The development of a robust and accurate point-of-care platform for the detection of tuberculosis (TB) biomarkers is important for disease control.
Saman Taufiq +3 more
doaj +1 more source
Microbiology SpectrumSugarcane yellow leaf virus (SCYLV) can reduce sugarcane productivity. A novel detection system based on reverse transcription-multienzyme isothermal rapid amplification (RT-MIRA) combined with CRISPR-Cas12a, named RT-MIRA-CRISPR-Cas12a, was developed ...
Ting Wang +8 more
doaj +1 more source