Results 101 to 110 of about 7,068 (190)

A dual fluorescence channel RAA-based CRISPR-Cas12a/Cas13a system for highly sensitive detection of Gyrovirus galga1 and Gyrovirus homsa1

Virulence
Gyrovirus galga1 (GyG1) and Gyrovirus homsa1 (GyH1) are the second and third most common gyroviruses identified, respectively, after chicken anaemia virus. They were first reported in 2011 and are currently prevalent worldwide.
Dan Yu, Zhixun Xie, Yanfang Zhang, Zhiqin Xie, Qing Fan, Sisi Luo, Liji Xie, Meng Li, Tingting Zeng, Minxiu Zhang, Xiaofeng Li, You Wei, Aiqiong Wu, Lijun Wan   +13 more
doaj   +1 more source

CRISPR-Cas12a exhibits metal-dependent specificity switching

Nucleic Acids Research
Abstract Cas12a is the immune effector of type V-A CRISPR-Cas systems and has been co-opted for genome editing and other biotechnology tools. The specificity of Cas12a has been the subject of extensive investigation both in vitro and in genome editing experiments.
Giang T Nguyen, Michael A Schelling, Akshara Raju, Kathryn A Buscher, Aneisha Sritharan, Dipali G Sashital   +5 more
openaire   +2 more sources

ERA-CRISPR/Cas12a system: a rapid, highly sensitive and specific assay for Mycobacterium tuberculosis

Frontiers in Cellular and Infection Microbiology
IntroductionMycobacterium tuberculosis, the causative agent of human tuberculosis, poses a significant threat to global public health and imposes a considerable burden on the economy. However, existing laboratory diagnostic methods for M.
Tian Gan, Jianwei Yu, Zhongliang Deng, Jun He   +3 more
doaj   +1 more source

Rapid detection of FAdV-4 by one-tube RPA-CRISPR/Cas12a assay

Frontiers in Microbiology
IntroductionFowl adenovirus serotype 4 (FAdV-4) is a highly contagious viral pathogen of global significance that affects various avian species. It primarily infects poultry and wild birds, leading to avian inclusion body hepatitis (IBH) and hepatitis ...
Lei Ma, Xueping Wang, Mingliang Zhang, Mengjie Zhu   +3 more
doaj   +1 more source

CRISPR-Cas12a-based detection and differentiation of Mycobacterium spp

Clinica Chimica Acta
Mycobacterium species cause several vital human diseases, including tuberculosis and non-tuberculous mycobacterial infections, which are treated with different drug regimens Therefore, accurate and rapid diagnosis is essential for effective treatment and controlling the spread of these pathogens.
Peeraphan, Compiro, Nantinee, Chomta, Juthamas, Nimnual, Samitanan, Sunantawanit, Sunchai, Payungporn, Suwatchareeporn, Rotcheewaphan, Pornchai, Keawsapsak   +6 more
openaire   +2 more sources

SWITCHER, a CRISPR-inducible floxed wild-type Cre regulating CRISPR activity

Communications Biology
Although several Cre-regulated CRISPR/Cas platforms exist, a CRISPR/Cas-controlled Cre-system remains a challenge. Here, we present a genetic switch we term SWITCHER based on a floxed wild-type Cre-construct representing a CRISPR-inducible and self ...
Björn Schuster, Ivana Dobiášovská, Jovana Ćurčić, Petr Pajer, Šimon Borna, Petr Bartůněk   +5 more
doaj   +1 more source

Ov-RPA–CRISPR/Cas12a assay for the detection of Opisthorchis viverrini infection in field-collected human feces

Parasites & Vectors
Background Opisthorchis viverrini infection is traditionally diagnosed using the Kato–Katz method and formalin ethyl–acetate concentration technique. However, the limited sensitivity and specificity of these techniques have prompted the exploration of ...
Orawan Phuphisut, Akkarin Poodeepiyasawat, Tippayarat Yoonuan, Dorn Watthanakulpanich, Charin Thawornkuno, Onrapak Reamtong, Megumi Sato, Poom Adisakwattana   +7 more
doaj   +1 more source

Brain‐Targeted Cas12a Ribonucleoprotein Nanocapsules Enable Synergetic Gene Co‐Editing Leading to Potent Inhibition of Orthotopic Glioblastoma

Advanced Science
Gene‐editing technology shows great potential in glioblastoma (GBM) therapy. Due to the complexity of GBM pathogenesis, a single gene‐editing‐based therapy is unlikely to be successful; therefore, a multi‐gene knockout strategy is preferred for effective
Weimin Ruan, Sen Xu, Yang An, Yingxue Cui, Yang Liu, Yibin Wang, Muhammad Ismail, Yong Liu, Meng Zheng   +8 more
doaj   +1 more source

Development of an ERA-CRISPR/Cas12a-based visual diagnostic assay for bovine babesiosis. [PDF]

Front Vet Sci
Li Y, Feng X, Fu X, Kalim F, Li S, Zafar I, Rizk MA, Cui Z, Chahan B, Li Y, Ning D, Guo Q.   +11 more
europepmc   +1 more source

Rapid Visual Detection of Cyprinid Herpesvirus 2 Based on One-Tube RPA-CRISPR/Cas12a System. [PDF]

Vet Sci
Li J, Zou W, Tang Y, Liu M, Liu Y, Dong Y.   +5 more
europepmc   +1 more source