Results 51 to 60 of about 7,068 (190)

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Animal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

CRISPR/Cas12a-Based Biosensing: Advances in Mechanisms and Applications for Nucleic Acid Detection

Biosensors
Nucleic acid detection technology is crucial for molecular diagnosis. The advent of CRISPR/Cas12a-based nucleic acid detection has considerably broadened its scope, from the identification of infectious disease-causing microorganisms to the detection of ...
Kun Du, Qinlong Zeng, Mingjun Jiang, Zhiqing Hu, Miaojin Zhou, Kun Xia   +5 more
doaj   +1 more source

Revolutionizing Healthcare With Paper‐Based Nucleic Acid Testing

Exploration, EarlyView.
This work summarizes strategies to enhance paper‐based devices for nucleic acid testing. Key approaches include optimizing paper platforms, improving nucleic acid amplification, and refining labeling/signaling methods. These advancements aim to boost sensitivity, speed, and usability, making paper‐based diagnostics more effective for point‐of‐care ...
Hong Zhang, Yi Yang, Heng Li, Xiaolin Hu, Hui Xiao, Dou Wang, Sergio Benardini, Wei Gu, Yang Luo   +8 more
wiley   +1 more source

CRISPR-Cas12a exhibits metal-dependent specificity switching

, 2023
Abstract Cas12a is the immune effector of type V-A CRISPR-Cas systems and has been co-opted for genome editing and other biotechnology tools. The specificity of Cas12a has been the subject of extensive investigation both in vitro and in genome editing experiments. However, in vitro studies have often been performed at
Giang T. Nguyen, Michael A. Schelling, Kathryn A. Buscher, Aneisha Sritharan, Dipali G. Sashital   +4 more
openaire   +2 more sources

Borophene‐based sensing platforms: Pioneering ultrasensitive detection

FlexMat, EarlyView.
Schematic Diagram of Performance Optimization Strategies and Sensing Application Characteristics of Two‐Dimensional Boron‐Based Materials in Novel Sensors. Abstract Borophene is an emerging two‐dimensional (2D) material. Since significant breakthroughs were achieved in the experimental synthesis of borophene, the exploration and development of ...
Chi Yuan, Yanpeng Ji, Yi Liu, Guoan Tai
wiley   +1 more source

RPA-CRISPR/Cas12a-Based Detection of Haemophilus parasuis

Animals, 2023
Haemophilus parasuis (H. parasuis, HPS) is a prominent pathogenic bacterium in pig production. Its infection leads to widespread fibrinous inflammation in various pig tissues and organs, often in conjunction with various respiratory virus infections, and leads to substantial economic losses in the pig industry.
Kunli Zhang, Zeyi Sun, Keda Shi, Dongxia Yang, Zhibiao Bian, Yan Li, Hongchao Gou, Zhiyong Jiang, Nanling Yang, Pinpin Chu, Shaolun Zhai, Zhanyong Wei, Chunling Li   +12 more
openaire   +3 more sources

CRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA

Scientific Reports, 2022
CRISPR-Cas12a systems are becoming an attractive genome editing tool for cell engineering due to their broader editing capabilities compared to CRISPR-Cas9 counterparts.
D. J. Jedrzejczyk, L. D. Poulsen, M. Mohr, N. D. Damas, S. Schoffelen, A. Barghetti, R. Baumgartner, B. T. Weinert, T. Warnecke, R. T. Gill   +9 more
doaj   +1 more source

Engineered molecular diagnostic strategies for hepatocellular carcinoma: Integrating flexible photonic barcodes, liquid biopsies, and AI‐enhanced sensing platforms

FlexMat, EarlyView.
Abstract Early diagnosis of hepatocellular carcinoma (HCC) remains challenging because conventional serum biomarkers, especially alpha‐fetoprotein (AFP), show limited sensitivity for early‐stage disease. Recent progress in engineered molecular diagnostics provides new opportunities to improve HCC detection through integrated material, molecular, and ...
Jingzhi Xue, Wanqi Yang, Xiaoyu Liu, Xuehui Chu, Wei Li, Jinglin Wang   +5 more
wiley   +1 more source

Development and clinical application of a novel CRISPR-Cas12a based assay for the detection of African swine fever virus

BMC Microbiology, 2020
Background As no treatment or effective vaccine for African swine fever virus (ASFV) is currently available, a rapid, highly sensitive diagnostic is urgently needed to curb the spread of ASFV.
Xiaoying Wang, Sheng He, Na Zhao, Xiaohong Liu, Yongchang Cao, Guihong Zhang, Gang Wang, Chunhe Guo   +7 more
doaj   +1 more source

Illuminating point‐of‐care diagnostics with aggregation‐induced emission biosensors

FlexMat, EarlyView.
This review highlights the application of aggregation‐induced emission luminogens (AIEgens) in point‐of‐care (POC) diagnostics for disease biomarker detection. It systematically examines a range of biosensing platforms, including lateral flow immunoassays, microfluidic chips, and electrochemiluminescence sensors, alongside their utility in detecting ...
Danni Hu, Yubing Hu, Dong Wang, Ben Zhong Tang   +3 more
wiley   +1 more source