Results 31 to 40 of about 7,068 (190)

Dynamic Operations in Macromolecular Data Storage

Angewandte Chemie, EarlyView.
With the exponential growth of digital data and the limitations of conventional silicon‐based storage and computing technologies, macromolecular data storage, the practice of encoding digital information within large, complex molecules like DNA or synthetic polymers, has emerged as a scalable and sustainable alternative to traditional digital ...
Jakub Ossowski, Oskar Affenberger, Jean‐François Lutz, Maria Nerantzaki   +3 more
wiley   +2 more sources

Accessibility and Alignment of Trans‐Cleavage Substrates Modulate the Kinetics of Cas12a Ribonucleoprotein

Angewandte Chemie, EarlyView.
Two key binding events that collectively determine the catalytic efficiency of trans‐cleavage: (1) the substrate passing through the gap between the REC and NUC domains, determined by Kpass, and (2) the alignment of the substrate with the catalytic triad of the ribonucleoprotein, determined by Kalign.
Jianyu Hu, Wei Feng, Hongquan Zhang, X. Chris Le   +3 more
wiley   +2 more sources

Efficient Targeted Mutagenesis Mediated by CRISPR-Cas12a Ribonucleoprotein Complexes in Maize

Frontiers in Genome Editing, 2021
Recent advances in the development of CRISPR-Cas genome editing technologies have made it possible to perform targeted mutagenesis and precise gene replacement in crop plants. CRISPR-Cas9 and CRISPR-Cas12a are two main types of widely used genome editing
Shujie Dong, Yinping Lucy Qin, Christopher A. Vakulskas, Michael A. Collingwood, Mariam Marand, Stephen Rigoulot, Ling Zhu, Yaping Jiang, Weining Gu, Chunyang Fan, Anna Mangum, Zhongying Chen, Michele Yarnall, Heng Zhong, Sivamani Elumalai, Liang Shi, Qiudeng Que   +16 more
doaj   +1 more source

Development of a CRISPR-Cas12a rapid diagnostic for human cytomegalovirus

Antiviral Research, 2023
Despite decades of research, human cytomegalovirus (CMV) continues to contribute to significant morbidity and mortality in transplant settings and remains the leading cause of viral congenital infections. Clinical diagnosis of CMV infection and/or reactivation under these settings is completed using real time quantitative polymerase chain reaction (RT ...
Chandler H. Monk, Brady M. Youngquist, Alyson D. Brady, Jeffrey G. Shaffer, Tony Y. Hu, Bo Ning, Kevin J. Zwezdaryk   +6 more
openaire   +2 more sources

CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement

Frontiers in Plant Science, 2020
Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion from today. To meet the demands of growing, geographically and socio-economically diversified nations, we need to diversity and expand agricultural production ...
Anindya Bandyopadhyay, Nagesh Kancharla, Vivek S. Javalkote, Santanu Dasgupta, Thomas P. Brutnell, Thomas P. Brutnell   +5 more
doaj   +1 more source

Ultra-sensitive detection of ecologically rare fish from eDNA samples based on the RPA-CRISPR/Cas12a technology

iScience, 2023
Summary: Environmental DNA (eDNA) research holds great promise for improving biodiversity science and conservation efforts by enabling worldwide species censuses in near real-time.
Xing-Yi Wei, Li Liu, Huan Hu, Huang-Jie Jia, Ling-Kang Bu, De-Sheng Pei   +5 more
doaj   +1 more source

Rapid detection of feline parvovirus using RAA-CRISPR/Cas12a-based lateral flow strip and fluorescence

Frontiers in Microbiology
Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats, with high morbidity and mortality, necessitating a rapid and effective antigen diagnostic test with high sensitivity and specificity. In this study, a diagnostic platform based
Han Chen, Hailing Zhang, Jie Guo, Xiangshu Meng, Mengfan Yao, Longbin He, Xiaoxuan Nie, Han Xu, Chao Liu, Jian Sun, Jian Sun, Fei Wang, Yuelong Sun, Zhong Jiang, Yanliang He, Jianlou Zhang, Jianke Wang   +16 more
doaj   +1 more source

Immunocapture Magnetic Beads Enhanced the LAMP-CRISPR/Cas12a Method for the Sensitive, Specific, and Visual Detection of Campylobacter jejuni

Biosensors, 2022
Campylobacter jejuni is one of the most important causes of food-borne infectious disease, and poses challenges to food safety and public health. Establishing a rapid, accurate, sensitive, and simple detection method for C. jejuni enables early diagnosis,
Chao Li, Xuan Chen, Renqiao Wen, Peng Ma, Kui Gu, Cui Li, Changyu Zhou, Changwei Lei, Yizhi Tang, Hongning Wang   +9 more
doaj   +1 more source

CRISPR/Cas12a‐mediated genome engineering in the photosynthetic bacterium Rhodobacter capsulatus

Microbial Biotechnology, 2021
Summary Purple non‐sulfur photosynthetic bacteria (PNSB) such as Rhodobacter capsulatus serve as a versatile platform for fundamental studies and various biotechnological applications.
Yang Zhang, Jifeng Yuan
doaj   +1 more source

A CRISPR Test for Rapidly and Sensitively Detecting Circulating EGFR Mutations

Diagnostics, 2020
The detection of EGFR mutations in circulating cell-free DNA can enable personalized therapy for cancer. The current techniques for detecting circulating EGFR mutations are expensive and time-consuming with moderate sensitivity.
Jen-Hui Tsou, Qixin Leng, Feng Jiang
doaj   +1 more source