Results 11 to 20 of about 14,674 (226)

CRISPR/Cas12a with Universal crRNA for Indiscriminate Virus Detection

Molecules
Viruses, known for causing widespread biological harm and even extinction, pose significant challenges to public health. Virus detection is crucial for accurate disease diagnosis and preventing the spread of infections.
Zhenlin Shang, Sitong Liu, Dongxu Liu, Xiaojing Pei, Shujing Li, Yifan He, Yigang Tong, Guoqi Liu   +7 more
doaj   +4 more sources

Harnessing noncanonical crRNA for highly efficient genome editing

Nature Communications
The CRISPR-Cas12a system is more advantageous than the widely used CRISPR-Cas9 system in terms of specificity and multiplexibility. However, its on-target editing efficiency is typically much lower than that of the CRISPR-Cas9 system.
Guanhua Xun, Zhixin Zhu, Nilmani Singh, Jingxia Lu, Piyush K. Jain, Huimin Zhao   +5 more
doaj   +4 more sources

crRNA Biogenesis

, 2012
Mature crRNAs are key elements in CRISPR-Cas defense against genome invaders. These short RNAs are composed of unique repeat/spacer sequences that guide the Cas protein(s) to the cognate invading nucleic acids for their destruction. The biogenesis of mature crRNAs involves highly precise processing events.
Charpentier, E., van der Oost, J., White, M.   +2 more
core   +4 more sources

Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo [PDF]

Nature Communications, 2018
AbstractEngineering of the Cpf1 crRNA has the potential to enhance its gene editing efficiency and non-viral delivery to cells. Here, we demonstrate that extending the length of its crRNA at the 5′ end can enhance the gene editing efficiency of Cpf1 both in cells and in vivo. Extending the 5′ end of the crRNA enhances the gene editing efficiency of the
Hyo Min Park, Hui Liu, Joann Wu, Anthony Chong, Vanessa Mackley, Christof Fellmann, Anirudh Rao, Fuguo Jiang, Hunghao Chu, Niren Murthy, Kunwoo Lee   +10 more
openaire   +7 more sources

Kinetic dissection of pre-crRNA binding and processing by CRISPR–Cas12a

RNA
CRISPR–Cas12a binds and processes a single pre-crRNA during maturation, providing a simple tool for genome editing applications. Here, we constructed a kinetic and thermodynamic framework for pre-crRNA processing by Cas12a in vitro, and we measured the contributions of distinct regions of the pre-crRNA to this reaction.
Selma Sinan, Nathan M. Appleby, Chia-Wei Chou, Ilya J. Finkelstein, Rick Russell   +4 more
openaire   +3 more sources

Tailoring Cas12a functionality with a user-friendly and versatile crRNA variant toolbox

Nature Communications
Cas12a, with its unique targeting and cleavage activity towards DNA, has been widely applied in gene editing and molecular diagnostics. However, there currently lacks an activity regulation strategy that combines flexibility and simplicity to adapt ...
Jing Han, Yuan Min, Lan Hu, Jie-jie Chen, Shu-Xia Zhang, Xiao-Fan Li, Zhou-Hua Cheng, Dong-Feng Liu, Han-Qing Yu   +8 more
doaj   +2 more sources

Single-Molecule Imaging of Endogenous RNA Using a Miniaturized and Circularly Permuted CRISPR-Cas13 Degron-Masking System CDegSR. [PDF]

Adv Sci (Weinh)
CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shao S, Zhang H.
europepmc   +2 more sources

FnCas12a/crRNA-Mediated Genome Editing in Eimeria tenella [PDF]

Frontiers in Genetics, 2021
Eimeria species are intracellular parasites residing inside the intestinal epithelial cell, which cause poultry coccidiosis and result in significant financial losses in the poultry industry. Genome editing of Eimeria is of immense importance for the development of vaccines and drugs. CRISPR/Cas9 has been utilized for manipulating the genome of Eimeria
Peipei Cheng, Zhihao Zhang, Fayu Yang, Shuo Cai, Lina Wang, Chunmei Wang, Mi Wang, Yingchun Liu, Chenzhong Fei, Lifang Zhang, Feiqun Xue, Feng Gu   +11 more
openaire   +3 more sources

Improvement of the LbCas12a-crRNA System for Efficient Gene Targeting in Tomato [PDF]

Frontiers in Plant Science, 2021
Plant gene targeting (GT) can be utilized to precisely replace up to several kilobases of a plant genome. Recent studies using the powerful clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) nucleases significantly improved plant GT efficiency.
Tien Van Vu, Tien Van Vu, Duong Thi Hai Doan, Mil Thi Tran, Mil Thi Tran, Yeon Woo Sung, Young Jong Song, Jae-Yean Kim, Jae-Yean Kim   +8 more
openaire   +3 more sources

Recognition of the crRNA by Lb2Cas12a.

, 2023
(A) Representation of the Lb2Cas12a-bound crRNA. Cartoon shown in the electron density map (2mFo-DFc, gray for crRNA and blue for Mg2+ and H2O), contoured at 1.0 σ, base-pairs shown as stick representation. (B) Representation of the Lb2Cas12a-bound crRNA
Sun Meng (11007860), Shi Jian (14790680), Yuren Adam Yuan (14790683), Chacko Jobichen (43238), Satoru Machida (14790671), J. Sivaraman (31440), Li Jianwei (14790668), Randy J. Read (14790674), Chen Hongying (14790677)   +8 more
core   +2 more sources