Results 41 to 50 of about 14,674 (226)

Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics

Proceedings of the National Academy of Sciences, 2022
CRISPR diagnostics based on nucleic acid amplification faces barriers to its commercial use, such as contamination risks and insufficient sensitivity. Here, we propose a robust solution involving optochemical control of CRISPR RNA (crRNA) activation in CRISPR detection.
Menglu Hu, Zhiqiang Qiu, Zirong Bi, Tian Tian, Yongzhong Jiang, Xiaoming Zhou   +5 more
openaire   +2 more sources

Structural constraints and enzymatic promiscuity in the Cas6-dependent generation of crRNAs [PDF]

Nucleic Acids Research, 2016
A hallmark of defense mechanisms based on clustered regularly interspaced short palindromic repeats (CRISPR) and associated sequences (Cas) are the crRNAs that guide these complexes in the destruction of invading DNA or RNA. Three separate CRISPR-Cas systems exist in the cyanobacterium Synechocystis sp. PCC 6803.
Reimann, Viktoria, Alkhnbashi, Omer S., Saunders, Sita Johanna, Scholz, Ingeborg, Hein, Stephanie, Backofen, Rolf, Hess, Wolfgang R.   +6 more
openaire   +3 more sources

Programmable type III-A CRISPR-Cas DNA targeting modules. [PDF]

PLoS ONE, 2017
The CRISPR-Cas systems provide invader defense in a wide variety of prokaryotes, as well as technologies for many powerful applications. The Type III-A or Csm CRISPR-Cas system is one of the most widely distributed across prokaryotic phyla, and cleaves ...
H Travis Ichikawa, John C Cooper, Leja Lo, Jason Potter, Rebecca M Terns, Michael P Terns   +5 more
doaj   +1 more source

PrimedSherlock: A tool for rapid design of highly specific CRISPR-Cas12 crRNAs

BMC Bioinformatics, 2022
Abstract Background CRISPR-Cas based diagnostic assays provide a portable solution which bridges the benefits of qRT-PCR and serological assays in terms of portability, specificity and ease of use. CRISPR-Cas assays are rapidly fieldable, specific and have been rigorously validated against a number ...
James G. Mann, R. Jason Pitts
openaire   +4 more sources

Design, Modeling and Computational Analysis of crRNA to Regulate MetastamiR-10b and MetastamiR-126 in Post-transcriptional Level by CRISPR-C2c2 (Cas13a) Technique

مجله انفورماتیک سلامت و زیست پزشکی, 2020
Introduction: Metastasis is one the most important causes of mortality in cancer patients. Recent studies have shown the metastatic potential of a specific group of microRNAs called metastamirs.
Fatemeh Ebrahimi Tarki, Mahsa Bourbour, Mahboobe Zarrabi   +2 more
doaj  

Potent programmable antiviral against dengue virus in primary human cells by Cas13b RNP with short spacer and delivery by VLP

Molecular Therapy: Methods & Clinical Development, 2021
With sequencing as a standard frontline protocol to identify emerging viruses such Zika virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), direct utilization of sequence data to program antivirals against the viruses could accelerate
Ekapot Singsuksawat, Suppachoke Onnome, Pratsaneeyaporn Posiri, Amporn Suphatrakul, Nittaya Srisuk, Rapirat Nantachokchawapan, Hansa Praneechit, Chutimon Sae-kow, Pala Chidpratum, Khanit Sa-ngiamsuntorn, Suradej Hongeng, Panisadee Avirutnan, Thaneeya Duangchinda, Bunpote Siridechadilok   +13 more
doaj   +1 more source

Lactobacillus gasseri CRISPR-Cas9 characterization In Vitro reveals a flexible mode of protospacer-adjacent motif recognition. [PDF]

PLoS ONE, 2018
While the CRISPR-Cas9 system from S. pyogenes is a powerful genome engineering tool, additional programmed nucleases would enable added flexibility in targeting space and multiplexing. Here, we characterized a CRISPR-Cas9 system from L. gasseri and found
Emily M Anderson, Shawn McClelland, Elena Maksimova, Žaklina Strezoska, Megan Basila, Alexandra E Briner, Rodolphe Barrangou, Anja van Brabant Smith   +7 more
doaj   +1 more source

Stabilizing Functional Nucleic Acids for Real‐Sample Diagnostics

Angewandte Chemie, EarlyView.
Functional nucleic acids enable programmable real‐sample diagnostics, but often lose function in complex biological matrices. This review highlights four stabilization levers—chemical, topological, architectural, and microenvironment/interface engineering—that preserve aptamer and DNAzyme performance in saliva, serum, urine, and stool, reframing ...
Soyeon Lee, Letizia Dondi, Amal Mathai, Connor Nurmi, Yingfu Li   +4 more
wiley   +2 more sources

Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a

, 2023
ABSTRACT CRISPR-Cas12a binds and processes a single pre-crRNA during maturation, providing a simple tool for genome editing applications. Here, we constructed a kinetic and thermodynamic framework for pre-crRNA processing by Cas12a in vitro , and we measured the contributions of ...
Selma Sinan, Nathan M. Appleby, Rick Russell   +2 more
openaire   +2 more sources

CRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA

Scientific Reports, 2021
AbstractCRISPR-Cas12a systems are becoming an attractive genome editing tool for cell engineering due to their broader editing capabilities compared to CRISPR-Cas9 counterparts. As opposed to Cas9, the Cas12a endonucleases are characterized by a lack of trans-activating crRNA (tracrRNA), which reduces the complexity of the editing system and ...
Jedrzejczyk, D. J., Poulsen, L. D., Mohr, M., Damas, N. D., Schoffelen, S.; id_orcid 0000-0003-2664-8561, Barghetti, A., Baumgartner, R., Weinert, B. T., Warnecke, T., Gill, R. T.   +9 more
openaire   +3 more sources