Results 51 to 60 of about 14,674 (226)
Cartoon model showing dynamic apo Lb2Cas12a and crRNA binding.
, 2023 The conformational changes induced by crRNA binding are shown in this order: (1) Overall dynamic of apo Lb2Cas12a; (2) the process of crRNA binding; (3) stabilization of Lb2Cas12a-crRNA in compact conformation; (4) Met493~Leu523 undergoing major ...
Sun Meng (11007860) +8 more
core +1 more source
Angewandte Chemie, EarlyView.Two key binding events that collectively determine the catalytic efficiency of trans‐cleavage: (1) the substrate passing through the gap between the REC and NUC domains, determined by Kpass, and (2) the alignment of the substrate with the catalytic triad of the ribonucleoprotein, determined by Kalign.
Jianyu Hu +3 more
wiley +2 more sources
Synthetic Oligonucleotides Inhibit CRISPR-Cpf1-Mediated Genome Editing
Cell Reports, 2018 Summary: Previously, researchers discovered a series of anti-CRISPR proteins that inhibit CRISPR-Cas activity, such as Cas9 and Cpf1 (Cas12a). Herein, we constructed crRNA variants consisting of chemically modified DNA-crRNA and RNA-crRNA duplexes and ...
Bin Li +7 more
doaj +1 more source
Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish. [PDF]
PLoS ONE, 2015 The type II clustered regularly interspaced short palindromic repeats (CRISPR) associated with Cas9 endonuclease (CRISPR/Cas9) has become a powerful genetic tool for understanding the function of a gene of interest.
Hirohito Kotani +4 more
doaj +1 more source
The Versatile Tool: CRISPR/Cas12a System for Nucleic Acid Detection
Shengwu huaxue yu shengwu wuli jinzhanThe CRISPR/Cas system consists of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (Cas). The system forms an adaptive immune system in archaea and bacteria.
DANG Sheng, ZHANG Shuai, ZHAI Jing-Bo
doaj +1 more source
Cell Reports, 2019 Summary: The type III-A Csm complex of Streptococcus thermophilus (StCsm) provides immunity against invading nucleic acids through the coordinated action of three catalytic domains: RNase (Csm3), ssDNase (Cas10-HD), and cyclic oligoadenylates synthase ...
Irmantas Mogila +5 more
doaj +1 more source
Highly specific chimeric DNA-RNA-guided genome editing with enhanced CRISPR-Cas12a system
Molecular Therapy: Nucleic Acids, 2022 The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a system is composed of a Cas12a effector that acts as a DNA-cleaving endonuclease and a crispr RNA (crRNA) that guides the effector to the target DNA.
Hanseop Kim +18 more
doaj +1 more source
Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation
Molecular Oncology, EarlyView.How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim +11 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A CRISPR RNA Is Closely Related With the Size of the Cascade Nucleoprotein Complex
Frontiers in Microbiology, 2019 The currently known prokaryotic adaptive immune system against mobile genetic elements is based on clustered regularly interspaced short palindromic repeats (CRISPR).
Do-Heon Gu, Sung Chul Ha, Jeong-Sun Kim
doaj +1 more source