Results 71 to 80 of about 14,674 (226)

A small library crRNA-enhanced CRISPR-Cas12a system for ultrasensitive point-of-care test of hantavirus M gene

Sensing and Bio-Sensing Research
The integration of CRISPR-Cas systems with isothermal nucleic acid amplification (INA) holds transformative potential for point-of-care diagnostics, yet technical challenges such as limited sensitivity, cross-contamination risks, and incompatibility ...
Jian Zhou, Xue-mei Ren, Xin Wang, Pu Xu, Zhuo Li   +4 more
doaj   +1 more source

CRISPR transcript processing: a mechanism for generating a large number of small interfering RNAs

Biology Direct, 2012
Background CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated sequences) is a recently discovered prokaryotic defense system against foreign DNA, including viruses and plasmids.
Djordjevic Marko, Djordjevic Magdalena, Severinov Konstantin   +2 more
doaj   +1 more source

Systematic Position Mapping of Split CRISPR‐Cas12a Activators Enables Highly Sensitive miRNA Detection and Cancer Cell Stratification

Advanced Science, EarlyView.
ABSTRACT Amplification‐free Cas12a diagnostics with split crRNA enable rapid and programmable target recognition, yet insufficient understanding of DNA activator architecture prevents predictable control over trans‐cleavage activity and sensitivity. Here we systematically map over 200 split DNA activator configurations by introducing nicks at every ...
Xiaoyan Tang, Zhe Li, Yuning Lu, Miao Ma, Zijian Mo, Jiajun Ke, Xinyu Luan, Tiangang Luan, Junqiu Zhai   +8 more
wiley   +1 more source

Profiling crRNA architectures for enhanced Cas12 biosensing

Communications Biology
Abstract CRISPR-Cas diagnostics are revolutionizing point-of-care molecular testing due to the programmability, simplicity, and sensitivity of Cas systems with trans-cleavage activity. CRISPR-Cas12 assays are promising for detecting single nucleotide polymorphisms (SNPs).
Elizabeth Toyin Ajibode, Alexandra R. Bender, Kevin Yehl   +2 more
openaire   +3 more sources

Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array [PDF]

Nature Biotechnology, 2016
Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain,
Bernd Zetsche, Matthias Heidenreich, Prarthana Mohanraju, Iana Fedorova, Jeroen Kneppers, Ellen M DeGennaro, Nerges Winblad, Sourav R Choudhury, Omar O Abudayyeh, Jonathan S Gootenberg, Wen Y Wu, David A Scott, Konstantin Severinov, John van der Oost, Feng Zhang   +14 more
openaire   +5 more sources

Optimized Cas9‐Enriched Nanopore Sequencing and Analysis Workflow for Clinical Diagnosis of Repeat Expansion Disorders

Advanced Science, EarlyView.
An optimized Cas9‐enriched nanopore sequencing workflow, combined with STRiker, enables simultaneous analysis of disease‐associated STR loci from patient blood. The nCATS–STRiker workflow detects repeat expansions, de novo repeat motifs, interruption patterns, and methylation in a single assay, improving the genetic diagnosis of previously undiagnosed ...
Seungbok Lee, Chanju Jung, Minjeong Kim, Narae Kim, Gue‐Ho Hwang, Soon‐Tae Lee, Kon Chu, Sang Kun Lee, Han‐Joon Kim, Jong‐Hee Chae, Sangsu Bae, Jangsup Moon   +11 more
wiley   +1 more source

Analysis of crRNA Using Liquid Chromatography Electrospray Ionization Mass Spectrometry (LC ESI MS)

, 2015
Mass spectrometry is a powerful tool for characterizing RNA. Here we describe a method for the identifi cation and characterisation of crRNA using liquid chromatography interfaced with electrospray ionization mass spectrometry (LC ESI MS).
Dickman, Mark J., Nwokeoji, Alison O., Sakharam P. Waghmare, Waghmare, Sakharam P., Alison O. Nwokeoji, Mark J. Dickman   +5 more
core   +1 more source

Efficient generation of locus-specific human CAR-T cells with CRISPR/cCas12a

STAR Protocols, 2022
Summary: We recently developed a system to create human chimeric antigen receptor (CAR)-T cells using conjugated Cas12a (cCas12a) in which Cas12a is covalently linked to its CRISPR RNA (crRNA). This protocol describes site-specific modification of Cas12a
Xinyu Ling, Liying Chang, Heqi Chen, Tao Liu   +3 more
doaj   +1 more source

A comparison of the interactions of bacterial and archaeal Csm5 with target and crRNA.

, 2023
(A) A sharp kink in the peptide backbone of the loop region following β-strand 1 facilitates an interaction with crRNA. A sequence logo depicting the conservation of β1-loop is show above a superposition of S.
Asma Hatoum-Aslan (4451431), Jack A. Dunkle (2091862), Scott M. Stagg (1281813), Mohammadreza Paraan (16436961), Lucy Chou-Zheng (16436964), Sarah A. Khweis (14173444), Allyn J. Schoeffler (16436967), Mohamed Nasef (14173441)   +7 more
core   +1 more source

Asymmetric CRISPR enabling cascade signal amplification for nucleic acid detection by competitive crRNA

Nature Communications, 2023
Nucleic acid detection powered by CRISPR technology provides a rapid, sensitive, and deployable approach to molecular diagnostics. While exciting, there remain challenges limiting its practical applications, such as the need for pre-amplification and the
Jeong Moon, Changchun Liu
doaj   +1 more source